1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_10

sp. nov., a novel arsenic-resistant bacterium from high-arsenic sediment Free

Abstract

A novel bacterial strain Z was isolated from the high-arsenic sediment in Jianghan Plain, China. The strain was Gram-staining-negative, rod-shaped and formed yellow colonies. This bacterium is capable of tolerating arsenate and arsenite, with MICs of 40 mM and 20 mM, respectively. The strain also possesses catalase and does not produce oxidase. The nucleotide sequence of the 16S rRNA gene of the isolate showed the highest similarity (96.9 %) to that of the type strain of . On the basis of the 16S rRNA gene sequence analysis and the phenotypic properties of strain Z, it was assigned to the genus . The major respiratory menaquinone was MK-6 and the predominant fatty acids were iso-C, summed feature 3 (containing Cω6 and/or Cω7) and iso-CG. The major polar lipids were phosphatidylethanolamine, three uncharacterized aminophospholipids and four unidentified phospholipids. The DNA G+C content was 32.1 mol%. Based on the phenotypic and genotypic data presented in this article, it can be concluded that this isolate represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is Z ( = CCTCC AB 2013048 = KCTC 32397).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41072181 and 41272257)
  • Fund for the Faculty of State Key Laboratory of Biogeology and Environmental Geology (BGEG) (Award GBL11208)
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.063248-0
2014-10-01
2024-04-24
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/10/3369.html?itemId=/content/journal/ijsem/10.1099/ijs.0.063248-0&mimeType=html&fmt=ahah

