1887

Abstract

Five strains of Gram-stain-positive bacteria were isolated from anomalous fermentations occurring in post-packaging of sealed airtight food-grade plastic pouches of Spanish-style green olives. These isolates could be grouped into two sets, which showed a similarity in their respective 16S rRNA gene sequences of 98.40 and 98.44 % with NCFB 563 and 98.33 and 98.11 % with M5, respectively, and a similarity of 99.41 % between them. The 16S rRNA gene phylogeny revealed that the isolates grouped into two statistically well-supported clusters separate from NCFB 563 and M5. Enzymic activity profiles as well as fermentation patterns differentiated these two novel bacteria from other members of the genus . Finally, phenotypic, genotypic and phylogenetic data, supported the proposal of two novel species of the genus , for which the names sp. nov. (type strain, IGBL1 = CECT 8061 = DSM 25436) and sp. nov. (type strain, IGBL13 = CECT 8062 = DSM 25450) are proposed.

Funding
This study was supported by the:
  • Spanish Ministry of Science and Innovation (MICINN)
  • Spanish Ministry of Economy and Competitiveness (MINECO) (Award AGL2012-33400 and AGL2009-07861)
  • Junta de Andalucía Excellence Projects (Award AGR-07345 and AGR-04621)
  • FEDER
  • Spanish National Research Council (CSIC)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.063032-0
2014-09-01
2021-10-22
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/9/2980.html?itemId=/content/journal/ijsem/10.1099/ijs.0.063032-0&mimeType=html&fmt=ahah

