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Volume 65, Issue Pt_1

gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the ‘deep sea-1’ clade of marine methanotrophs Free

Abstract

We report the isolation and growth characteristics of a gammaproteobacterial methane-oxidizing bacterium ( strain WF1, ‘whale fall 1’) that shares 98 % 16S rRNA gene sequence identity with uncultivated free-living methanotrophs and the methanotrophic endosymbionts of deep-sea mussels, ≤94.6 % 16S rRNA gene sequence identity with species of the genus and ≤93.6 % 16S rRNA gene sequence identity with species of the genera and . Strain WF1 represents the first cultivar from the ‘deep sea-1’ clade of marine methanotrophs, which includes members that participate in methane oxidation in sediments and the water column in addition to mussel endosymbionts. Cells of strain WF1 were elongated cocci, approximately 1.5 µm in diameter, and occurred singly, in pairs and in clumps. The cell wall was Gram-negative, and stacked intracytoplasmic membranes and storage granules were evident. The genomic DNA G+C content of WF1 was 40.5 mol%, significantly lower than that of currently described cultivars, and the major fatty acids were 16 : 0, 16 : 1ω9, 16 : 1ω9, 16 : 1ω8 and 16 : 2ω9,14. Growth occurred in liquid media at an optimal temperature of 23 °C, and was dependent on the presence of methane or methanol. Atmospheric nitrogen could serve as the sole nitrogen source for WF1, a capacity that had not been functionally demonstrated previously in members of . On the basis of its unique morphological, physiological and phylogenetic properties, this strain represents the type species within a new genus, and we propose the name gen. nov., sp. nov. The type strain of is WF1 ( = LMG 28393 = ATCC BAA-2619).

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Funding
This study was supported by the:
  • NASA ASTEP (Award J 3162-B20 and NNG06GB34G)
  • National Science Foundation OCE (Award MCB-0348492)
  • Erwin Schrodinger Postdoctoral Fellowship of the Austrian Science Fund (FWF)
© 2015 IUMS
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2015-01-01
2024-04-19
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Methyloprofundus sedimenti gen. nov., sp. nov., an obligate methanotroph from ocean sediment belonging to the ‘deep sea-1’ clade of marine methanotrophs
Int J Syst Evol Microbiol 65, 251 (2015); https://doi.org/10.1099/ijs.0.062927-0
/content/journal/ijsem/10.1099/ijs.0.062927-0
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