1887

Abstract

The terrestrial oxytrichid ciliate n. sp., isolated from soil samples collected from the uplands of Colfiorito (Umbria region, Italy), was investigated using live observation and protargol impregnation. The morphology, morphogenesis and molecular phylogeny inferred from small-subunit (SSU) rRNA gene sequences were studied. The novel species is mainly characterized by the following: a cell size of about 145×35 µm ; two ellipsoidal macronuclear nodules and two to four micronuclei; adoral zone about 26 % of body length with a mean of 30 membranelles; about 40 cirri in the right marginal row and 38 in the left marginal row; left fronto-ventral row consisting of about 27–40 cirri, right fronto-ventral row of about three to seven cirri forming a short row to the right of the rear portion of the left fronto-ventral row; one parabuccal cirrus ( = III/2), one buccal and one post-peristomial cirrus; and four dorsal kineties with caudal cirri at the end of kineties 1 and 2. The morphogenesis of the novel species is similar to that of . Phylogenetic analyses based on SSU rRNA gene sequences consistently placed the novel species within the family Oxytrichidae Ehrenberg, 1838, clustering with and the genus . The results from the present study contribute to the expanding knowledge of the diversity of ciliates in Italian soil.

Funding
This study was supported by the:
  • School of Environmental Sciences, University of Camerino, Italy
  • Italian Minister of University and Research (MIUR)
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2014-08-01
2024-03-28
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References