References

  1. Altschul S. F., Madden T. L., Schäffer A. A., Zhang J., Zhang Z., Miller W., Lipman D. J. ( 1997 ). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res 25, 33893402. [View Article] [PubMed]
    [Google Scholar]
  2. Butler-Wu S. M., Sengupta D. J., Kittichotirat W., Matsen F. A. III, Bumgarner R. E. ( 2011 ). Genome sequence of a novel species, Propionibacterium humerusii . . J Bacteriol 193, 3678. [View Article] [PubMed]
    [Google Scholar]
  3. Cathcart D. P. ( 1995 ). Purification, characterization and molecular analysis of plantaricin S, a two-peptide bacteriocin from olive fermenting Lactobacillus plantarum strains. Ph.D. Thesis. Cranfield University, Bedford, United Kingdom. .
    [Google Scholar]
  4. Cousin F. J., Mater D. D. G., Foligné B., Jan G. ( 2011 ). Dairy propionibacteria as human probiotics: a review of recent evidence. . Dairy Sci Technol 91, 126. [View Article]
    [Google Scholar]
  5. Cummins C. S., Johnson J. L. ( 1986 ). Genus I. Propionibacterium . . In: Bergey’s Manual of Systematic Bacteriology, , 1st edn., pp. 13461353. Edited by Holt J. G. . Baltimore:: Williams & Wilkins;.
    [Google Scholar]
  6. de Castro A., Montaño A., Casado F.-J., Sánchez A.-H., Rejano L. ( 2002 ). Utilization of Enterococcus casseliflavus and Lactobacillus pentosus as starter culture for Spanish-style green olive fermentation. . Food Microbiol 19, 637644. [View Article]
    [Google Scholar]
  7. Garrido Fernández A., Fernández Díez M. J., Adams M. R. ( 1997 ). Green olive type. . In Table Olives: Production and Processing, pp. 134206. Edited by Garrido Fernández A., Fernández Díez M. J., Adams M. R. . London:: Chapman & Hall;. [View Article]
    [Google Scholar]
  8. Gonzalez J. M., Saiz-Jimenez C. ( 2002 ). A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. . Environ Microbiol 4, 770773. [View Article] [PubMed]
    [Google Scholar]
  9. Gonzalez J. M., Saiz-Jimenez C. ( 2005 ). A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles 9, 7579. [View Article] [PubMed]
    [Google Scholar]
  10. González Cancho F., Fernández Díez M. J., Nosti Vega M., Buzcu N. ( 1973 ). Caracteristiques des principaux micro-organismes responsables de la “zapateria”. . Inf Oleicoles Int 60–61, 133144.
    [Google Scholar]
  11. González Cancho F., Rejano Navarro L., Borbolla y Alcalá J. M. R. ( 1980 ). La formacion de ácido propiónico durante la conservación de aceituna verdes de mesa. III. Micro-organismos responsables. . Grasas Aceites 31, 245250.
    [Google Scholar]
  12. Kawatomari T., Vaughn R. H. ( 1956 ). Species of Clostridium associated with zapatera spoilage of olives. . J Food Sci 21, 481490. [View Article]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  14. Koussémon M., Combet-Blanc Y., Patel B. K., Cayol J. L., Thomas P., Garcia J. L., Ollivier B. ( 2001 ). Propionibacterium microaerophilum sp. nov., a microaerophilic bacterium isolated from olive mill wastewater. . Int J Syst Evol Microbiol 51, 13731382.[PubMed]
    [Google Scholar]
  15. Kullen M. J., Sanozky-Dawes R. B., Crowell D. C., Klaenhammer T. R. ( 2000 ). Use of the DNA sequence of variable regions of the 16S rRNA gene for rapid and accurate identification of bacteria in the Lactobacillus acidophilus complex. . J Appl Microbiol 89, 511516. [View Article] [PubMed]
    [Google Scholar]
  16. Kusano K., Yamada H., Niwa M., Yamasato K. ( 1997 ). Propionibacterium cyclohexanicum sp. nov., a new acid-tolerant ω-cyclohexyl fatty acid-containing propionibacterium isolated from spoiled orange juice. . Int J Syst Bacteriol 47, 825831. [View Article] [PubMed]
    [Google Scholar]
  17. Lane D. J. ( 1991 ). 16/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115175. Edited by Stackebrandt E., Goodfellow M. . Chichester:: Wiley;.
    [Google Scholar]
  18. Lee H. M., Lee Y. ( 2008 ). A differential medium for lactic acid-producing bacteria in a mixed culture. . Lett Appl Microbiol 46, 676681. [View Article] [PubMed]
    [Google Scholar]
  19. Myers E. W., Miller W. ( 1988 ). Optimal alignments in linear space. . Comput Appl Biosci 4, 1117.[PubMed]
    [Google Scholar]
  20. Naylor J., Sharpe M. E. ( 1958 ). Lactobacilli in cheddar cheese: 1. The use of selective media for isolation and of serological typing for identification. . J Dairy Res 25, 92103. [View Article]
    [Google Scholar]
  21. Plastourgos S., Vaughn R. H. ( 1957 ). Species of Propionibacterium associated with zapatera spoilage of olives. . Appl Microbiol 5, 267271.[PubMed]
    [Google Scholar]
  22. Rejano L., Montaño A., Casado F. J., Sánchez A. H., de Castro A. ( 2010 ). Table olives: varieties and variations. . In Olives and Olive Oil in Health and Disease Prevention , pp. 515. Edited by Preedy V. R., Watson R. R. . Oxford:: Academic Press;.
    [Google Scholar]
  23. Ruiz-Barba J. L., Jiménez-Díaz R. ( 2012 ). A novel Lactobacillus pentosus-paired starter culture for Spanish-style green olive fermentation. . Food Microbiol 30, 253259. [View Article] [PubMed]
    [Google Scholar]
  24. Ruiz-Barba J. L., Maldonado A., Jiménez-Díaz R. ( 2005 ). Small-scale total DNA extraction from bacteria and yeast for PCR applications. . Anal Biochem 347, 333335. [View Article] [PubMed]
    [Google Scholar]
  25. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  26. Sánchez A. H., Rejano L., Montaño A. ( 1991 ). Kinetics of the destruction by heat of colour and texture of pickled green olives. . J Sci Food Agric 54, 379385. [View Article]
    [Google Scholar]
  27. Sánchez Gomez A. H., García P. G., Rejano Navarro L. ( 2006 ). Elaboration of table olives. . Grasas Aceites 57, 8694. [View Article]
    [Google Scholar]
  28. Stackebrandt E., Goebel B. M. ( 1994 ). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44, 846849. [View Article]
    [Google Scholar]
  29. Stackebrandt E., Cummins C. S., Johnson J. L. ( 2006 ). Family Propionibacteriaceae: the genus Propionibacterium . . In The Prokaryotes: A Handbook on the Biology of Bacteria, pp. 400418. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  30. Tamura K., Dudley J., Nei M., Kumar S. ( 2007 ). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24, 15961599. [View Article] [PubMed]
    [Google Scholar]
  31. Thompson J. D., Higgins D. G., Gibson T. J. ( 1994 ). clustal w: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. . Nucleic Acids Res 22, 46734680. [View Article] [PubMed]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.063032-0
Loading
/content/journal/ijsem/10.1099/ijs.0.063032-0
Loading

Data & Media loading...

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error