sp. nov. and sp. nov., isolated from spoiled packaged Spanish-style green olives Free

Abstract

Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44 % with NCFB 563 and 98.33 and 98.11 % with M5, respectively, and a similarity of 99.41 % between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from NCFB 563 and M5. Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus . Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus , for which the names sp. nov. (type strain, IGBL1 = CECT 8061 = DSM 25436) and sp. nov. (type strain, IGBL13 = CECT 8062 = DSM 25450) are proposed.

Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (MICINN)
  • Spanish Ministry of Economy and Competitiveness (MINECO) (Award AGL2012-33400 and AGL2009-07861)
  • Junta de Andalucía Excellence Projects (Award AGR-07345 and AGR-04621)
  • FEDER
  • Spanish National Research Council (CSIC)
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2014-09-01
2024-03-28
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