1887

Abstract

Strain W126, a Gram-reaction-positive, spore-forming, rod-shaped, facultatively anaerobic bacterium, motile by means of peritrichous flagella, was isolated from selenium mineral soil in Hubei province of China. 16S rRNA gene sequence analysis demonstrated that this isolate belonged to the genus , with 97.9 % sequence similarity to MH21, while compared with the other species of the genus , the 16S rRNA gene sequence similarities were less than 96.0 %. DNA–DNA hybridization between strain W126 and DSM 15890 was 24 %. The major isoprenoid menaquinone was menaquinone-7. Anteiso-C was the major fatty acid. The DNA G+C content was 42.3 mol%. The major polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown aminophospholipids and an unknown lipid. Strain W126 contained A1γ--diaminopimelic acid in the cell-wall peptidoglycan. The phenotypic, chemotaxonomic and genotypic data indicate that strain W126 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is W126 ( = KCTC 33420 = CCTCC AB 2014003).

Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.063701-0
2014-08-01
2019-10-16
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2662.html?itemId=/content/journal/ijsem/10.1099/ijs.0.063701-0&mimeType=html&fmt=ahah

References

  1. Ahmed I., Yokota A., Yamazoe A., Fujiwara T.. ( 2007;). Proposal of Lysinibacillus boronitolerans gen. nov. sp. nov., and transfer of Bacillus fusiformis to Lysinibacillus fusiformis comb. nov. and Bacillus sphaericus to Lysinibacillus sphaericus comb. nov.. Int J Syst Evol Microbiol 57:, 1117–1125. [CrossRef][PubMed]
    [Google Scholar]
  2. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D.. ( 1991;). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit-ribosomal RNA sequences. . Lett Appl Microbiol 13:, 202–206. [CrossRef]
    [Google Scholar]
  3. Ash C., Priest F. G., Collins M. D.. ( 1993;). Molecular identification of rRNA group 3 bacilli (Ash, Farrow, Wallbanks and Collins) using a PCR probe test. Proposal for the creation of a new genus Paenibacillus. . Antonie van Leeuwenhoek 64:, 253–260. [CrossRef][PubMed]
    [Google Scholar]
  4. Ash C., Priest F. G., Collins M. D.. ( 1994;). Paenibacillus gen. nov. and Paenibacillus polymyxa comb. nov. In Validation of the Publication of New Names and New Combinations Previously Effectively Published Outside the IJSB, List no. 51. . Int J Syst Bacteriol 44:, 852. [CrossRef]
    [Google Scholar]
  5. Benardini J. N., Vaishampayan P. A., Schwendner P., Swanner E., Fukui Y., Osman S., Satomi M., Venkateswaran K.. ( 2011;). Paenibacillus phoenicis sp. nov., isolated from the Phoenix Lander assembly facility and a subsurface molybdenum mine. . Int J Syst Evol Microbiol 61:, 1338–1343. [CrossRef][PubMed]
    [Google Scholar]
  6. Chung Y. R., Kim C. H., Hwang I., Chun J.. ( 2000;). Paenibacillus koreensis sp. nov., a new species that produces an iturin-like antifungal compound. . Int J Syst Evol Microbiol 50:, 1495–1500. [CrossRef][PubMed]
    [Google Scholar]
  7. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  8. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  9. Cowan S. T., Steel K. J.. ( 1965;). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  10. Daane L. L., Harjono I., Barns S. M., Launen L. A., Palleron N. J., Häggblom M. M.. ( 2002;). PAH-degradation by Paenibacillus spp. and description of Paenibacillus naphthalenovorans sp. nov., a naphthalene-degrading bacterium from the rhizosphere of salt marsh plants. . Int J Syst Evol Microbiol 52:, 131–139.[PubMed]
    [Google Scholar]
  11. Dong X. Z., Cai M. Y.. ( 2001;). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press;.
    [Google Scholar]
  12. Dussault H. P.. ( 1955;). An improved technique for staining red halophilic bacteria. . J Bacteriol 70:, 484–485.[PubMed]
    [Google Scholar]
  13. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  14. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  15. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  16. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  17. Horn M. A., Ihssen J., Matthies C., Schramm A., Acker G., Drake H. L.. ( 2005;). Dechloromonas denitrificans sp. nov., Flavobacterium denitrificans sp. nov., Paenibacillus anaericanus sp. nov. and Paenibacillus terrae strain MH72, N2O-producing bacteria isolated from the gut of the earthworm Aporrectodea caliginosa. . Int J Syst Evol Microbiol 55:, 1255–1265. [CrossRef][PubMed]
    [Google Scholar]
  18. Kim B. C., Lee K. H., Kim M. N., Kim E. M., Min S. R., Kim H. S., Shin K. S.. ( 2009;). Paenibacillus pini sp. nov., a cellulolytic bacterium isolated from the rhizosphere of pine tree. . J Microbiol 47:, 699–704. [CrossRef][PubMed]
    [Google Scholar]
