Two catalase- and oxidase-negative Streptococcus-like strains, LMG 27682T and LMG 27684T, were isolated from raw camel milk in Morocco. Comparative 16S rRNA gene sequencing assigned these bacteria to the genus Streptococcus with Streptococcus rupicaprae 2777-2-07T as their closest phylogenetic neighbour (95.9 % and 95.7 % similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.7 %. Although strains LMG 27682T and LMG 27684T shared a DNA–DNA hybridization value that corresponded to the threshold level for species delineation (68 %), the two strains could be distinguished by multiple biochemical tests, sequence analysis of the phenylalanyl-tRNA synthase (pheS), RNA polymerase (rpoA) and ATP synthase (atpA) genes and by their MALDI-TOF MS profiles. On the basis of these considerable phenotypic and genotypic differences, we propose to classify both strains as novel species of the genus Streptococcus, for which the names Streptococcusmoroccensis sp. nov. (type strain, LMG 27682T = CCMM B831T) and Streptococcusrifensis sp. nov. (type strain, LMG 27684T = CCMM B833T) are proposed.
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Funding
This study was supported by the:
Belgian General Administration for International Cooperation
(Award FRAB/2005)
Centre National pour la Recherche Scientifique et Technique, Rabat, Morocco
(Award Programme d’urgence 2009–2012)
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