1887

Abstract

Strain BUT-14, a Gram-reaction-negative, non-spore-forming, ellipse-shaped bacterium, was isolated from activated sludge of a chloroacetamide-herbicides-manufacturing wastewater treatment facility. The strain was able to degrade more than 90 % of butachlor, acetochlor and alachlor (100 mg l) within 5 days of incubation. The taxonomic position was investigated using a polyphasic approach. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain BUT-14 was a member of the genus and showed the highest sequence similarities to DSM 22821 (97.9 %), KACC 15258 (97.4 %), JCM 12182 (97.4 %) DSM 25411 (97.1 %) and lower (<97 %) sequence similarities to all other species of the genus . Chemotaxonomic analysis revealed that strain BUT-14 possessed Q-10 as the predominant ubiquinone, spermidine as the major polyamine and Cω7 (46.9 %), Cω6 (17.9 %), summed feature 3, C 2-OH (4.4 %), C 2-OH (3.1 %) and C (5.51 %) as the major fatty acids. The polar lipids included lipid, glycolipid, phosphatidylglycerol, phospholipid, phosphatidylethanolamine, phosphatidylcholine, sphingoglycolipid and phospatidyldimethylethanolamine. Strain BUT-14 showed low DNA–DNA relatedness with DSM 22821 (41.5±2.9 %), JCM 12182 (49.2±4.2 %), KACC 12295 (53.2±1.9 %) DSM 25411 (51.2±4.5 %). The DNA G+C content was 66±0.3 mol%. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic data and DNA–DNA hybridization supports the suggestion that strain BUT-14 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is BUT-14 ( = CCTCC AB 2013086 = KACC 17147 = JCM 19923).

Funding
This study was supported by the:
  • , National Natural Science Foundation of China , (Award 31270157)
  • , National Science and Technology Support Plan , (Award 2012BAD15B03 and 2012AA101403)
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2014-08-01
2020-07-10
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