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Abstract

The Gram-stain-negative, rod-shaped bacterial isolate BT-1 is the closest relative to the genus ‘ ’ cultured to date. BT-1 was recovered from the phloem sap of a defoliating mountain papaya in Puerto Rico. The BT-1 16S rRNA gene sequence showed that strain BT-1 is most closely related to members of the genus ‘ ’ sharing 94.7 % 16S rRNA gene sequence similarity with ‘ ’ and ‘ ’. Additionally, average nucleotide identity, 16S rRNA gene sequences and conserved protein sequences supported inclusion of the previously described species of the genus ‘ ’ in a genus with BT-1. The prominent fatty acids of isolate BT-1 were Cω7 (77.2 %), C OH (4.8 %), C (4.4 %) and C (3.5 %). Both physiological and genomic characteristics support the creation of the genus , as well as the novel species gen. nov., sp. nov. with type strain BT-1 ( = ATCC BAA-2481 = DSM 26877).

Funding
This study was supported by the:
  • Citrus Research and Development Foundation (Award 769, 767 and 336)
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2014-07-01
2024-12-13
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References

  1. Alsmark C. M., Frank A. C., Karlberg E. O., Legault B. A., Ardell D. H., Canbäck B., Eriksson A. S., Näslund A. K., Handley S. A. & other authors ( 2004 ). The louse-borne human pathogen Bartonella quintana is a genomic derivative of the zoonotic agent Bartonella henselae . . Proc Natl Acad Sci U S A 101, 97169721. [View Article] [PubMed]
    [Google Scholar]
  2. Anisimova M., Gascuel O. ( 2006 ). Approximate likelihood-ratio test for branches: a fast, accurate, and powerful alternative. . Syst Biol 55, 539552. [View Article] [PubMed]
    [Google Scholar]
  3. Brown P. J. B., de Pedro M. A., Kysela D. T., Van der Henst C., Kim J., De Bolle X., Fuqua C., Brun Y. V. ( 2012 ). Polar growth in the Alphaproteobacterial order Rhizobiales . . Proc Natl Acad Sci U S A 109, 16971701. [View Article] [PubMed]
    [Google Scholar]
  4. Capoor S. P., Rao D. G., Viswanath S. M. ( 1967 ). Diaphorina citri kuway, a vector of greening disease of citrus in India. . Indian J Agric Sci 37, 572577.
    [Google Scholar]
  5. Castresana J. ( 2000 ). Selection of conserved blocks from multiple alignments for their use in phylogenetic analysis. . Mol Biol Evol 17, 540552. [View Article] [PubMed]
    [Google Scholar]
  6. Chenoweth M. R., Somerville G. A., Krause D. C., O’Reilly K. L., Gherardini F. C. ( 2004 ). Growth characteristics of Bartonella henselae in a novel liquid medium: primary isolation, growth-phase-dependent phage induction, and metabolic studies. . Appl Environ Microbiol 70, 656663. [View Article] [PubMed]
    [Google Scholar]
  7. Chevenet F., Brun C., Bañuls A. L., Jacq B., Christen R. ( 2006 ). TreeDyn: towards dynamic graphics and annotations for analyses of trees. . BMC Bioinformatics 7, 439. [View Article] [PubMed]
    [Google Scholar]
  8. Davis M. J., Mondal S. N., Chen H., Rogers M. E., Brlansky R. H. ( 2008 ). Co-cultivation of ‘Candidatus Liberibacter asiaticus’ with Actinobacteria from citrus with Huanglongbing. . Plant Dis 92, 15471550. [View Article]
    [Google Scholar]
  9. Dereeper A., Guignon V., Blanc G., Audic S., Buffet S., Chevenet F., Dufayard J. F., Guindon S., Lefort V. & other authors ( 2008 ). Phylogeny.fr: robust phylogenetic analysis for the non-specialist. . Nucleic Acids Res 36 (Web Server), W465W469. [View Article] [PubMed]
    [Google Scholar]
  10. Edgar R. C. ( 2004 ). muscle: multiple sequence alignment with high accuracy and high throughput. . Nucleic Acids Res 32, 17921797. [View Article] [PubMed]
    [Google Scholar]
  11. Fagen J. R., Leonard M. T., McCullough C. M., Edirisinghe J. N., Henry C. S., Davis M. J., Triplett E. W. ( 2014 ). Comparative genomics of cultured and uncultured strains suggests genes essential for free-living growth of Liberibacter . . PLoS ONE 9, e84469. [View Article] [PubMed]
    [Google Scholar]
  12. Galibert F., Finan T. M., Long S. R., Puhler A., Abola P., Ampe F., Barloy-Hubler F., Barnett M. J., Becker A. & other authors ( 2001 ). The composite genome of the legume symbiont Sinorhizobium meliloti . . Science 293, 668672. [View Article] [PubMed]
    [Google Scholar]
  13. Guindon S., Gascuel O. ( 2003 ). A simple, fast, and accurate algorithm to estimate large phylogenies by maximum likelihood. . Syst Biol 52, 696704. [View Article] [PubMed]
    [Google Scholar]
