1887

Abstract

A Gram-stain-positive, strictly aerobic, yellow colony-forming bacterium, designated strain 3-3, was isolated from forest soil of Bac Kan Province in Vietnam. Cells were non-motile rods without gliding motility, showing oxidase- and catalase-positive reactions. Growth was observed at 20–37 °C (optimum, 28 °C) and pH 5.5–9.5 (optimum, pH 7.5). The major cellular fatty acids were iso-C, iso-C G and summed feature 3 (comprising Cω6 and/or Cω7). Strain 3-3 contained phosphatidylethanoamine, three unidentified aminolipids and three unidentified lipids. The G+C content of the genomic DNA was 49.5 mol% and the only isoprenoid quinone detected was menaquinone 7 (MK-7). Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 3-3 formed a tight phylogenetic lineage with T41 with a bootstrap value of 100 %. Strain 3-3 was related most closely to T41 with 97.3 % 16S rRNA gene sequence similarity and the level of DNA–DNA relatedness between the two was 9.4±1.2 %. Based on phenotypic, chemotaxonomic and molecular features, strain 3-3 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is 3-3 ( = KACC 17917 = JCM 19891).

Funding
This study was supported by the:
  • , National Institute of Biological Resources (NIBR)
  • , Ministry of Environment (MOE) , (Award 2013-02-004)
  • , Chung-Ang University
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2014-08-01
2020-07-09
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