1887

INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo

Volume 64, Issue Pt_8

sp. nov., a chloroacetamide herbicide–degrading bacterium isolated from activated sludge Free

Abstract

Strain BUT-14, a Gram-reaction-negative, non-spore-forming, ellipse-shaped bacterium, was isolated from activated sludge of a chloroacetamide-herbicides-manufacturing wastewater treatment facility. The strain was able to degrade more than 90 % of butachlor, acetochlor and alachlor (100 mg l) within 5 days of incubation. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BUT-14 was a member of the genus and showed the highest sequence similarities to DSM 22821 (97.9 %), KACC 15258 (97.4 %), JCM 12182 (97.4 %) DSM 25411 (97.1 %) and lower (<97 %) sequence similarities to all other species of the genus . Chemotaxonomic analysis revealed that strain BUT-14 possessed Q-10 as the predominant ubiquinone, spermidine as the major polyamine and Cω7 (46.9 %), Cω6 (17.9 %), summed feature 3, C 2-OH (4.4 %), C 2-OH (3.1 %) and C (5.51 %) as the major fatty acids. The polar lipids included lipid, glycolipid, phosphatidylglycerol, phospholipid, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and phospatidyldimethylethanolamine. Strain BUT-14 showed low DNA–DNA relatedness with DSM 22821 (41.5±2.9 %), JCM 12182 (49.2±4.2 %), KACC 12295 (53.2±1.9 %) DSM 25411 (51.2±4.5 %). The DNA G+C content was 66±0.3 mol%. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic data and DNA–DNA hybridization supports the suggestion that strain BUT-14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BUT-14 ( = CCTCC AB 2013086 = KACC 17147 = JCM 19923).

  • Published Online:
Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31270157)
  • National Science and Technology Support Plan (Award 2012BAD15B03 and 2012AA101403)
© 2014 IUMS
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062950-0
2014-08-01
2024-04-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2573.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062950-0&mimeType=html&fmt=ahah

