1887

Abstract

A yellow Gram-stain-positive, non-motile, non-endospore -forming, spherical endophytic actinobacterium, designated strain AE-6, was isolated from the inner fleshy leaf tissues of (Aloe vera) collected from Pune, Maharashtra, India. Strain AE-6 grew at high salt concentrations [10 % (w/v) NaCl], temperatures of 15–41 °C and a pH range of 5–12. It showed highest (99.7 %) 16S rRNA gene sequence similarity with YIM 65004 followed by NCTC 2665 (99.6 %) and YIM 56238 (99.0 %). Ribosomal protein profiling by MALDI-TOF/MS also showed it was most closely related to YIM 65004 and NCTC 2665. Like other members of the genus , strain AE-6 had a high content of branched chain fatty acids (iso-C15 : 0 and anteiso-C15 : 0). MK-8(H) and MK-8 were the predominant isoprenoid quinones. Cell wall analysis showed an ‘A2 -Lys-peptide subunit’ type of peptidoglycan and ribose to be the major cell wall sugar. The DNA G+C content was 70 mol%. Results of DNA–DNA hybridization of AE-6 with its closest relatives from the genus produced a value of less than 70%. Based on the results of this study, strain AE-6 could be clearly differentiated from other members of the genus We propose that it represents a novel species of the genus and suggest the name sp. nov., with strain AE-6 ( = MCC 2184 = DSM 27472) as the type strain of the species.

Funding
This study was supported by the:
  • , Department of Biotechnology , (Award BT/PR/0054/NDB/52/94/2007)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.063339-0
2014-10-01
2020-09-19
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/10/3427.html?itemId=/content/journal/ijsem/10.1099/ijs.0.063339-0&mimeType=html&fmt=ahah

