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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_10

sp. nov., an endophytic actinomycete isolated from the roots of Free

Abstract

An endophytic actinomycete strain, IXS4, was isolated from the root of , a medicinal plant collected from Yesanpo located in Laishui county, Hebei province, China. The 16S rRNA gene sequence of strain IXS showed most similarity to YIM 61331 (98.23 % 16S rRNA gene sequence similarity), YIM 56256 (98.00 %), E71 (97.90 %) and NRRL B-16327 (97.10 %). DNA–DNA hybridization values between strain IXS4 and the closely related type strains were well below 70 %. The strain also showed a number of physiological and biochemical characteristics that were distinct from the closely related species. The strain contained MK-10(H) and MK-11(H) as the detected menaquinones. The peptidoglycan was mainly -diaminopimelic acid and the whole-cell sugars contained galactose, glucose, mannose, xylose and ribose. The major cellular fatty acids were iso-C, iso-C, iso-C, anteiso-C and anteiso-C. Based on the genetic and phenotypic properties, it is proposed that strain IXS4 represents a novel species of the genus , with the name http://dx.doi.org/10.1601/nm.7671 sp. nov. The type strain is IXS4 ( = HBUM178000 = CGMCC 4.7067 = NBRC 109773).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31270053)
  • Natural Science Foundation of Hebei Province, China (Award C2010000260)
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2014-10-01
2026-01-24

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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J. ( 1990 ). Basic local alignment search tool. . J Mol Biol 215, 403–410. [View Article] [PubMed]
    [Google Scholar]
  2. Bousfield I. J., Keddie R. M., Dando T. R., Shaw S. ( 1985 ). Simple rapid methods of cell wall analysis as an aid in the identification of aerobic coryneform bacteria. . In Chemical Method in Bacterial Systematics, pp. 221–236. Edited by Goodfellow M., Minnikin D. E. . London:: Academic Press;.
     [Google Scholar]
  3. Coombs J. T., Franco C. M. ( 2003 ). Isolation and identification of actinobacteria from surface-sterilized wheat roots. . Appl Environ Microbiol 69, 5603–5608. [View Article] [PubMed]
    [Google Scholar]
  4. Crawford D. L., Lynch J. M., Whipps J. M., Ousley M. A. ( 1993 ). Isolation and characterization of actinomycete antagonists of a fungal root pathogen. . Appl Environ Microbiol 59, 3899–3905.[PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  6. Evtushenko L. I., Taptykova S. D., Akimov V. N., Semyonova S. A., Kalakoutskii L. V. ( 1991 ). Glycomyces tenuis sp. nov.. Int J Syst Bacteriol 41, 154–157. [View Article]
    [Google Scholar]
  7. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  8. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  10. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N. ( 1974 ). Nocardia coeliaca, Nocardia autotrophica, and the nocardin strain. . Int J Syst Bacteriol 24, 54–63. [View Article]
    [Google Scholar]
  11. Gu Q., Zheng W., Huang Y. ( 2007 ). Glycomyces sambucus sp. nov., an endophytic actinomycete isolated from the stem of Sambucus adnata Wall. . Int J Syst Evol Microbiol 57, 1995–1998. [View Article] [PubMed]
    [Google Scholar]
  12. Guan T. W., Xia Z. F., Xiao J., Wu N., Chen Z. J., Zhang L. L., Zhang X. P. ( 2011 ). Glycomyces halotolerans sp. nov., a novel actinomycete isolated from a hypersaline habitat in Xinjiang, China. . Antonie van Leeuwenhoek 100, 137–143. [View Article] [PubMed]
    [Google Scholar]
  13. Hasegawa T., Takizawa M., Tanida S. ( 1983 ). A rapid analysis for chemical grouping of aerobic actinomycetes. . J Gen Appl Microbiol 29, 319–322. [View Article]
    [Google Scholar]
  14. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  15. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. et al. ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  16. Kluge A. G., Farris J. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 1–32. [View Article]
    [Google Scholar]
  17. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  18. Labeda D. P., Kroppenstedt R. M. ( 2004 ). Emended description of the genus Glycomyces and description of Glycomyces algeriensis sp. nov., Glycomyces arizonensis sp. nov. and Glycomyces lechevalierae sp. nov.. Int J Syst Evol Microbiol 54, 2343–2346. [View Article] [PubMed]
    [Google Scholar]
  19. Labeda D. P., Testa R. T., Lechevalier M. P., Lechevalier H. A. ( 1985 ). Glycomyces, a new genus of the Actinomycetales . . Int J Syst Bacteriol 35, 417–421. [View Article]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  21. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  22. Qin S., Wang H. B., Chen H. H., Zhang Y. Q., Jiang C. L., Xu L. H., Li W. J. ( 2008 ). Glycomyces endophyticus sp. nov., an endophytic actinomycete isolated from the root of Carex baccans Nees. . Int J Syst Evol Microbiol 58, 2525–2528. [View Article] [PubMed]
    [Google Scholar]
  23. Qin S., Chen H. H., Klenk H. P., Zhao G. Z., Li J., Xu L. H., Li W. J. ( 2009 ). Glycomyces scopariae sp. nov. and Glycomyces mayteni sp. nov., isolated from medicinal plants in China. . Int J Syst Evol Microbiol 59, 1023–1027. [View Article] [PubMed]
    [Google Scholar]
  24. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  25. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
     [Google Scholar]
  26. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  27. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846–849. [View Article]
    [Google Scholar]
  28. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  29. Waksman S. A. ( 1967 ). The Actinomycetes. A Summary of Current Knowledge. New York:: Ronald Press;.
     [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. et al. ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
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Glycomyces artemisiae sp. nov., an endophytic actinomycete isolated from the roots of Artemisia argyi
Int J Syst Evol Microbiol 64, 3492 (2014); https://doi.org/10.1099/ijs.0.063388-0
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