  1. Ammermann D., Steinbrück G., von Berger L., Hennig W. ( 1974 ). The development of the macronucleus in the ciliated protozoan Stylonychia mytilus . . Chromosoma 45, 401429. [View Article] [PubMed]
    [Google Scholar]
  2. Berger H. ( 1999). Monograph of the Oxytrichidae (Ciliophora, Hypotrichia) ( Monographiae Biologicae , vol. 78). Berlin:: Springer;. [View Article]
    [Google Scholar]
  3. Berger H. ( 2008). Monograph of the Amphisiellidae and Trachelostylidae (Ciliophora, Hypotricha) ( Monographiae Biologicae , vol. 88). Berlin:: Springer;.
    [Google Scholar]
  4. Chen X., Miao M., Ma H., Shao C., Al-Rasheid K. A. ( 2013 ). Morphology, morphogenesis and small-subunit rRNA gene sequence of the novel brackish-water ciliate Strongylidium orientale sp. nov. (Ciliophora, Hypotrichia). . Int J Syst Evol Microbiol 63, 11551164. [View Article] [PubMed]
    [Google Scholar]
  5. Ehrenberg C. G. ( 1838 ). Die Infusionsthierchen als vollkommene Organismen. Ein Blick in das tiefere organische Leben der Natur. Leipzig:: L. Voss (in German);. [View Article]
    [Google Scholar]
  6. Eigner P. ( 1997 ). Evolution of morphogenetic processes in the Orthoamphisiellidae n. fam., Oxytrichidae, and Parakahliellidae n. fam., and their depiction using a computer method (Ciliophora, Hypotrichida). . J Eukaryot Microbiol 44, 553573. [View Article]
    [Google Scholar]
  7. Foissner W. ( 1982 ). Ökologie und Taxonomie der Hypotrichida (Protozoa: Ciliophora) einiger österreichischer Böden. . Arch Protistenkd 126, 19143 (in German). [View Article]
    [Google Scholar]
  8. Foissner W. ( 1984 ). Infraciliatur, Silberliniensystem und Biometrie einiger neuer und wenig bekannter terrestrischer, limnischer und mariner Ciliaten (Protozoa: Ciliophora) aus den Klassen Kinetofragminophora, Colpodea und Polyhymenophora (Stapfia , vol. 12). Linz:: Oberösterreichisches Landesmuseum (in German);.
    [Google Scholar]
  9. Foissner W. ( 1987a ). Soil protozoa: fundamental problems, ecological significance, adaptations in ciliates and testaceans, bioindicators, and guide to the literature. . In Progress in Protistology, vol. 2, pp. 69212. Edited by Patterson D. J., Corliss J. O. . Bristol:: Biopress;.
    [Google Scholar]
  10. Foissner W. ( 1987b ). Neue und wenig bekannte hypotriche und colpodide Ciliaten (Protozoa: Ciliophora) aus Böden und Moosen. . Zool Beitr 31, 187282 (in German).
    [Google Scholar]
  11. Foissner W. ( 1988 ). In Gemeinsame Arten in der terricolen Ciliatenfauna (Protozoa: Ciliophora) von Australien und Afrika ( Stapfia , vol. 17), pp. 85133. Linz:: Oberösterreichisches Landesmuseum (in German);.
    [Google Scholar]
  12. Foissner W., Foissner I. (editors) ( 1988 ). Ciliophora ( Catalogus Faunae Austriae , vol. 1c). Wien:: Verlag der Österreichischen Akademie der Wissenschaften (in German);.
    [Google Scholar]
  13. Foissner W., Agatha S., Berger H. ( 2002 ). Soil ciliates (Protozoa, Ciliophora) from Namibia (Southwest Africa), with emphasis on two contrasting environments, the Etosha region and the Namib desert ( Denisia , vol. 5). Linz:: Oberösterreichisches Landesmuseum;.
    [Google Scholar]
  14. Glez-Peña D., Gómez-Blanco D., Reboiro-Jato M., Fdez-Riverola F., Posada D. ( 2010 ). alter: program-oriented conversion of DNA and protein alignments. . Nucleic Acids Res 38 (Suppl. 2), W14W18. [View Article] [PubMed]
    [Google Scholar]
  15. Hemberger H. ( 1982 ). Revision der Ordnung Hypotrichida Stein (Ciliophora, Protozoa) an Hand von Protargolpräparaten und Morphogenesedarstellungen. Dissertation:, Universität Bonn, Bonn, Germany (in German);.
    [Google Scholar]
  16. Hemberger H. ( 1985 ). Neue Gattungen und Arten hypotricher Ciliaten. . Arch Protistenkd 130, 397417 (in German). [View Article]
    [Google Scholar]
  17. Jankowski A. W. ( 1979 ). Revision of the order Hypotrichida Stein, 1859. Generic catalogue, phylogeny, taxonomy. . Trudy Zool Inst Leningrad 86, 4885 (in Russian).
    [Google Scholar]
  18. Kamra K., Sapra G. R. ( 1990 ). Partial retention of parental ciliature during morphogenesis of the ciliate Coniculostomum monilata (Dragesco & Njiné, 1971) Njiné, 1978 (Oxytrichidae, Hypotrichida). . Eur J Protistol 25, 264278. [View Article] [PubMed]
    [Google Scholar]
  19. Katoh K., Standley D. M. ( 2013 ). mafft multiple sequence alignment software version 7: improvements in performance and usability. . Mol Biol Evol 30, 772780. [View Article] [PubMed]
    [Google Scholar]
  20. Kumar S., Bharti D., Marinsalti S., Insom E., Terza A. L. ( 2014 ). Morphology, morphogenesis, and molecular phylogeny of Paraparentocirrus sibillinensis n. gen., n. sp., a “stylonychine Oxytrichidae” (Ciliophora, Hypotrichida) without transverse cirri. . J Eukaryot Microbiol 61, 247259. [View Article] [PubMed]
    [Google Scholar]
  21. Medlin L., Elwood H. J., Stickel S., Sogin M. L. ( 1988 ). The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. . Gene 71, 491499. [View Article] [PubMed]
    [Google Scholar]
  22. Paiva T. S., Silva-Neto I. D. ( 2007 ). Morphology and morphogenesis of Strongylidium pseudocrassum Wang and Nie, 1935, with redefinition of Strongylidium Sterki, 1878 (Protista: Ciliophora: Stichotrichia). . Zootaxa 1559, 3157.
    [Google Scholar]
  23. Posada D. ( 2008 ). jModelTest: phylogenetic model averaging. . Mol Biol Evol 25, 12531256. [View Article] [PubMed]
    [Google Scholar]
  24. Ronquist F., Huelsenbeck J. P. ( 2003 ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19, 15721574. [View Article] [PubMed]
    [Google Scholar]
  25. Sterki V. ( 1878 ). Beiträge zur Morphologie der Oxytrichinen. . Z Wiss Zool 31, 2958 (in German).
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  27. Tuffrau M. ( 1979 ). Une nouvelle famille d’hypotriches, Kahliellidae n. fam., et ses consequences dans la repartition des Stichotrichina. . Trans Am Microsc Soc 98, 521528 (in French). [View Article]
    [Google Scholar]
  28. Tuffrau M. ( 1987 ). Proposition d’une classification nouvelle de l’ordre Hypotrichida (Protozoa, Ciliophora), fondées récentes. . Ann Sci Nat (Zool) 13, 111117 (in French).
    [Google Scholar]
  29. Tuffrau M., Fleury A. ( 1994 ). Classe des Hypotrichea Stein, 1859. . In Traité de Zoologie , vol. II, issue 2, pp. 83151. Paris:: Editions Masson (in French);.
    [Google Scholar]
  30. Wang C. C., Nie D. ( 1935 ). Report on the rare and new species of freshwater infusoria, part II. . Sinensia 6, 399524 (in Chinese).
    [Google Scholar]
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