1887

Abstract

A bacterial strain, designated 1P10ME, which was resistant to extreme doses of ionizing radiation, pale-pink, non-motile, and a tetrad-forming coccoid was isolated from a cleanroom at the Kennedy Space Center, where the Phoenix spacecraft was assembled. Strain 1P10ME showed optimum growth at 30 °C, with a pH range for growth of 6.5–9.0 and was highly sensitive to sodium chloride, growing only in medium with no added NaCl. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain 1P10ME represents a novel member of the genus , with low sequence similarities (<93.5 %) to recognized species of the genus . The predominant cellular fatty acid was C : 1ω6. This novel strain exhibits extreme resistance to gamma radiation (D >8 kGy) and UV (D >1000 Jm). The results of our polyphasic taxonomic analyses suggest that strain 1P10ME represents a novel species of the genus , for which the name Deinococcus sp. nov. is proposed. The type strain is 1P10ME ( = NRRL B-59546 = DSM 27173).

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2014-10-01
2019-09-16
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References

  1. Cole R. M., Popkin T. J.. ( 1981;). Electron microscopy. . In Manual of Methods for General Bacteriology, pp. 34–51. Edited by Gerhardt P., Murray R. G. E., Costilaw R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B... Washington, D.C.:: American Society for Microbiology;.
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  3. Cox M. M., Battista J. R.. ( 2005;). Deinococcus radiodurans - the consummate survivor. . Nat Rev Microbiol 3:, 882–892. [CrossRef][PubMed]
    [Google Scholar]
  4. Crawford R. L.. ( 2005;). Microbial diversity and its relationship to planetary protection. . Appl Environ Microbiol 71:, 4163–4168. [CrossRef][PubMed]
    [Google Scholar]
  5. de Groot A., Chapon V., Servant P., Christen R., Saux M. F., Sommer S., Heulin T.. ( 2005;). Deinococcus deserti sp. nov., a gamma-radiation-tolerant bacterium isolated from the Sahara Desert. . Int J Syst Evol Microbiol 55:, 2441–2446. [CrossRef][PubMed]
    [Google Scholar]
  6. Ferreira A. C., Nobre M. F., Rainey F. A., Silva M. T., Wait R., Burghardt J., Chung A. P., da Costa M. S.. ( 1997;). Deinococcus geothermalis sp. nov. and Deinococcus murrayi sp. nov., two extremely radiation-resistant and slightly thermophilic species from hot springs. . Int J Syst Bacteriol 47:, 939–947. [CrossRef][PubMed]
    [Google Scholar]
  7. Ghosh S., Osman S., Vaishampayan P., Venkateswaran K.. ( 2010;). Recurrent isolation of extremotolerant bacteria from the clean room where Phoenix spacecraft components were assembled. . Astrobiology 10:, 325–335. [CrossRef][PubMed]
    [Google Scholar]
  8. Johnson J. L.. ( 1981;). Genetic characterization. . In Manual of Methods for General Bacteriology, pp. 450–472 Edited by Gerhardt P., Murray R. G. E., Costilaw R. N., Nester E. W., Wood W. A., Krieg N. R., Phillips G. B... Washington, D.C:: American Society for Microbiology;.
    [Google Scholar]
  9. Kempf M. J., Chen F., Kern R., Venkateswaran K.. ( 2005;). Recurrent isolation of hydrogen peroxide-resistant spores of Bacillus pumilus from a spacecraft assembly facility. . Astrobiology 5:, 391–405. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  11. La Duc M. T., Nicholson W., Kern R., Venkateswaran K.. ( 2003;). Microbial characterization of the Mars Odyssey spacecraft and its encapsulation facility. . Environ Microbiol 5:, 977–985. [CrossRef][PubMed]
    [Google Scholar]
  12. La Duc M. T., Satomi M., Venkateswaran K.. ( 2004;). Bacillus odysseyi sp. nov., a round-spore-forming bacillus isolated from the Mars Odyssey spacecraft. . Int J Syst Evol Microbiol 54:, 195–201. [CrossRef][PubMed]
    [Google Scholar]
