Description of Chishuiella changwenlii gen. nov., sp. nov., isolated from freshwater, and transfer of Wautersiella falsenii to the genus Empedobacter as Empedobacterfalsenii comb. nov.
A Gram-reaction-negative, strictly aerobic, non-pigmented, non-gliding, rod-shaped bacterium, designated strain BY4T, was isolated from freshwater. Cells were catalase- and oxidase-positive and indole was produced. Phylogenetic analyses based on 16S rRNA gene sequences indicated that strain BY4T belonged to the family Flavobacteriaceae and showed 91.6–95.9 % sequence similarities to the most closely related strains. The major respiratory quinone was MK-6 and the major polar lipid was phosphatidylethanolamine. The major polyamine was homospermidine and the major fatty acids were iso-C15 : 0, iso-C17 : 0 3-OH and summed feature 3 (C16 : 1ω7c and/or C16 : 1ω6c). The DNA G+C content was 30.0 mol%. On the basis of phenotypic, phylogenetic and genotypic features, strain BY4T is suggested to represent a novel species in a new genus within the family Flavobacteriaceae, for which the name Chishuiella changwenlii gen. nov., sp. nov. is proposed. The type strain of this type species is BY4T ( = CGMCC 1.12707T = JCM 19633T). On the basis of data collected from previous and present studies, it is proposed to reclassify Wautersiella falsenii to the genus Empedobacter as the new combination Empedobacter falsenii comb. nov. (type strain NF 993T = CCUG 51536T = CIP 108861T).
BernardetJ.-F.(2011). Family I. Flavobacteriaceae Reichenbach 1992. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 4, pp. 106–111. Edited by
KriegN. R.,
LudwigW.,
WhitmanW. B.,
HedlundB. P.,
PasterB. J.,
StaleyJ. T.,
WardN. L.,
BrownD. R.,
ParteA.
. New York:: Springer;.
BernardetJ.-F.,
SegersP.,
VancanneytM.,
BertheF.,
KerstersK.,
VandammeP.(1996). Cutting a Gordian knot: emended classification and description of the genus Flavobacterium, emended description of the family Flavobacteriaceae, and proposal of Flavobacterium hydatis nom. nov. (basonym, Cytophaga aquatilis Strohl and Tait 1978). . Int J Syst Bacteriol46, 128–148. [View Article]
BernardetJ.-F.,
NakagawaY.,
HolmesB.Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes(2002). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol52, 1049–1070. [View Article][PubMed]
GarrityG. M.,
HoltJ. G.(2001). The road map to the Manual. . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 1, pp. 119–166. Edited by
BooneD. R.,
CastenholzR. W.,
GarrityG. M.
. New York:: Springer;. [View Article]
GonzalezJ. M.,
Saiz-JimenezC.(2005). A simple fluorimetric method for the estimation of DNA–DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles9, 75–79. [View Article][PubMed]
HamanaK.,
NakagawaY.(2001). Polyamine distribution profiles in the eighteen genera phylogenetically located within the Flavobacterium–Flexibacter–Cytophaga complex. . Microbios106, 7–17.[PubMed]
HolmesB.,
SnellJ. J. S.,
LapageS. P.(1978). Revised description, from clinical strains, of Flavobacterium breve (Lustig) Bergey et al. 1923 and proposal of the neotype strain. . Int J Syst Bacteriol28, 201–208. [View Article]
HolmesB.,
SteigerwaltA. G.,
WeaverR. E.,
BrennerD. J.(1986).Weeksella virosa gen. nov., sp. nov. (formerly group IIf), found in human clinical specimens. . Syst Appl Microbiol8, 185–190. [View Article]
KämpferP.,
AvesaniV.,
JanssensM.,
CharlierJ.,
De BaereT.,
VaneechoutteM.(2006). Description of Wautersiella falsenii gen. nov., sp. nov., to accommodate clinical isolates phenotypically resembling members of the genera Chryseobacterium and Empedobacter
. . Int J Syst Evol Microbiol56, 2323–2329. [View Article][PubMed]
ReichenbachH.(1989). Order I. Cytophagales Leadbetter 1974, 99AL
. . In Bergey’s Manual of Systematic Bacteriology, vol. 3, pp. 2011–2082. Edited by
StaleyJ. T.,
BryantM. P.,
PfennigN.,
HoltJ. G.
. Baltimore, MD:: Williams & Wilkins;.
VandammeP.,
BernardetJ.-F.,
SegersP.,
KerstersK.,
HolmesB.(1994). New perspectives in the classification of the flavobacteria: description of Chryseobacterium gen. nov., Bergeyella gen. nov., and Empedobacter nom. rev.. Int J Syst Bacteriol44, 827–831. [View Article]
VaneechoutteM.,
De BeenhouwerH.,
ClaeysG.,
VerschraegenG.,
De RouckA.,
PaepeN.,
ElaichouniA.,
PortaelsF.(1993). Identification of Mycobacterium species by using amplified ribosomal DNA restriction analysis. . J Clin Microbiol31, 2061–2065.[PubMed]
WangQ.,
GarrityG. M.,
TiedjeJ. M.,
ColeJ. R.(2007). Naive Bayesian classifier for rapid assignment of rRNA sequences into the new bacterial taxonomy. . Appl Environ Microbiol73, 5261–5267. [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW.,
MurrayR.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]
ZhangR. G.,
TanX.,
ZhaoX. M.,
DengJ.,
LvJ.(2014).Moheibacter sediminis gen. nov., sp. nov., a member of the family Flavobacteriaceae isolated from sediment, and emended descriptions of Empedobacter brevis, Wautersiella falsenii and Weeksella virosa
. . Int J Syst Evol Microbiol64, 1481–1487. [View Article][PubMed]
Description of Chishuiella changwenlii gen. nov., sp. nov., isolated from freshwater, and transfer of Wautersiella falsenii to the genus Empedobacter as Empedobacterfalsenii comb. nov.