1887

Abstract

Biochemical and molecular genetic studies were performed on four unknown Gram-stain-positive, catalase-negative, coccus-shaped organisms isolated from tonsils ( = 3) and nasal samples ( = 1) of four wild rabbits. The micro-organism was identified as a streptococcal species based on its cellular morphological and biochemical tests. Comparative 16S rRNA gene sequencing confirmed its identification as a member of the genus , but the organism did not correspond to any recognized species of this genus. The closest phylogenetic relative of the unknown cocci from wild rabbits was NCIMB 702025 (97.9 % 16S rRNA gene sequence similarity). and sequence analysis of the novel isolate showed interspecies divergence of 16.2 % and 20.3 %, respectively, from the type strain of its closest 16S rRNA gene phylogenetic relative, . The novel bacterial isolate could be distinguished from the type strain of by several biochemical characteristics, such as the production of esterase C4, acid phosphatase and naphthol-AS-BI-phosphohydrolase and acidification of different sugars. Based on both phenotypic and phylogenetic findings, it is proposed that the unknown bacterium be classified as a novel species of the genus , sp. nov. The type strain is NED12-00049-6B ( = CECT 8498 = CCUG 65085).

Funding
This study was supported by the:
  • Spanish Ministry of Education and Science (MEC) (Award AGL2009-14303-C02-01)
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2014-07-01
2021-10-21
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