RT Journal Article SR Electronic(1) A1 Amoozegar, Mohammad Ali A1 Makhdoumi-Kakhki, Ali A1 Mehrshad, Maliheh A1 Riazi, Siavash A1 Ventosa, AntonioYR 2014 T1 Halovivax limisalsi sp. nov., an extremely halophilic archaeon from hypersaline mud JF International Journal of Systematic and Evolutionary Microbiology, VO 64 IS Pt_10 SP 3422 OP 3426 DO https://doi.org/10.1099/ijs.0.063297-0 PB Microbiology Society, SN 1466-5034, AB A Gram-stain-negative, cream‐pigmented, motile, extremely halophilic archaeon, designated strain IC38T, was isolated from a saline mud sample taken from a hypersaline lake, Aran-Bidgol, in Iran. The strain required at least 2.5 M NaCl for growth. However, MgCl2 was not required. Optimal growth occurred with 4.3 M NaCl and 0.2 M MgCl2. The optimum pH and temperature for growth were pH 7.0 and 35 °C, respectively, and strain IC38T was able to grow over a pH range of 6.5–9.0, and a temperature range of 25–45 °C. Analysis of the 16S rRNA gene sequence revealed that strain IC38T clustered with the two species of the genus Halovivax , Halovivax asiaticus EJ-46T and Halovivax ruber XH-70T, with sequence similarities of 96.4 % and 96.1 %, respectively. The similarities between the rpoB′ gene of the novel strain and Halovivax asiaticus and Halovivax ruber were 90.7 % and 90.3 %, respectively. The polar lipid pattern of strain IC38T consisted of phosphatidylglycerol and phosphatidylglycerol phosphate methyl ester. Three unidentified glycolipids and two minor phospholipids were also observed. The DNA G+C content of strain IC38T was 62.6 mol%. On the basis of the phylogenetic analysis, as well as the biochemical and physiological characteristics, the new isolate is suggested to be a representative of a novel species of the genus Halovivax , for which the name Halovivax limisalsi sp. nov. is proposed. The type strain of Halovivax limisalsi is IC38T ( = IBRC-M 10022T = KCTC 4051T)., UL https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.063297-0