1887

Abstract

A novel Gram-stain-negative, aerobic, non-endospore-forming, non-pigmented, rod-shaped, slightly halophilic bacterium, designated GBPy5, was isolated from aquatic plants of the Gomishan wetland, Iran. Cells of strain GBPy5 were motile. Growth occurred with between 1 and 10 % (w/v) NaCl and the isolate grew optimally with 3 % (w/v) NaCl. The optimum pH and temperature for growth of the strain were pH 8.0 and 30 °C, respectively, while it was able to grow over a pH range of 6.5–9.0 and a temperature range of 4–35 °C. Phylogenetic analysis, based on 16S rRNA gene sequences, revealed that strain GBPy5 is a member of the genus forming a monophyletic branch. The novel strain exhibited 16S rRNA gene sequence similarity of 95.4 % with type strains of PCAVU11 and J64, respectively. The major cellular fatty acids of the isolate were Cω7 (37.8 %), C (14.9 %), Cω7 (12.9 %), C 3-OH (7.1 %) and C (7.0 %). The polar lipid pattern of strain GBPy5 comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine and one phospholipid. Ubiquinone 9 (Q-9) was the predominant lipoquinone. The G+C content of the genomic DNA of strain GBPy5 was 59.2 mol%. On the basis of the phenotypic and phylogenetic data, strain GBPY5 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is GBPy5 ( = IBRC-M 10762 = CECT 8338).

Funding
This study was supported by the:
  • , Iranian Biological Resource Centre , (Award MI-1391-15)
  • , Spanish Ministerio de Economía y Competitividad , (Award CGL2013-46941-P and CGL2010-19303)
  • , European Funds
  • , Junta de Andalucía , (Award P10-CVI-6226)
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2014-10-01
2021-01-27
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References