  19. Kim K. K., Lee K. C., Lee J. S.. ( 2011;). Reclassification of Paenibacillus ginsengisoli as a later heterotypic synonym of Paenibacillus anaericanus. . Int J Syst Evol Microbiol 61:, 2101–2106. [CrossRef][PubMed]
    [Google Scholar]
  20. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  21. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  22. Kroppenstedt R. M.. ( 1982;). Separation of bacterial menaquinones by HPLC using reverse phase (RP18) and a silver loaded ion exchanger as stationary phases. . J Liq Chromatogr 5:, 2359–2367. [CrossRef]
    [Google Scholar]
  23. Kroppenstedt R. M.. ( 1985;). Fatty acid and menaquinone analysis of actinomycetes and related organisms. . In Chemical Methods in Bacterial Systematics (Society for Applied Bacteriology Technical Series vol. 20), pp. 173–199. Edited by Goodfellow M., Minnikin D. E... New York:: Academic Press;.
    [Google Scholar]
  24. Lee J. C., Yoon K. H.. ( 2008;). Paenibacillus woosongensis sp. nov., a xylanolytic bacterium isolated from forest soil. . Int J Syst Evol Microbiol 58:, 612–616. [CrossRef][PubMed]
    [Google Scholar]
  25. Lim J. M., Jeon C. O., Lee J. C., Xu L. H., Jiang C. L., Kim C. J.. ( 2006;). Paenibacillus gansuensis sp. nov., isolated from desert soil of Gansu Province in China. . Int J Syst Evol Microbiol 56:, 2131–2134. [CrossRef][PubMed]
    [Google Scholar]
  26. Liu H. L., Song Y. M., Chen F., Zheng S. X., Wang G.. ( 2013;). Lysinibacillus manganicus sp. nov., isolated from manganese mining soil. . Int J Syst Evol Microbiol 63:, 3568–3573. [CrossRef][PubMed]
    [Google Scholar]
  27. Logan N. A., Berge O., Bishop A. H., Busse H.-J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L.. & other authors ( 2009;). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59:, 2114–2121. [CrossRef][PubMed]
    [Google Scholar]
  28. Ludwig W., Schleifer K. H., Whitman W. B.. ( 2009;). Family IV. Paenibacillaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 3, p. 269. Edited by De Vos P., Garrity G. M., Jones D., Krieg N. R., Ludwig W., Rainey F., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  29. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  30. Okkenhaug G., Zhu Y. G., Luo L., Lei M., Li X., Mulder J.. ( 2011;). Distribution, speciation and availability of antimony (Sb) in soils and terrestrial plants from an active Sb mining area. . Environ Pollut 159:, 2427–2434. [CrossRef][PubMed]
    [Google Scholar]
  31. Priest F. G.. ( 2009;). Genus I. Paenibacillus. . In Bergey’s Manual of Systematic Bacteriology, pp. 269–295. Edited by De Vos G. P., Jones D., Krieg N. R., Ludwig W., Rainey F. A., Schleifer K. H., Whitman W. B... New York:: Springer;.
    [Google Scholar]
  32. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  33. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
    [Google Scholar]
  34. Schumann P.. ( 2011;). Peptidoglycan structure. . Methods Microbiol 38:, 101–129. [CrossRef]
    [Google Scholar]
  35. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K.. ( 1997;). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. . Int J Syst Bacteriol 47:, 289–298. [CrossRef][PubMed]
    [Google Scholar]
  36. Tamaoka J., Komagata K.. ( 1984;). Determination of DNA base composition by reversed-phase high-performance liquid chromatography. . FEMS Microbiol Lett 25:, 125–128. [CrossRef]
    [Google Scholar]
  37. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  38. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  39. Tindall B. J., Rosselló-Móra R., Busse H. J., Ludwig W., Kämpfer P.. ( 2010;). Notes on the characterization of prokaryote strains for taxonomic purposes. . Int J Syst Evol Microbiol 60:, 249–266. [CrossRef][PubMed]
    [Google Scholar]
  40. Valverde A., Peix A., Rivas R., Velázquez E., Salazar S., Santa-Regina I., Rodríguez-Barrueco C., Igual J. M.. ( 2008;). Paenibacillus castaneae sp. nov., isolated from the phyllosphere of Castanea sativa Miller. . Int J Syst Evol Microbiol 58:, 2560–2564. [CrossRef][PubMed]
    [Google Scholar]
  41. Yao R., Wang R., Wang D., Su J., Zheng S. X., Wang G.. ( 2014;). Paenibacillus selenitireducens sp. nov., a selenite-reducing bacterium isolated from a selenium mineral soil. . Int J Syst Evol Microbiol 64:, 805–811. [CrossRef][PubMed]
    [Google Scholar]
  42. Zhou Y., Gao S., Wei D. Q., Yang L. L., Huang X., He J., Zhang Y. J., Tang S. K., Li W. J.. ( 2012;). Paenibacillus thermophilus sp. nov., a novel bacterium isolated from a sediment of hot spring in Fujian province, China. . Antonie van Leeuwenhoek 102:, 601–609. [CrossRef][PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.063701-0
Loading
/content/journal/ijsem/10.1099/ijs.0.063701-0
Loading

Data & Media loading...

Supplements

Supplementary Material 

PDF

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error