%0 Journal Article %A Ramírez-Bahena, Martha-Helena %A Cuesta, Maria José %A Flores-Félix, José David %A Mulas, Rebeca %A Rivas, Raúl %A Castro-Pinto, Joao %A Brañas, Javier %A Mulas, Daniel %A González-Andrés, Fernando %A Velázquez, Encarna %A Peix, Álvaro %T Pseudomonas helmanticensis sp. nov., isolated from forest soil %D 2014 %J International Journal of Systematic and Evolutionary Microbiology, %V 64 %N Pt_7 %P 2338-2345 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.063560-0 %I Microbiology Society, %X A bacterial strain, OHA11T, was isolated during the course of a study of phosphate-solubilizing bacteria occurring in a forest soil from Salamanca, Spain. The 16S rRNA gene sequence of strain OHA11T shared 99.1 % similarity with respect to Pseudomonas baetica a390T, and 98.9 % similarity with the type strains of Pseudomonas jessenii , Pseudomonas moorei , Pseudomonas umsongensis , Pseudomonas mohnii and Pseudomonas koreensis . The analysis of housekeeping genes rpoB, rpoD and gyrB confirmed its phylogenetic affiliation to the genus Pseudomonas and showed similarities lower than 95 % in almost all cases with respect to the above species. Cells possessed two polar flagella. The respiratory quinone was Q9. The major fatty acids were C16 : 0, C18 : 1ω7c and summed feature 3 (C16 : 1ω7c/iso-C15 : 0 2-OH). The strain was oxidase-, catalase- and urease-positive, positive for arginine dihydrolase but negative for nitrate reduction, β-galactosidase production and aesculin hydrolysis. It was able to grow at 31 °C and at pH 11. The DNA G+C content was 58.1 mol%. DNA–DNA hybridization results showed values lower than 49 % relatedness with respect to the type strains of the seven closest related species. Therefore, the combined genotypic, phenotypic and chemotaxonomic data support the classification of strain OHA11T to a novel species of the genus Pseudomonas , for which the name Pseudomonas helmanticensis sp. nov. is proposed. The type strain is OHA11T ( = LMG 28168T = CECT 8548T). %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.063560-0