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Abstract

A bacterial strain, designated RHs22, was isolated from a soil sample cultivated with rice in the Suwon region of South Korea. The cells were aerobic, Gram-stain-negative, non-spore-forming, non-flagellated rods or occasionally filaments. The strain grew at 10–37 °C (optimum, 28–30 °C), at pH 5.0–10.0 (optimum, 7.0) and in the presence of 0–1 % (w/v) NaCl (optimum, 0 %). Phylogenetically, the strain was closely related to members of the genus , as its 16S rRNA gene sequence had similarity of 90.3–92.1 % with respect to those of members of the genus , showing the highest sequence similarity with DSM 21099. Strain RHs22 revealed relatively low sequence similarities of less than 90 % with all the other species with validly published names. It contained MK-7 as the predominant menaquinone and summed feature 3 (Cω6 and/or Cω7), Cω5, iso-C and iso-C 3-OH as the main fatty acids. The polar lipids of strain RHs22 were phosphatidylethanolamine, one unknown aminolipid, two unknown aminophospholipids, one unknown phospholipid and five unknown lipids. The DNA G+C content was 57.0 mol%. Phylogenetic, phenotypic and chemotaxonomic data obtained in this study indicate that strain RHs22 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is RHs22 ( = KACC 17324 = DSM 28354). An emended description of the genus is also proposed.

Funding
This study was supported by the:
  • , National Academy of Agricultural Science, Rural Development Administration, Republic of Korea , (Award Project no. PJ008666)
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2014-09-01
2021-03-08
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