  13. La Duc M. T., Dekas A. E., Osman S., Moissl C., Newcombe D., Venkateswaran K.. ( 2007;). Isolation and characterization of bacteria capable of tolerating the extreme conditions of clean room environments. . Appl Environ Microbiol 73:, 2600–2611. [CrossRef][PubMed]
    [Google Scholar]
  14. Lai W. A., Kämpfer P., Arun A. B., Shen F. T., Huber B., Rekha P. D., Young C. C.. ( 2006;). Deinococcus ficus sp. nov., isolated from the rhizosphere of Ficus religiosa L. . Int J Syst Evol Microbiol 56:, 787–791. [CrossRef][PubMed]
    [Google Scholar]
  15. Mattimore V., Battista J. R.. ( 1996;). Radioresistance of Deinococcus radiodurans: functions necessary to survive ionizing radiation are also necessary to survive prolonged desiccation. . J Bacteriol 178:, 633–637.[PubMed]
    [Google Scholar]
  16. Osman S., Satomi M., Venkateswaran K.. ( 2006;). Paenibacillus pasadenensis sp. nov. and Paenibacillus barengoltzii sp. nov., isolated from a spacecraft assembly facility. . Int J Syst Evol Microbiol 56:, 1509–1514. [CrossRef][PubMed]
    [Google Scholar]
  17. Rainey F. A., Ray K., Ferreira M., Gatz B. Z., Nobre M. F., Bagaley D., Rash B. A., Park M. J., Earl A. M.. & other authors ( 2005;). Extensive diversity of ionizing-radiation-resistant bacteria recovered from Sonoran Desert soil and description of nine new species of the genus Deinococcus obtained from a single soil sample. . Appl Environ Microbiol 71:, 5225–5235. [CrossRef][PubMed]
    [Google Scholar]
  18. Satomi M., La Duc M. T., Venkateswaran K.. ( 2006;). Bacillus safensis sp. nov., isolated from spacecraft and assembly-facility surfaces. . Int J Syst Evol Microbiol 56:, 1735–1740. [CrossRef][PubMed]
    [Google Scholar]
  19. Schumann P.. ( 2011;). Peptidoglycan structure. In Taxonomy of Prokaryotes, Methods in Microbiology. , vol. 38, pp. 101. Edited by Rainey F., Oren A... London:: Elsevier;.
  20. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Stackebrandt E., Koch C., Gvozdiak O., Schumann P.. ( 1995;). Taxonomic dissection of the genus Micrococcus: Kocuria gen. nov., Nesterenkonia gen. nov., Kytococcus gen. nov., Dermacoccus gen. nov., and Micrococcus Cohn 1872 gen. emend. . Int J Syst Bacteriol 45:, 682–692. [CrossRef][PubMed]
    [Google Scholar]
  22. Suresh K., Reddy G. S., Sengupta S., Shivaji S.. ( 2004;). Deinococcus indicus sp. nov., an arsenic-resistant bacterium from an aquifer in West Bengal, India. . Int J Syst Evol Microbiol 54:, 457–461. [CrossRef][PubMed]
    [Google Scholar]
  23. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: Molecular Evolutionary Genetics Analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  24. Tindall B. J., Sikorski J., Smibert R. M., Krieg N. R.. ( 2007;). Phenotypic characterization and the principles of comparative systematics. In Methods for General and Molecular Microbiology. , pp. 332–393. Washington, DC:: American Society for Microbiology;.
  25. Vaishampayan P., Osman S., Andersen G., Venkateswaran K.. ( 2010;). High-density 16S microarray and clone library-based microbial community composition of the Phoenix spacecraft assembly clean room. . Astrobiology 10:, 499–508. [CrossRef][PubMed]
    [Google Scholar]
  26. Venkateswaran K., Kempf M., Chen F., Satomi M., Nicholson W., Kern R.. ( 2003;). Bacillus nealsonii sp. nov., isolated from a spacecraft-assembly facility, whose spores are gamma-radiation resistant. . Int J Syst Evol Microbiol 53:, 165–172. [CrossRef][PubMed]
    [Google Scholar]
  27. Yang Y., Itoh T., Yokobori S., Shimada H., Itahashi S., Satoh K., Ohba H., Narumi I., Yamagishi A.. ( 2010;). Deinococcus aetherius sp. nov., isolated from the stratosphere. . Int J Syst Evol Microbiol 60:, 776–779. [CrossRef][PubMed]
    [Google Scholar]
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