1887

Abstract

A mesophilic, slightly halophilic, obligately methylotrophic, methanogenic archaeon, designated strain GTA13, was isolated from natural gas-bearing confined aquifers in the Minami-Kanto gas field, Japan. The cells were non-motile, slightly irregular cocci, 0.7–1.0 µm in diameter and occurred singly, in pairs or as small aggregates. The cells grew with tri- or dimethylamine but not with H/CO, formate, acetate, methanol or dimethyl sulphide. Vitamins, sodium and magnesium were required for growth. Optimal growth occurred at pH 7.0–7.5, 35 °C, 0.35–0.40 M NaCl and 15–50 mM MgCl. The NaCl range for growth was 0.2–1.3 M. The DNA G+C content was 43.7 mol%. Strain GTA13 showed highest levels of 16S rRNA gene sequence similarity with FDF-1 (96.4 % sequence similarity) and DSM 3094 (96.0 %). On the basis of physiological and phylogenetic features, strain GTA13 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GTA13 ( = NBRC 110099 = DSM 28452). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , Japan Oil, Gas and Metals National Corporation (JOGMEC)
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2014-06-01
2020-07-09
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