1887

Abstract

An aerobic, methane-oxidizing bacterium (strain S8) was isolated from marine sediments in Kagoshima Bay, Japan. Phylogenetic analysis based on 16S rRNA gene sequences indicated that this strain is closely related to members of the genus (97.6–97.9 % similarity) within the class . Strain S8 was a Gram-staining-negative, non-motile, coccoid or short rod-shaped organism. The temperature range for growth of strain S8 was 20–47 °C (optimum growth at 36 °C). It required NaCl (>0.5 %), tolerated up to 5 % NaCl and utilized methane and methanol. The major cellular fatty acid and major respiratory quinone were C and 18-methylene ubiquinone 8, respectively. The DNA G+C content was 59.7 mol%. Strain S8 possessed , which encodes soluble methane monooxygenase, as well as , which encodes the particulate methane monooxygenase. On the basis of this morphological, physiological, biochemical and genetic information, the first marine species in the genus is proposed, with the name sp. nov. The type strain is S8 ( = NBRC 109686 = DSM 27392). An emended description of the genus is also provided.

Funding
This study was supported by the:
  • Grant in Aid for Scientific Research on Innovative Areas ‘Genome Science’ (Award 221S0002)
  • Grant-in-Aid for Scientific Research (C) from the Ministry of Education, Culture, Sports, Science and Technology of Japan (Award 225450265)
  • Cooperative Program of the Atmosphere and Ocean Research Institute, The University of Tokyo (Award 005, 2013)
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2014-09-01
2021-10-21
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