1887

Abstract

Four Gram-stain-positive, catalase-negative, coccoid-shaped organisms were isolated from elephant oral cavities. The isolates were tentatively identified as streptococcal species based on the results of biochemical tests. Comparative 16S rRNA gene sequencing studies confirmed the organisms to be members of the genus . Two isolates (NUM 6304 and NUM 6312) were related most closely to with 96.8 % and 93.1 % similarity based on the 16S rRNA gene and the RNA polymerase β subunit encoding gene (), respectively, and to with 83.7 % similarity based on the 60 kDa heat-shock protein gene (). The other two isolates (NUM 6306 and NUM 6318) were related most closely to with 97.0 % and 82.9 % similarity based on the 16S rRNA and genes, respectively, and to with 93.5 % similarity based on the gene. Based on phylogenetic and phenotypic evidence, these isolates are suggested to represent novel species of the genus , for which the names sp. nov. (type strain NUM 6304 = JCM 19287 = DSM 27382) and sp. nov. (type strain NUM 6306 = JCM 19288 = DSM 27513) are proposed.

Funding
This study was supported by the:
  • JSPS KAKENHI (Award 25870768)
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2014-09-01
2022-01-19
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