A Gram-reaction-positive, endospore-forming, aerobic bacterium, designated strain CY1T, was isolated from iron mineral soil of Hunan Province, China. The isolate was rod-shaped and motile by means of peritrichous flagella. The major cellular fatty acids were anteiso-C15 : 0 and iso-C16 : 0 and the major quinone was menaquinone 7. The major polar lipids were phosphatidylglycerol and diphosphatidylglycerol phosphatidylethanolamine. The genomic DNA G+C content was 50.5 mol% and the major diagnostic diamino acid in cell-wall peptidoglycan was meso-diaminopimelic acid. Phylogenetic analyses based on the 16S rRNA gene sequence indicated that strain CY1T is most closely related to Paenibacillus chondroitinus DSM 5051T (97.7 % 16S rRNA gene sequence similarity), Paenibacillus pocheonensis Gsoil 1138T (97.4 %) and Paenibacillus frigoriresistens YIM 016T (97.0 %). DNA–DNA hybridization dissociation values were lower than 49 % with the most closely related species. On the basis of phenotypic, chemotaxonomic and phylogenetic evidence, strain CY1T is affiliated to the genus Paenibacillus, but could be distinguished from the species of this genus. A novel species with the name Paenibacillus ferrarius sp. nov. is proposed. The type strain is CY1T ( = KCTC 33419T = CCTCC AB 2013369T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 31170106)
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