1887

Abstract

Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44 % with NCFB 563 and 98.33 and 98.11 % with M5, respectively, and a similarity of 99.41 % between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from NCFB 563 and M5. Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus . Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus , for which the names sp. nov. (type strain, IGBL1 = CECT 8061 = DSM 25436) and sp. nov. (type strain, IGBL13 = CECT 8062 = DSM 25450) are proposed.

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2014-09-01
2019-11-18
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References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J.. ( 1997;). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25:, 3389–3402. [CrossRef][PubMed]
    [Google Scholar]
  2. Butler-Wu S. M., Sengupta D. J., Kittichotirat W., Matsen F. A. III, Bumgarner R. E.. ( 2011;). Genome sequence of a novel species, Propionibacterium humerusii. . J Bacteriol 193:, 3678. [CrossRef][PubMed]
    [Google Scholar]
  3. Cathcart D. P.. ( 1995;). Purification, characterization and molecular analysis of plantaricin S, a two-peptide bacteriocin from olive fermenting Lactobacillus plantarum strains. Ph.D. Thesis. Cranfield University, Bedford, United Kingdom. .
    [Google Scholar]
  4. Cousin F. J., Mater D. D. G., Foligné B., Jan G.. ( 2011;). Dairy propionibacteria as human probiotics: a review of recent evidence. . Dairy Sci Technol 91:, 1–26. [CrossRef]
    [Google Scholar]
  5. Cummins C. S., Johnson J. L.. ( 1986;). Genus I. Propionibacterium. . In: Bergey’s Manual of Systematic Bacteriology, , 1st edn., pp. 1346–1353. Edited by Holt J. G... Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  6. de Castro A., Montaño A., Casado F.-J., Sánchez A.-H., Rejano L.. ( 2002;). Utilization of Enterococcus casseliflavus and Lactobacillus pentosus as starter culture for Spanish-style green olive fermentation. . Food Microbiol 19:, 637–644. [CrossRef]
    [Google Scholar]
  7. Garrido Fernández A., Fernández Díez M. J., Adams M. R.. ( 1997;). Green olive type. . In Table Olives: Production and Processing, pp. 134–206. Edited by Garrido Fernández A., Fernández Díez M. J., Adams M. R... London:: Chapman & Hall;. [CrossRef]
    [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C.. ( 2002;). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4:, 770–773. [CrossRef][PubMed]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C.. ( 2005;). A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles 9:, 75–79. [CrossRef][PubMed]
    [Google Scholar]
  10. González Cancho F., Fernández Díez M. J., Nosti Vega M., Buzcu N.. ( 1973;). Caracteristiques des principaux micro-organismes responsables de la “zapateria”. . Inf Oleicoles Int 60–61:, 133–144.
    [Google Scholar]
  11. González Cancho F., Rejano Navarro L., Borbolla y Alcalá J. M. R.. ( 1980;). La formacion de ácido propiónico durante la conservación de aceituna verdes de mesa. III. Micro-organismos responsables. . Grasas Aceites 31:, 245–250.
    [Google Scholar]
  12. Kawatomari T., Vaughn R. H.. ( 1956;). Species of Clostridium associated with zapatera spoilage of olives. . J Food Sci 21:, 481–490. [CrossRef]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  14. Koussémon M., Combet-Blanc Y., Patel B. K., Cayol J. L., Thomas P., Garcia J. L., Ollivier B.. ( 2001;). Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater. . Int J Syst Evol Microbiol 51:, 1373–1382.[PubMed]
    [Google Scholar]
  15. Kullen M. J., Sanozky-Dawes R. B., Crowell D. C., Klaenhammer T. R.. ( 2000;). Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex. . J Appl Microbiol 89:, 511–516. [CrossRef][PubMed]
    [Google Scholar]
  16. Kusano K., Yamada H., Niwa M., Yamasato K.. ( 1997;). Propionibacterium cyclohexanicum sp. nov., a new acid-tolerant ω-cyclohexyl fatty acid-containing propionibacterium isolated from spoiled orange juice. . Int J Syst Bacteriol 47:, 825–831. [CrossRef][PubMed]
    [Google Scholar]
  17. Lane D. J.. ( 1991;). 16/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E., Goodfellow M... Chichester:: Wiley;.
    [Google Scholar]
  18. Lee H. M., Lee Y.. ( 2008;). A differential medium for lactic acid-producing bacteria in a mixed culture. . Lett Appl Microbiol 46:, 676–681. [CrossRef][PubMed]
    [Google Scholar]
  19. Myers E. W., Miller W.. ( 1988;). Optimal alignments in linear space. . Comput Appl Biosci 4:, 11–17.[PubMed]
    [Google Scholar]
  20. Naylor J., Sharpe M. E.. ( 1958;). Lactobacilli in cheddar cheese: 1. The use of selective media for isolation and of serological typing for identification. . J Dairy Res 25:, 92–103. [CrossRef]
    [Google Scholar]
  21. Plastourgos S., Vaughn R. H.. ( 1957;). Species of Propionibacterium associated with zapatera spoilage of olives. . Appl Microbiol 5:, 267–271.[PubMed]
    [Google Scholar]
  22. Rejano L., Montaño A., Casado F. J., Sánchez A. H., de Castro A.. ( 2010;). Table olives: varieties and variations. . In Olives and Olive Oil in Health and Disease Prevention, pp. 5–15. Edited by Preedy V. R., Watson R. R... Oxford:: Academic Press;.
  23. Ruiz-Barba J. L., Jiménez-Díaz R.. ( 2012;). A novel Lactobacillus pentosus-paired starter culture for Spanish-style green olive fermentation. . Food Microbiol 30:, 253–259. [CrossRef][PubMed]
    [Google Scholar]
  24. Ruiz-Barba J. L., Maldonado A., Jiménez-Díaz R.. ( 2005;). Small-scale total DNA extraction from bacteria and yeast for PCR applications. . Anal Biochem 347:, 333–335. [CrossRef][PubMed]
    [Google Scholar]
  25. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  26. Sánchez A. H., Rejano L., Montaño A.. ( 1991;). Kinetics of the destruction by heat of colour and texture of pickled green olives. . J Sci Food Agric 54:, 379–385. [CrossRef]
    [Google Scholar]
  27. Sánchez Gomez A. H., García P. G., Rejano Navarro L.. ( 2006;). Elaboration of table olives. . Grasas Aceites 57:, 86–94. [CrossRef]
    [Google Scholar]
  28. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  29. Stackebrandt E., Cummins C. S., Johnson J. L.. ( 2006;). Family Propionibacteriaceae: the genus Propionibacterium. . In The Prokaryotes: A Handbook on the Biology of Bacteria, pp. 400–418. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E... New York:: Springer;. [CrossRef]
    [Google Scholar]
  30. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  31. Thompson J. D., Higgins D. G., Gibson T. J.. ( 1994;). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22:, 4673–4680. [CrossRef][PubMed]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
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