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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_9

sp. nov., a moderately halophilic dimorphic prosthecate bacterium isolated from petroleum-contaminated saline soil Free

Abstract

Two novel bacterial strains, SLG210-30A1 and SLG210-19A2, which shared 99.9 % 16S rRNA gene sequence similarity with each other, were isolated from petroleum-contaminated saline soil in Shengli Oilfield, eastern China. Cells were Gram-stain-negative, motile, aerobic, mesophilic and moderately halophilic. They could grow chemoheterotrophically with oxygen as an electron acceptor. Morphologically, cells were typical Caulobacteria-type dimorphic prosthecate bacteria. The genomic DNA G+C contents of strains SLG210-30A1 and SLG210-19A2 were 61.8 mol% and 61.6 mol% respectively. Strain SLG210-30A1 had Q10 as the predominant respiratory ubiquinone, and C (28.4 %), C (11.6 %), C (22.1 %) and Cω7 (14.0 %) as the major cellular fatty acids. The polar lipids of the two isolates were some glycolipids, a lipid, a phospholipid, an aminoglycolipid and an aminophospholipid (all unidentified). The 16S rRNA gene sequences of strains SLG210-30A1 and SLG210-19A2 showed the highest similarities with MCS 33 (99.8–99.9 %), but low sequence similarities (<94.7 %) with type strains of other members of the family . However, the DNA–DNA relatedness of MCS 33 to strains SLG210-30A1 and SLG210-19A2 was 37.4±4.4 % and 36.1±1.1 %, respectively. Based on different physiological, biochemical, and phylogenetic characteristics, strains SLG210-30A1 and SLG210-19A2 represent a novel species of the genus . The name is therefore proposed with strain SLG210-30A1 ( = LMG 27741 = CGMCC 1.12766) as the type strain. An emended description of the genus is also provided.

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31225001)
  • National High Technology Research and Development Program of China (Award 2012AA02A703)
  • Ministry of Land and Resources program: ‘special foundation for scientific research on public causes’ (Award 201111023)
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2014-09-01
2024-04-25
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Glycocaulis albus sp. nov., a moderately halophilic dimorphic prosthecate bacterium isolated from petroleum-contaminated saline soil
Int J Syst Evol Microbiol 64, 3181 (2014); https://doi.org/10.1099/ijs.0.063537-0
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