References

  1. Adams P. D., Kloepper J. W. ( 1996 ). Seed-borne bacterial endophytcs in different cotton cultivars. . Phytopathology 86, S97.
    [Google Scholar]
  2. Chen H. H., Zhao G. Z., Park D. J., Zhang Y. Q., Xu L. H., Lee J. C., Kim C. J., Li W. J. ( 2009 ). Micrococcus endophyticus sp. nov., isolated from surface-sterilized Aquilaria sinensis roots. . Int J Syst Evol Microbiol 59, 10701075. [CrossRef] [PubMed]
    [Google Scholar]
  3. Chittpurna, Singh P. K., Verma D., Pinnaka A. K., Mayilraj S., Korpole S. ( 2011 ). Micrococcus lactis sp. nov., isolated from dairy industry waste. . Int J Syst Evol Microbiol 61, 28322836. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cohn F. ( 1872 ). Untersuchungen über Bakterien. . Beitr Biol Pflanz 1, 127±244.
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133142. [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  8. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specified tree topology. . Syst Zool 20, 406416. [CrossRef]
    [Google Scholar]
  9. Gayathri S., Saravanan D., Radhakrishnan M., Balagurunathan R., Kathiresan K. ( 2010 ). Bioprospecting potential of fast growing endophytic bacteria from leaves of mangrove and salt-marsh plant species. . Ind J Biotechnol 9, 397402.
    [Google Scholar]
  10. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184192. [CrossRef] [PubMed]
    [Google Scholar]
  11. James E. K., Gyaneshwar P., Mathan N., Barraquio W. L., Reddy P. M., Iannetta P. P., Olivares F. L., Ladha J. K. ( 2002 ). Infection and colonization of rice seedlings by the plant growth-promoting bacterium Herbaspirillum seropedicae Z67. . Mol Plant Microbe Interact 15, 894906. [CrossRef] [PubMed]
    [Google Scholar]
  12. Johnston-Monje D., Raizada M. N. ( 2011 ). Conservation and diversity of seed associated endophytes in Zea across boundaries of evolution, ethnography and ecology. . PLoS ONE 6, e20396. [CrossRef] [PubMed]
    [Google Scholar]
  13. Kaga H., Mano H., Tanaka F., Watanabe A., Kaneko S., Morisaki H. ( 2009 ). Rice seeds as sources of endophytic bacteria. . Microbes Environ 24, 154162. [CrossRef] [PubMed]
    [Google Scholar]
  14. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [CrossRef] [PubMed]
    [Google Scholar]
  15. Kloos W. E., Tornabene T. G., Schleifer K. H. ( 1974 ). Isolation and characterization of micrococci from human skin, including two new species: Micrococcus lylae and Micrococcus kristinae . . Int J Syst Bacteriol 24, 79101. [CrossRef]
    [Google Scholar]
  16. Liu H., Xu Y., Ma Y., Zhou P. ( 2000 ). Characterization of Micrococcus antarcticus sp. nov., a psychrophilic bacterium from Antarctica. . Int J Syst Evol Microbiol 50, 715719. [CrossRef] [PubMed]
    [Google Scholar]
  17. Liu X. Y., Wang B. J., Jiang C. Y., Liu S. J. ( 2007 ). Micrococcus flavus sp. nov., isolated from activated sludge in a bioreactor. . Int J Syst Evol Microbiol 57, 6669. [CrossRef] [PubMed]
    [Google Scholar]
  18. Loveland-Curtze J., Miteva V. I., Brenchley J. E. ( 2011 ). Evaluation of a new fluorimetric DNA-DNA hybridization method. . Can J Microbiol 57, 250255. [CrossRef] [PubMed]
    [Google Scholar]
  19. Marmur J. ( 1961 ). A procedure for isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [CrossRef]
    [Google Scholar]
  20. Mcinroy J. A., Kloepper J. W. ( 1995 ). Survey of indigenous bacterial endophytes from cotton and sweet corn. . Plant Soil 173, 337342. [CrossRef]
    [Google Scholar]
  21. Prakash O., Lal R. ( 2006 ). Description of Sphingobium fuliginis sp. nov., a phenanthrene-degrading bacterium from a fly ash dumping site, and reclassification of Sphingomonas cloacae as Sphingobium cloacae comb. nov.. Int J Syst Evol Microbiol 56, 21472152. [CrossRef] [PubMed]
    [Google Scholar]
  22. Prakash O., Green S. J., Jasrotia P., Overholt W. A., Canion A., Watson D. B., Brooks S. C., Kostka J. E. ( 2012 ). Rhodanobacter denitrificans sp. nov., isolated from nitrate-rich zones of a contaminated aquifer. . Int J Syst Evol Microbiol 62, 24572462. [CrossRef] [PubMed]
    [Google Scholar]
  23. Prakash O., Munot H., Nimonkar Y., Sharma M., Kumbhare S., Shouche Y. S. ( 2014 ). Description of Pelistega indica sp. nov., isolated from human gut. . Int J Syst Evol Microbiol 64, 13891394. [CrossRef] [PubMed]
    [Google Scholar]
  24. Rieser G., Scherer S., Wenning M. ( 2013 ). Micrococcus cohnii sp. nov., isolated from the air in a medical practice. . Int J Syst Evol Microbiol 63, 8085. [CrossRef] [PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  26. Schumann P. ( 2011 ). Peptidoglycan structure. . Methods Microbiol 38, 101129. [CrossRef]
    [Google Scholar]
  27. Sessitsch A., Reiter B., Pfeifer U., Wilhelm E. ( 2002 ). Cultivation-independent population analysis of bacterial endophytes in three potato varieties based on eubacterial and Actinomycetes-specific PCR of 16S rRNA genes. . FEMS Microbiol Ecol 39, 2332. [CrossRef] [PubMed]
    [Google Scholar]
  28. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  29. Stackebrandt E., Ebers J. ( 2006 ). Taxonomic parameters revisited: tarnished gold standards. . Microbiology Today 33, 152155.
    [Google Scholar]
  30. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic Note: A Place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bact 44, 846849. [CrossRef]
    [Google Scholar]
  31. Stackebrandt E., Koch C., Gvozdiak O., Schumann P. ( 1995 ). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. . Int J Syst Bacteriol 45, 682692. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  34. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128130. [CrossRef]
    [Google Scholar]
  35. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi. . FEMS Microbiol Lett 66, 199202. [CrossRef]
    [Google Scholar]
  36. Verma S., Varma A., Rexer K. H., Hassel A., Kost G., Sarbhoy A., Bisen P., Bütehorn B., Franken P. ( 1998 ). Piriformospora indica, gen. sp. nov., a new root-colonizing fungus. . Mycologia 90, 896903. [CrossRef]
    [Google Scholar]
  37. Waller F., Achatz B., Baltruschat H., Fodor J., Becker K., Fischer M., Heier T., Hückelhoven R., Neumann C. & other authors ( 2005 ). The endophytic fungus Piriformospora indica reprograms barley to salt-stress tolerance, disease resistance, and higher yield. . Proc Natl Acad Sci U S A 102, 1338613391. [CrossRef] [PubMed]
    [Google Scholar]
  38. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [CrossRef]
    [Google Scholar]
  39. Wieser M., Denner E. B. M., Kämpfer P., Schumann P., Tindall B., Steiner U., Vybiral D., Lubitz W., Maszenan A. M. & other authors ( 2002 ). Emended descriptions of the genus Micrococcus, Micrococcus luteus (Cohn 1872) and Micrococcus lylae (Kloos et al. 1974). . Int J Syst Evol Microbiol 52, 629637.[PubMed]
    [Google Scholar]
  40. Xia X. ( 2013 ). dambe5: a comprehensive software package for data analysis in molecular biology and evolution. . Mol Biol Evol 30, 17201728. [CrossRef] [PubMed]
    [Google Scholar]
  41. Zhang J. Y., Liu X. Y., Liu S. J. ( 2010 ). Agrococcus terreus sp. nov. and Micrococcus terreus sp. nov., isolated from forest soil. . Int J Syst Evol Microbiol 60, 18971903. [CrossRef] [PubMed]
    [Google Scholar]
  42. Zhao G. Z., Li J., Qin S., Zhang Y. Q., Zhu W. Y., Jiang C. L., Xu L. H., Li W. J. ( 2009 ). Micrococcus yunnanensis sp. nov., a novel actinobacterium isolated from surface-sterilized Polyspora axillaris roots. . Int J Syst Evol Microbiol 59, 23832387. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.063339-0
Loading
/content/journal/ijsem/10.1099/ijs.0.063339-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error