sp. nov. and sp. nov., isolated from raw camel milk Free

Abstract

Two catalase- and oxidase-negative -like strains, LMG 27682 and LMG 27684, were isolated from raw camel milk in Morocco. Comparative 16S rRNA gene sequencing assigned these bacteria to the genus with 2777-2-07 as their closest phylogenetic neighbour (95.9 % and 95.7 % similarity, respectively). 16S rRNA gene sequence similarity between the two strains was 96.7 %. Although strains LMG 27682 and LMG 27684 shared a DNA–DNA hybridization value that corresponded to the threshold level for species delineation (68 %), the two strains could be distinguished by multiple biochemical tests, sequence analysis of the phenylalanyl-tRNA synthase (), RNA polymerase () and ATP synthase () genes and by their MALDI-TOF MS profiles. On the basis of these considerable phenotypic and genotypic differences, we propose to classify both strains as novel species of the genus , for which the names sp. nov. (type strain, LMG 27682 = CCMM B831) and sp. nov. (type strain, LMG 27684 = CCMM B833) are proposed.

Funding
This study was supported by the:
  • Belgian General Administration for International Cooperation (Award FRAB/2005)
  • Centre National pour la Recherche Scientifique et Technique, Rabat, Morocco (Award Programme d’urgence 2009–2012)
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2014-07-01
2024-03-29
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