References

  1. Bergey D. H., Harrison F. C., Breeds R. S., Hammer B. W., Huntoon F. M. (editors) ( 1923 ). Genus II. Flavobacterium gen. nov.. In Bergey's Manual of Determinative Bacteriology, pp. 97–117. Baltimore:: Williams & Wilkins;.
     [Google Scholar]
  2. Bernardet J. F., Bowman J. P. ( 2006 ). The genus Flavobacterium . . In The Prokaryotes: A Handbook on the Biology of Bacteria, , 3rd edn., vol. 7, pp. 481–531. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York, NY:: Springer;. [View Article]
    [Google Scholar]
  3. Bernardet J. F., Bowman J. P. ( 2011 ). Genus I. Flavobacterium Bergey et al. 1923. . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 112–154. Edited by Whitman W. . Baltimore:: The Williams & Wilkins;.
     [Google Scholar]
  4. Bernardet J. F., Segers P., Vancanneyt M., Berthe F., Kersters K., Vandamme P. ( 1996 ). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (Basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol 46, 128–148. [View Article]
    [Google Scholar]
  5. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  6. Chen W. M., Huang W. C., Young C. C., Sheu S. Y. ( 2013 ). Flavobacterium tilapiae sp. nov., isolated from a freshwater pond, and emended descriptions of Flavobacterium defluvii and Flavobacterium johnsoniae . . Int J Syst Evol Microbiol 63, 827–834. [View Article] [PubMed]
    [Google Scholar]
  7. Dong K., Xu B., Zhu F. Q., Wang G. J. ( 2013 ). Flavobacterium hauense sp. nov., isolated from soil and emended descriptions of Flavobacterium subsaxonicum, Flavobacterium beibuense and Flavobacterium rivuli . . Int J Syst Evol Microbiol 63, 3237–3242. [View Article] [PubMed]
    [Google Scholar]
  8. Fendorf S., Michael H. A., van Geen A. ( 2010 ). Spatial and temporal variations of groundwater arsenic in South and Southeast Asia. . Science 328, 1123–1127. [View Article] [PubMed]
    [Google Scholar]
  9. Fu Y., Tang X., Lai Q., Zhang C., Zhong H., Li W., Liu Y., Chen L., Sun F., Shao Z. ( 2011 ). Flavobacterium beibuense sp. nov., isolated from marine sediment. . Int J Syst Evol Microbiol 61, 205–209. [View Article] [PubMed]
    [Google Scholar]
  10. Glaeser S. P., Galatis H., Martin K., Kämpfer P. ( 2013 ). Flavobacterium cutihirudinis sp. nov., isolated from Hirudo verbana . . Int J Syst Evol Microbiol 63, 2841–2847. [View Article] [PubMed]
    [Google Scholar]
  11. Huang S. B., Liu C. R., Wang Y. X., Zhan H. B. ( 2014 ). Multivariate analysis of the heterogeneous geochemical processes controlling arsenic enrichment in a shallow groundwater system. . J Environ Sci Health A Tox Hazard Subst Environ Eng 49, 478–489. [View Article] [PubMed]
    [Google Scholar]
  12. Joung Y., Kim H., Ahn T. S., Joh K. ( 2012 ). Flavobacterium yonginense sp. nov. and Flavobacterium myungsuense sp. nov., isolated from a mesotrophic artificial lake. . Int J Syst Evol Microbiol 62, 806–810. [View Article] [PubMed]
    [Google Scholar]
  13. Kämpfer P., Lodders N., Martin K., Avendaño-Herrera R. ( 2012 ). Flavobacterium chilense sp. nov. and Flavobacterium araucananum sp. nov., isolated from farmed salmonid fish. . Int J Syst Evol Microbiol 62, 1402–1408. [View Article] [PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  15. Kim Y. J., Kim S. R., Nguyen N. L., Yang D. C. ( 2013 ). Flavobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 63, 4289–4293. [View Article] [PubMed]
    [Google Scholar]
  16. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161–207. [View Article]
    [Google Scholar]
  17. Lane D. J. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–176. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
     [Google Scholar]
  18. Lee S., Weon H.-Y., Han K., Ahn T.-Y. ( 2012 ). Flavobacterium dankookense sp. nov., isolated from a freshwater reservoir, and emended descriptions of Flavobacterium cheonanense, F. chungnamense, F. koreense and F. aquatile . . Int J Syst Evol Microbiol 62, 2378–2382. [View Article] [PubMed]
    [Google Scholar]
  19. Li A., Liu H., Sun B., Zhou Y., Xin Y. ( 2014 ). Flavobacterium lacus sp. nov., isolated from a high-altitude lake, and emended description of Flavobacterium filum . . Int J Syst Evol Microbiol 64, 933–939. [View Article] [PubMed]
    [Google Scholar]
  20. Liu H., Liu R., Yang S. Y., Gao W. K., Zhang C. X., Zhang K. Y., Lai R. ( 2008 ). Flavobacterium anhuiense sp. nov., isolated from field soil. . Int J Syst Evol Microbiol 58, 756–760. [View Article] [PubMed]
    [Google Scholar]
  21. Liu Y., Jin J. H., Zhou Y. G., Liu H. C., Liu Z. P. ( 2010 ). Flavobacterium caeni sp. nov., isolated from a sequencing batch reactor for the treatment of malachite green effluents. . Int J Syst Evol Microbiol 60, 417–421. [View Article] [PubMed]
    [Google Scholar]
  22. Ludwig W., Euzéby J., Whitman W. B. ( 2011 ). Taxonomic outlines of the phyla Bacteroidetes, Spirochaetes, Tenericutes (Mollicutes), Acidobacteria, Fibrobacteres, Fusobacteria, Dictyoglomi, Gemmatimonadetes, Lentisphaerae, Verrucomicrobia, Chlamydiae, and Planctomycetes . . In Bergey's Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 21–24. Edited by Whitman W. . Baltimore:: The Williams & Wilkins Co;.
     [Google Scholar]
  23. McCammon S. A., Bowman J. P. ( 2000 ). Taxonomy of Antarctic Flavobacterium species: description of Flavobacterium gillisiae sp. nov., Flavobacterium tegetincola sp. nov., and Flavobacterium xanthum sp. nov., nom. rev. and reclassification of [Flavobacterium] salegens as Salegentibacter salegens gen. nov., comb. nov.. Int J Syst Evol Microbiol 50, 1055–1063. [View Article] [PubMed]
    [Google Scholar]
  24. Mesbah M., Premachandran U., Whitman W. ( 1989 ). Precise measurement of the G+C content of desoxyribonucleic acids by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  25. Minnikin D. E., O'Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  26. Moore D. D., Dowhan D. ( 1995 ). Preparation and analysis of DNA. . In Current Protocols in Molecular Biology, pp. 2–11. Edited by Ausubel F. W., Brent R., Kingston R. E., Moore D. D., Seidman J. G., Smith J. A., Struhl K. . New York:: Wiley;.
     [Google Scholar]
  27. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  28. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhart P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  29. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  30. Van Trappen S., Mergaert J., Swings J. ( 2003 ). Flavobacterium gelidilacus sp. nov., isolated from microbial mats in Antarctic lakes. . Int J Syst Evol Microbiol 53, 1241–1245. [View Article] [PubMed]
    [Google Scholar]
  31. Wang Z. W., Liu Y. H., Dai X., Wang B. J., Jiang C. Y., Liu S. J. ( 2006 ). Flavobacterium saliperosum sp. nov., isolated from freshwater lake sediment. . Int J Syst Evol Microbiol 56, 439–442. [View Article] [PubMed]
    [Google Scholar]
  32. Yang J. E., Kim S. Y., Im W. T., Yi T. H. ( 2011 ). Flavobacterium ginsenosidimutans sp. nov., a bacterium with ginsenoside converting activity isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 61, 1408–1412. [View Article] [PubMed]
    [Google Scholar]
  33. Yi H., Chun J. ( 2006 ). Flavobacterium weaverense sp. nov. and Flavobacterium segetis sp. nov., novel psychrophiles isolated from the Antarctic. . Int J Syst Evol Microbiol 56, 1239–1244. [View Article] [PubMed]
    [Google Scholar]
  34. Yoon J. H., Kang S. J., Oh T. K. ( 2006 ). Flavobacterium soli sp. nov., isolated from soil. . Int J Syst Evol Microbiol 56, 997–1000. [View Article] [PubMed]
    [Google Scholar]
  35. Yoon J. H., Park S., Kang S. J., Oh S. J., Myung S. C., Kim W. ( 2011 ). Flavobacterium ponti sp. nov., isolated from seawater. . Int J Syst Evol Microbiol 61, 81–85. [View Article] [PubMed]
    [Google Scholar]
  36. Zamora L., Vela A. I., Sánchez-Porro C., Palacios M. A., Moore E. R., Domínguez L., Ventosa A., Fernández-Garayzábal J. F. ( 2014 ). Flavobacterium tructae sp. nov. and Flavobacterium piscis sp. nov., isolated from farmed rainbow trout (Oncorhynchus mykiss). . Int J Syst Evol Microbiol 64, 392–399. [View Article] [PubMed]
    [Google Scholar]
  37. Zhu L., Liu Q., Liu H. C., Zhang J. L., Dong X. Z., Zhou Y. G., Xin Y. H. ( 2013 ). Flavobacterium noncentrifugens sp. nov., a psychrotolerant bacterium isolated from glacier meltwater. . Int J Syst Evol Microbiol 63, 2032–2037. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.063248-0
Loading
Flavobacterium arsenatis sp. nov., a novel arsenic-resistant bacterium from high-arsenic sediment
Int J Syst Evol Microbiol 64, 3369 (2014); https://doi.org/10.1099/ijs.0.063248-0
/content/journal/ijsem/10.1099/ijs.0.063248-0
/content/journal/ijsem/10.1099/ijs.0.063248-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error