1887

Abstract

Two actinomycete strains, KK1-2 and CPB4-7, were isolated from marine sediments collected in Chumphon province, Thailand. Chumphon province, Thailand. Their taxonomic positions were determined using a polyphasic approach. The morphological, cultural and chemotaxonomic characteristics of these isolates were consistent with the classification of the strains as representing a member of the genus . They contained -diaminopimelic acid in their cell wall peptidoglycan; the whole-cell sugars were ribose and glucose. The predominant menaquinones were MK9-(H) and MK9-(H). The major polar lipids were phosphatidylethanolamine, phosphatidylinositol, diphosphatidylglycerol, phosphatidylglycerol and phosphatidylinositol mannosides. The predominant cellular fatty acids were anteiso-C, iso-C and iso-C. On the basis of 16S rRNA gene sequence similarity studies, these isolates were determined to be closely related to JCM 16958 (98.2 %), JCM 4541 (98.1 %), JCM 4828 (98.1 %) and JCM 4476 (98.0 %). The G+C contents of the genomic DNA of strains KK1-2 and CPB4-7 were 73.3 and 74.2 mol%, respectively. They could be clearly distinguished from the related type strains by a low DNA–DNA relatedness and phenotypic differences. On the basis of these results, these strains represent a novel species of the genus , for which the name sp. nov. (type strain KK1-2 = JCM 18522 = TISTR 2106 = PCU 330) is proposed.

Funding
This study was supported by the:
  • , Thailand Research Fund
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2014-08-01
2020-08-04
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