Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44 % with Propionibacterium acidipropionici NCFB 563 and 98.33 and 98.11 % with Propionibacterium microaerophilum M5T, respectively, and a similarity of 99.41 % between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from P. acidipropionici NCFB 563 and P. microaerophilum M5T. Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus Propionibacterium. Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus Propionibacterium, for which the names Propionibacteriumolivae sp. nov. (type strain, IGBL1T = CECT 8061T = DSM 25436T) and Propionibacteriumdamnosum sp. nov. (type strain, IGBL13T = CECT 8062T = DSM 25450T) are proposed.
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Funding
This study was supported by the:
Spanish Ministry of Science and Innovation (MICINN)
Spanish Ministry of Economy and Competitiveness (MINECO)
(Award AGL2012-33400 and AGL2009-07861)
Junta de Andalucía Excellence Projects
(Award AGR-07345 and AGR-04621)
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