sp. nov., isolated from a typical sandy loam soil under long-term fertilization Free

Abstract

A Gram-staining-positive, endospore-forming, moderately alkaliphilic bacterium, strain NPK15, was isolated from a typical sandy loam soil under long-term NPK fertilization in northern China and was subjected to a polyphasic taxonomic study. The diamino acid of the cell-wall peptidoglycan of strain NPK15 was found to be -diaminopimelic acid and the cell-wall sugars were xylose, glucose and traces of mannose. The only respiratory quinone found in strain NPK15 was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C, anteiso-C, C and Cω6/Cω7. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis of the strain based on its 16S rRNA gene sequence showed that it was related most closely to ‘’ KACC 17216 (99.59 %), KCTC 13881 (99.52 %) and CCTCC AB 2012074 (99.00 %). DNA–DNA hybridization results indicated that the strain was distinct from other species of the genus , the degree of relatedness being 35.4 % with CCTCC AB 2012074, 39.7 % with KCTC 13881 and 51.2 % with ‘’ KACC 17216. The DNA G+C content of strain NPK15 was 45.5 mol%. Phenotypic, chemotaxonomic and molecular analyses identified strain NPK15 as a member of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NPK15 ( = DSM 26745 = CCTCC AB 2013156).

Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 41101229)
  • National Basic Research Program of China (Award 2011CB100505)
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2014-08-01
2024-03-28
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