1887

Abstract

A Gram-staining-positive, endospore-forming, moderately alkaliphilic bacterium, strain NPK15, was isolated from a typical sandy loam soil under long-term NPK fertilization in northern China and was subjected to a polyphasic taxonomic study. The diamino acid of the cell-wall peptidoglycan of strain NPK15 was found to be -diaminopimelic acid and the cell-wall sugars were xylose, glucose and traces of mannose. The only respiratory quinone found in strain NPK15 was menaquinone 7 (MK-7). The major cellular fatty acids were iso-C, anteiso-C, C and Cω6/Cω7. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. Phylogenetic analysis of the strain based on its 16S rRNA gene sequence showed that it was related most closely to ‘ ’ KACC 17216 (99.59 %), KCTC 13881 (99.52 %) and CCTCC AB 2012074 (99.00 %). DNA–DNA hybridization results indicated that the strain was distinct from other species of the genus , the degree of relatedness being 35.4 % with CCTCC AB 2012074, 39.7 % with KCTC 13881 and 51.2 % with ‘ ’ KACC 17216. The DNA G+C content of strain NPK15 was 45.5 mol%. Phenotypic, chemotaxonomic and molecular analyses identified strain NPK15 as a member of a novel species of the genus , for which the name sp. nov. is proposed. The type strain is NPK15 ( = DSM 26745 = CCTCC AB 2013156).

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2014-08-01
2020-01-19
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