  14. Hansen A. K., Trumble J. T., Stouthamer R., Paine T. D. ( 2008 ). A new Huanglongbing Species, “Candidatus Liberibacter psyllaurous,” found to infect tomato and potato, is vectored by the psyllid Bactericera cockerelli (Sulc). . Appl Environ Microbiol 74, 58625865. [View Article] [PubMed]
    [Google Scholar]
  15. Hertig M. ( 1936 ). The rickettsia, Wolbachia pipientis (genet sp. n.) and associated inclusions of the mosquito Culex pipiens . . Parasitology 28, 453486. [View Article]
    [Google Scholar]
  16. Jagoueix S., Bove J. M., Garnier M. ( 1994 ). The phloem-limited bacterium of greening disease of citrus is a member of the α subdivision of the Proteobacteria . . Int J Syst Bacteriol 44, 379386. [View Article] [PubMed]
    [Google Scholar]
  17. Jarvis B. D. W., Tighe S. W. ( 1994 ). Rapid identification of Rhizobium species based on cellular fatty acid analysis. . Plant Soil 161, 3141. [View Article]
    [Google Scholar]
  18. Kapur S. P., Kapoor S. K., Cheema S. S., Dhillon R. S. ( 1978 ). Effect of greening disease on tree and fruit characters of Kinnow mandarin. . Punjab Hort J 50, 7679.
    [Google Scholar]
  19. Leonard M. T., Fagen J. R., Davis-Richardson A. G., Davis M. J., Triplett E. W. ( 2012 ). Complete genome sequence of Liberibacter crescens BT-1. . Stand Genomic Sci 7, 271283. [View Article] [PubMed]
    [Google Scholar]
  20. Liefting L. W., Weir B. S., Pennycook S. R., Clover G. R. G. ( 2009 ). Candidatus Liberibacter solanacearum’, associated with plants in the family Solanaceae. . Int J Syst Evol Microbiol 59, 22742276. [View Article] [PubMed]
    [Google Scholar]
  21. Murray R. G. E., Stackebrandt E. ( 1995 ). Taxonomic note: implementation of the provisional status Candidatus for incompletely described procaryotes. . Int J Syst Bacteriol 45, 186187. [View Article] [PubMed]
    [Google Scholar]
  22. Pelz-Stelinski K. S., Brlansky R. H., Ebert T. A., Rogers M. E. ( 2010 ). Transmission parameters for Candidatus liberibacter asiaticus by Asian citrus psyllid (Hemiptera: Psyllidae). . J Econ Entomol 103, 15311541. [View Article] [PubMed]
    [Google Scholar]
  23. Pérez K. A., Piñol B., Rosete Y. A., Wilson M., Boa E., Lucas J. ( 2010 ). Transmission of the phytoplasma associated with bunchy top symptom of papaya by Empoasca papaya Oman. . J Phytopathol 158, 194196. [View Article]
    [Google Scholar]
  24. Raddadi N., Gonella E., Camerota C., Pizzinat A., Tedeschi R., Crotti E., Mandrioli M., Bianco P. A., Daffonchio D., Alma A. ( 2011 ). Candidatus Liberibacter europaeus’ sp. nov. that is associated with and transmitted by the psyllid Cacopsylla pyri apparently behaves as an endophyte rather than a pathogen. . Environ Microbiol 13, 414426. [View Article] [PubMed]
    [Google Scholar]
  25. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 1912619131. [View Article] [PubMed]
    [Google Scholar]
  26. Segovia L., Young J. P. W., Martínez-Romero E. ( 1993 ). Reclassification of American Rhizobium leguminosarum biovar phaseoli type I strains as Rhizobium etli sp. nov.. Int J Syst Bacteriol 43, 374377. [View Article] [PubMed]
    [Google Scholar]
  27. Shokrollah H., Abdullah T. L., Sijam K., Abdullah S. N. A. ( 2010 ). Ultrastructure of Candidatus Liberibacter asiaticus and its damage in Huanglongbing (HLB) infected citrus. . African J Biotechnol 9, 58975901.
    [Google Scholar]
  28. Stamatakis A. ( 2006 ). RAxML-VI-HPC: maximum likelihood-based phylogenetic analyses with thousands of taxa and mixed models. . Bioinformatics 22, 26882690. [View Article] [PubMed]
    [Google Scholar]
  29. Teixeira D. C., Saillard C., Eveillard S., Danet J. L., da Costa P. I., Ayres A. J., Bové J. ( 2005 ). Candidatus Liberibacter americanus’, associated with citrus huanglongbing (greening disease) in São Paulo State, Brazil. . Int J Syst Evol Microbiol 55, 18571862. [View Article] [PubMed]
    [Google Scholar]
  30. Welch D. F., Pickett D. A., Slater L. N., Steigerwalt A. G., Brenner D. J. ( 1992 ). Rochalimaea henselae sp. nov., a cause of septicemia, bacillary angiomatosis, and parenchymal bacillary peliosis. . J Clin Microbiol 30, 275280.[PubMed]
    [Google Scholar]
  31. Werren J. H. ( 1997 ). Biology of Wolbachia . . Annu Rev Entomol 42, 587609. [View Article] [PubMed]
    [Google Scholar]
  32. Zhang S., Flores-Cruz Z., Zhou L., Kang B.-H., Fleites L. A., Gooch M. D., Wulff N. A., Davis M. J., Duan Y.-P., Gabriel D. W. ( 2011 ). Ca. Liberibacter asiaticus’ carries an excision plasmid prophage and a chromosomally integrated prophage that becomes lytic in plant infections. . Mol Plant Microbe Interact 24, 458468. [View Article] [PubMed]
    [Google Scholar]
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