References

  1. Arden Jones M. P., McCarthy A. J., Cross T. ( 1979 ). Taxonomic and serologic studies on Micropolyspora faeni and Micropolyspora strains from soil bearing the specific epithet rectivirgula . . J Gen Microbiol 115, 343–354. [View Article] [PubMed]
    [Google Scholar]
  2. Bernardet J. F., Nakagawa Y., Holmes B. Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002 ). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52, 1049–1070. [View Article] [PubMed]
    [Google Scholar]
  3. Buck J. D. ( 1982 ). Nonstaining (KOH) method for determination of Gram reactions of marine bacteria. . Appl Environ Microbiol 44, 992–993.[PubMed]
    [Google Scholar]
  4. Busse H.-J., Auling G. ( 1988 ). Polyamine pattern as a chemotaxonomic marker within the Proteobacteria . . Syst Appl Microbiol 11, 1–8. [View Article]
    [Google Scholar]
  5. Busse H.-J., Kämpfer P., Denner E. B. M. ( 1999 ). Chemotaxonomic characterisation of Sphingomonas . . J Ind Microbiol Biotechnol 23, 242–251. [View Article] [PubMed]
    [Google Scholar]
  6. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230. [View Article] [PubMed]
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224–229. [View Article]
    [Google Scholar]
  8. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  10. Kim N. H., Kim D. U., Kim I., Ka J.-O. ( 2013 ). Syntrophic biodegradation of butachlor by Mycobacterium sp. J7A and Sphingobium sp. J7B isolated from rice paddy soil. . FEMS Microbiol Lett 344, 114–120. [View Article] [PubMed]
    [Google Scholar]
  11. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  12. Lal R., Dogra C., Malhotra S., Sharma P., Pal R. ( 2006 ). Diversity, distribution and divergence of lin genes in hexachlorocyclohexane-degrading sphingomonads. . Trends Biotechnol 24, 121–130. [View Article] [PubMed]
    [Google Scholar]
  13. Lane D. L. ( 1991 ). 16S/23S rRNA sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by Stackebrandt E. R., Goodfellow M. . Chichester:: Wiley;.
     [Google Scholar]
  14. Lee K. B., Liu C. T., Anzai Y., Kim H., Aono T., Oyaizu H. ( 2005 ). The hierarchical system of the ‘Alphaproteobacteria’: description of Hyphomonadaceae fam. nov., Xanthobacteraceae fam. nov. and Erythrobacteraceae fam. nov.. Int J Syst Evol Microbiol 55, 1907–1919. [View Article] [PubMed]
    [Google Scholar]
  15. Li Y., Chen Q., Wang C. H., Cai S., He J., Huang X., Li S. P. ( 2013 ). Degradation of acetochlor by consortium of two bacterial strains and cloning of a novel amidase gene involved in acetochlor-degrading pathway. . Bioresour Technol 148, 628–631. [View Article] [PubMed]
    [Google Scholar]
  16. Liu Z. P., Wang B. J., Liu Y. H., Liu S. J. ( 2005 ). Novosphingobium taihuense sp. nov., a novel aromatic-compound-degrading bacterium isolated from Taihu Lake, China. . Int J Syst Evol Microbiol 55, 1229–1232. [View Article] [PubMed]
    [Google Scholar]
  17. Mandel M., Marmur J. ( 1968 ). Use of ultraviolet absorbance-temperature profile for determining the guanine plus cytosine content of DNA. . Methods Enzymol 12B, 195–206. [View Article]
    [Google Scholar]
  18. McCarthy A. J., Cross T. ( 1984 ). A taxonomic study of Thermomonospora and other monosporic Actinomycetes . . J Gen Microbiol 130, 5–25.
     [Google Scholar]
  19. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  20. Sambrook J., Russell D. W. ( 2001 ). Molecular Cloning: a Laboratory Manual, , 3rd edn.. Cold Spring Harbor, NY:: Cold Spring Harbor Laboratory;.
     [Google Scholar]
  21. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids. MIDI Technical Note 101. Newark, DE:: MIDI;.
     [Google Scholar]
  22. Sohn J. H., Kwon K. K., Kang J. H., Jung H. B., Kim S. J. ( 2004 ). Novosphingobium pentaromativorans sp. nov., a high-molecular-mass polycyclic aromatic hydrocarbon-degrading bacterium isolated from estuarine sediment. . Int J Syst Evol Microbiol 54, 1483–1487. [View Article] [PubMed]
    [Google Scholar]
  23. Stamper D. M., Tuovinen O. H. ( 1998 ). Biodegradation of the acetanilide herbicides alachlor, metolachlor, and propachlor. . Crit Rev Microbiol 24, 1–22. [View Article] [PubMed]
    [Google Scholar]
  24. Su G. X., Zhang W. H., Liu Y. L. ( 2006 ). Involvement of hydrogen peroxide generated by polyamine oxidative degradation in the development of lateral roots in soybean. . J Integr Plant Biol 48, 426–432. [View Article]
    [Google Scholar]
  25. Takeuchi M., Hamana K., Hiraishi A. ( 2001 ). Proposal of the genus Sphingomonas sensu stricto and three new genera, Sphingobium, Novosphingobium and Sphingopyxis, on the basis of phylogenetic and chemotaxonomic analyses. . Int J Syst Evol Microbiol 51, 1405–1417.[PubMed]
    [Google Scholar]
  26. Tamaoka J., Katayama-Fujimura Y., Kuraishi H. ( 1983 ). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54, 31–36. [View Article]
    [Google Scholar]
  27. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  28. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 4876–4882. [View Article] [PubMed]
    [Google Scholar]
  29. Tiirola M. A., Busse H. J., Kämpfer P., Männistö M. K. ( 2005 ). Novosphingobium lentum sp. nov., a psychrotolerant bacterium from a polychlorophenol bioremediation process. . Int J Syst Evol Microbiol 55, 583–588. [View Article] [PubMed]
    [Google Scholar]
  30. Tindall B. J. ( 1990a ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128–130. [View Article]
    [Google Scholar]
  31. Tindall B. J. ( 1990b ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199–202. [View Article]
    [Google Scholar]
  32. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  33. Zhang J., Zheng J. W., Liang B., Wang C. H., Cai S., Ni Y. Y., He J., Li S. P. ( 2011 ). Biodegradation of chloroacetamide herbicides by Paracoccus sp. FLY-8 in vitro . . J Agric Food Chem 59, 4614–4621. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062950-0
Loading
Novosphingobiumchloroacetimidivorans sp. nov., a chloroacetamide herbicide–degrading bacterium isolated from activated sludge
Int J Syst Evol Microbiol 64, 2573 (2014); https://doi.org/10.1099/ijs.0.062950-0
/content/journal/ijsem/10.1099/ijs.0.062950-0
/content/journal/ijsem/10.1099/ijs.0.062950-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error