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Abstract

Strain KIS2-16 was isolated from a soil sample collected from Daecheong Island of Incheon region, South Korea. KIS2-16 was Gram-staining-positive, aerobic, non-spore-forming, non-motile, catalase-positive, oxidase-negative and mesophilic. On the basis of 16S rRNA gene sequence analysis, strain KIS2-16 represented a member of the genus , being most closely related to the type strains of species of the genus , RP-B30 (97.8 % sequence similarity) and RP-B26 (97.0 %). The fatty acid profile of KIS2-16 was dominated by Cω9, Cω8, C, C 10-methyl (TBSA), C 2-OH and C 2-OH. The major isoprenoid quinone was MK-8(H), and the major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol. The peptidoglycan structure was A3γ-type with -diaminopimelic acid. The genomic DNA G+C content of KIS2-16 was 64.9 mol%. Strain KIS2-16 showed DNA–DNA hybridization values of less than 70 % with the closely related species of the genus . Based on phenotypic, genotypic and phylogenetic data, the isolate represents a novel species of the genus , for which the name sp. nov. (type strain KIS2-16 = DSM 27136 = KACC 17297 = NBRC 109597) is proposed.

Funding
This study was supported by the:
  • , National Academy of Agricultural Science, Rural Development Administration, Republic of Korea , (Award project no. PJ008666)
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2014-12-01
2020-09-26
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