  1. Anzai Y., Kim H., Park J. Y., Wakabayashi H., Oyaizu H. ( 2000 ). Phylogenetic affiliation of the pseudomonads based on 16S rRNA sequence. . Int J Syst Evol Microbiol 50, 15631589. [CrossRef] [PubMed]
    [Google Scholar]
  2. Baron E. J., Finegold S. M. ( 1990 ). Bailey and Scott’s Diagnostic Microbiology, , 8th edn.. St Louis, MO:: Mosby;.
    [Google Scholar]
  3. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461466. [CrossRef] [PubMed]
    [Google Scholar]
  4. De Ley J. ( 1992 ). The proteobacteria: ribosomal RNA cistron similarities and bacterial taxonomy. . In The Prokaryotes, , 2nd edn., pp. 21112140. Edited by Balows A., Trüper H. G., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;.
    [Google Scholar]
  5. Euzéby J. P. ( 1997 ). List of bacterial names with standing in nomenclature: a folder available on the Internet. . Int J Syst Bacteriol 47, 590592. http://www.bacterio.net [CrossRef] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using bootstrap. . Evololution 39, 783791. [CrossRef]
    [Google Scholar]
  8. Fendrich C. ( 1988 ). Halovibrio variabilis gen. nov. sp. nov., Pseudomonas halophila sp. nov. and a new halophilic aerobic coccoid eubacterium from Great Salt Lake, Utah, USA. . Syst Appl Microbiol 11, 3643. [CrossRef]
    [Google Scholar]
  9. Groth I., Schumann P., Weiss N., Martin K., Rainey F. A. ( 1996 ). Agrococcus jenensis gen. nov., sp. nov., a new genus of actinomycetes with diaminobutyric acid in the cell wall. . Int J Syst Bacteriol 46, 234239. [CrossRef] [PubMed]
    [Google Scholar]
  10. Harrigan W. F., McCance M. E. ( 1976 ). Laboratory Methods in Food and Dairy Microbiology. London:: Academic Press;.
    [Google Scholar]
  11. Humm H. J. ( 1946 ). Marine agar-digesting bacteria of the South Atlantic coast. . Bull Duke Univ Mar Sta 3, 4575.
    [Google Scholar]
  12. Kämpfer P., Kroppenstedt R. M. ( 1996 ). Numerical analysis of fatty acid patterns of coryneform bacteria and related taxa. . Can J Microbiol 42, 9891005. [CrossRef]
    [Google Scholar]
  13. Kim K. H., Roh S. W., Chang H. W., Nam Y. D., Yoon J. H., Jeon C. O., Oh H. M., Bae J. W. ( 2009 ). Pseudomonas sabulinigri sp. nov., isolated from black beach sand. . Int J Syst Evol Microbiol 59, 3841. [CrossRef] [PubMed]
    [Google Scholar]
  14. King E. O., Ward M. K., Raney D. E. ( 1954 ). Two simple media for the demonstration of pyocyanin and fluorescin. . J Lab Clin Med 44, 301307.[PubMed]
    [Google Scholar]
  15. Kushner D. J., Kamekura M. ( 1988 ). Physiology of halophilic eubacteria. . In Halophilic Bacteria, vol. I, pp. 109140. Edited by Rodriguez-Valera F. . Boca Raton, FL:: CRC Press;.
    [Google Scholar]
  16. Lane D. J., Pace B., Olsen G. J., Stahl D. A., Sogin M. L., Pace N. R. ( 1985 ). Rapid determination of 16S ribosomal RNA sequences for phylogenetic analyses. . Proc Natl Acad Sci U S A 82, 69556959. [CrossRef] [PubMed]
    [Google Scholar]
  17. Liu M., Luo X., Zhang L., Dai J., Wang Y., Tang Y., Li J., Sun T., Fang C. ( 2009 ). Pseudomonas xinjiangensis sp. nov., a moderately thermotolerant bacterium isolated from desert sand. . Int J Syst Evol Microbiol 59, 12861289. [CrossRef] [PubMed]
    [Google Scholar]
  18. Ludwig W., Strunk O., Westram R., Richter L., Meier H., Yadhukumar, Buchner A., Lai T., Steppi S. et al. ( 2004 ). arb: a software environment for sequence data. . Nucleic Acids Res 32, 13631371. [CrossRef] [PubMed]
    [Google Scholar]
  19. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. ( 2002 ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25, 360375. [CrossRef] [PubMed]
    [Google Scholar]
  20. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Evol Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  21. Migula W. ( 1894 ). Über ein neues system der bakterien. . Arb Bakteriol Inst Technisch Hochsch Karlsruhe 1, 235238 (in German).
    [Google Scholar]
  22. Murray R. G. E., Doetsch R. N., Robinow C. F. ( 1994 ). Determinative and cytological light microscopy. . In Methods for General and Molecular Bacteriology, pp. 2141. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  23. Palleroni N. J. ( 1992 ). Introduction to the family Pseudomonadaceae . . In The Prokaryotes, , 2nd edn., pp. 30713085. Edited by Balows A., Truper H. G., Umlaut U., Dworkin M., Harder W., Schleifer K. H. . New York:: Springer;.
    [Google Scholar]
  24. Palleroni N. J. ( 2005 ). Genus I. Pseudomonas Migula 1894, 237AL . . In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, part B, pp. 323379. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;.
    [Google Scholar]
  25. Palleroni N. J., Doudoroff M. ( 1972 ). Some properties and subdivisions of the genus Pseudomonas . . Annu Rev Phytopathol 10, 73100. [CrossRef]
    [Google Scholar]
  26. Quesada E., Ventosa A., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1984 ). Deleya halophila, a new species of moderately halophilic bacteria. . Int J Syst Bacteriol 34, 287292. [CrossRef]
    [Google Scholar]
  27. Romanenko L. A., Uchino M., Falsen E., Frolova G. M., Zhukova N. V., Mikhailov V. V. ( 2005 ). Pseudomonas pachastrellae sp. nov., isolated from a marine sponge. . Int J Syst Evol Microbiol 55, 919924. [CrossRef] [PubMed]
    [Google Scholar]
  28. Rzhetsky A., Nei M. ( 1992 ). A simple method for estimating and testing minimum-evolution trees. . Mol Biol Evol 9, 945967.
    [Google Scholar]
  29. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  30. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  31. Stanier R. Y., Palleroni N. J., Doudoroff M. ( 1966 ). The aerobic pseudomonads: a taxonomic study. . J Gen Microbiol 43, 159271. [CrossRef] [PubMed]
    [Google Scholar]
  32. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  33. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  34. Toro M., Ramírez-Bahena M. H., Cuesta M. J., Velázquez E., Peix A. ( 2013 ). Pseudomonas guariconensis sp. nov., isolated from rhizospheric soil. . Int J Syst Evol Microbiol 63, 44134420. [CrossRef] [PubMed]
    [Google Scholar]
  35. Ventosa A., Quesada E., Rodríguez-Valera F., Ruiz-Berraquero F., Ramos-Cormenzana A. ( 1982 ). Numerical taxonomy of moderately halophilic Gram-negative rods. . J Gen Microbiol 128, 19591968.
    [Google Scholar]
  36. Yoon J. H., Kim H., Kang K. H., Oh T. K., Park Y. H. ( 2003 ). Transfer of Pseudomonas elongata Humm 1946 to the genus Microbulbifer as Microbulbifer elongatus comb. nov.. Int J Syst Evol Microbiol 53, 13571361. [CrossRef] [PubMed]
    [Google Scholar]
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