1887

Abstract

Strain TS-56 was isolated from the gut of a wood-feeding termite, Snyder. Phylogenetic analyses based on 16S rRNA gene sequences revealed that the strain represented a member of the genus of the family , with sequence similarities to other species of the genus ranging from 96.6 % to 97.8 %. The isolate was Gram-stain-positive, non-motile, with light yellow colonies and irregular short rod-shaped cells (0.4–0.6 µm in diameter, 0.6–1.0 µm in length). Growth of TS-56 occurred at 20–35 °C (optimum, 30 °C) and at pH 4.0–8.0 (optimum, pH 5.0). The peptidoglycan of TS-56 contained ornithine, glutamic acid, alanine, homoserine and glycine. The acyl type was acetyl. The most abundant cellular fatty acid of TS-56 was cyclohexyl-C (88.79 %). The respiratory menaquinone was MK-11. The polar lipid profile contained disphosphatidylglycerol, phosphatidylglycerol, phosphatidylcholine, phosphatidylinositol and two unknown glycolipids. DNA of the type strain had a G+C content of 67.4 mol%. On the basis of the phylogenetic properties and phenotypic distinctiveness, TS-56 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is TS-56 ( = CGMCC 1.10363 = NBRC 109838).

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2015-01-01
2019-11-18
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References

  1. Altschul S. F., Gish W., Miller W., Myers E. W., Lipman D. J.. ( 1990;). Basic local alignment search tool. . J Mol Biol 215:, 403–410. [CrossRef][PubMed]
    [Google Scholar]
  2. Behrendt U., Ulrich A., Schumann P., Naumann D., Suzuki K.. ( 2002;). Diversity of grass-associated Microbacteriaceae isolated from the phyllosphere and litter layer after mulching the sward; polyphasic characterization of Subtercola pratensis sp. nov., Curtobacterium herbarum sp. nov. and Plantibacter flavus gen. nov., sp. nov.. Int J Syst Evol Microbiol 52:, 1441–1454. [CrossRef][PubMed]
    [Google Scholar]
  3. Chen W., Wang B., Hong H., Yang H., Liu S.-J.. ( 2012;). Deinococcus reticulitermitis sp. nov., isolated from a termite gut. . Int J Syst Evol Microbiol 62:, 78–83. [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D.. ( 1985;). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267–287. Edited by Goodfellow M., Minnikin D. E... London:: Academic Press;.
    [Google Scholar]
  5. Dong X.-Z., Cai M.-Y.. (editors) ( 2001;). Determination of biochemical properties. . In Manual for the Systematic Identification of General Bacteria, pp. 370–398. Beijing:: Science Press; (in Chinese).
    [Google Scholar]
  6. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  7. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  8. Kim M. K., Kim Y. J., Kim H. B., Kim S. Y., Yi T. H., Yang D. C.. ( 2008;). Curtobacterium ginsengisoli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 58:, 2393–2397. [CrossRef][PubMed]
    [Google Scholar]
  9. Kim H., Park D.-S., Oh H.-W., Lee K. H., Chung D.-H., Park H.-Y., Park H.-M., Bae K. S.. ( 2012a;). Gryllotalpicola gen. nov., with descriptions of Gryllotalpicola koreensis sp. nov., Gryllotalpicola daejeonensis sp. nov. and Gryllotalpicola kribbensis sp. nov. from the gut of the African mole cricket, Gryllotalpa africana, and reclassification of Curtobacterium ginsengisoli as Gryllotalpicola ginsengisoli comb. nov.. Int J Syst Evol Microbiol 62:, 2363–2370. [CrossRef][PubMed]
    [Google Scholar]
  10. Kim B.-C., Park D.-S., Kim H., Oh H.-W., Lee K. H., Shin K.-S., Bae K. S.. ( 2012b;). Herbiconiux moechotypicola sp. nov., a xylanolytic bacterium isolated from the gut of hairy long-horned toad beetles, Moechotypa diphysis (Pascoe). . Int J Syst Evol Microbiol 62:, 90–95. [CrossRef][PubMed]
    [Google Scholar]
  11. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  12. Park Y. H., Suzuki K., Yim D. G., Lee K. C., Kim E., Yoon J., Kim S., Kho Y. H., Goodfellow M., Komagata K.. ( 1993;). Suprageneric classification of peptidoglycan group B actinomycetes by nucleotide sequencing of 5S ribosomal RNA. . Antonie van Leeuwenhoek 64:, 307–313. [CrossRef][PubMed]
    [Google Scholar]
  13. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  14. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  15. Tamura K., Dudley J., Nei M., Kumar S.. ( 2007;). mega4: molecular evolutionary genetics analysis (mega) software version 4.0. . Mol Biol Evol 24:, 1596–1599. [CrossRef][PubMed]
    [Google Scholar]
  16. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G.. ( 1997;). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25:, 4876–4882. [CrossRef][PubMed]
    [Google Scholar]
  17. Uchida K., Kudo T., Suzuki K.-I., Nakase T.. ( 1999;). A new rapid method of glycolate test by diethyl ether extraction, which is applicable to a small amount of bacterial cells of less than one milligram. . J Gen Appl Microbiol 45:, 49–56. [CrossRef][PubMed]
    [Google Scholar]
  18. Wu C., Lu X., Qin M., Wang Y., Ruan J.. ( 1989;). Analysis of menaquinone compound in microbial cells by HPLC.. Microbiology [English translation of Microbiology (Beijing)] 16:, 176–178. [CrossRef]
    [Google Scholar]
  19. Yamada K., Komagata K.. ( 1972;). Taxonomic studies on coryneform bacteria. V. Classification of coryneform bacteria. . J Gen Appl Microbiol 18:, 417–431. [CrossRef]
    [Google Scholar]
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