1887

Abstract

Two novel Gram-staining-positive, rod-shaped, endospore-forming and moderately thermophilic bacteria, designated strains DX-3 and GIESS002, were isolated from sludge composts from Guangdong Province, China. Analysis of 16S rRNA gene sequences revealed that the isolates were closely related to each other with extremely high similarity (99.6 %), and were members of the family . However, these two isolates formed a novel phylogenetic branch within this family. Their closest relatives were the members of the genera , and . Cells of both strains were facultatively anaerobic and catalase- and oxidase-positive. The cell-wall peptidoglycan type was A1γ (-diaminopimelic acid direct). The predominant isoprenoid quinone was MK-7. The main polar lipids were diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The major cellular fatty acid was iso-C. The DNA G+C content was 43.2–43.7 mol%. The results of a polyphasic taxonomic study indicated that strains DX-3 and GIESS002 represent a novel species in a new genus in the family , order , for which the name gen. nov., sp. nov. is proposed. The type strain is DX-3 ( = KCTC 33104 = CGMCC 1.12360).

Funding
This study was supported by the:
  • Agricultural Science & Technology Achievements Transfer Project of Guangdong Province, China (Award 2012NL040)
  • National Natural Science Foundation of China (Award 31100353)
  • Foundation for Excellent Young Scientist in Guangdong Academy of Sciences (Award qnjj201401)
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2015-02-01
2024-10-05
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References

  1. An S. Y., Asahara M., Goto K., Kasai H., Yokota A. ( 2007 ). Virgibacillus halophilus sp. nov., spore-forming bacteria isolated from soil in Japan. . Int J Syst Evol Microbiol 57, 16071611. [View Article] [PubMed]
    [Google Scholar]
  2. Bagheri M., Amoozegar M. A., Schumann P., Didari M., Mehrshad M., Spröer C., Sánchez-Porro C., Ventosa A. ( 2013 ). Ornithinibacillus halophilus sp. nov., a moderately halophilic, Gram-stain-positive, endospore-forming bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 63, 844848. [View Article] [PubMed]
    [Google Scholar]
  3. Baker G. C., Smith J. J., Cowan D. A. ( 2003 ). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55, 541555. [View Article] [PubMed]
    [Google Scholar]
  4. Carrasco I. J., Márquez M. C., Xue Y., Ma Y., Cowan D. A., Jones B. E., Grant W. D., Ventosa A. ( 2008 ). Sediminibacillus halophilus gen. nov., sp. nov., a moderately halophilic, Gram-positive bacterium from a hypersaline lake. . Int J Syst Evol Microbiol 58, 19611967. [View Article] [PubMed]
    [Google Scholar]
  5. Carrasco I. J., Márquez M. C., Ventosa A. ( 2009 ). Virgibacillus salinus sp. nov., a moderately halophilic bacterium from sediment of a saline lake. . Int J Syst Evol Microbiol 59, 30683073. [View Article] [PubMed]
    [Google Scholar]
  6. Cihan A. C., Ozcan B., Tekin N., Cokmus C. ( 2011 ). Geobacillus thermodenitrificans subsp. calidus, subsp. nov., a thermophilic and α-glucosidase producing bacterium isolated from Kizilcahamam, Turkey. . J Gen Appl Microbiol 57, 8392. [View Article] [PubMed]
    [Google Scholar]
  7. Dong X., Cai M. ( 2001 ). Manual of Systematic and Determinative Bacteriology. Beijing:: Academic Press;.
    [Google Scholar]
  8. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane-filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39, 224229. [View Article]
    [Google Scholar]
  9. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [View Article]
    [Google Scholar]
  10. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 11291133. [View Article] [PubMed]
    [Google Scholar]
  11. Han L., Yang G., Zhou X., Yang D., Hu P., Lu Q., Zhou S. ( 2013 ). Bacillus thermocopriae sp. nov., isolated from a compost. . Int J Syst Evol Microbiol 63, 30243029. [View Article] [PubMed]
    [Google Scholar]
  12. Kämpfer P., Falsen E., Lodders N., Langer S., Busse H. J., Schumann P. ( 2010 ). Ornithinibacillus contaminans sp. nov., an endospore-forming species. . Int J Syst Evol Microbiol 60, 29302934. [View Article] [PubMed]
    [Google Scholar]
  13. Kim Y. G., Choi D. H., Hyun S., Cho B. C. ( 2007 ). Oceanobacillus profundus sp. nov., isolated from a deep-sea sediment core. . Int J Syst Evol Microbiol 57, 409413. [View Article] [PubMed]
    [Google Scholar]
  14. L’Haridon S., Miroshnichenko M. L., Kostrikina N. A., Tindall B. J., Spring S., Schumann P., Stackebrandt E., Bonch-Osmolovskaya E. A., Jeanthon C. ( 2006 ). Vulcanibacillus modesticaldus gen. nov., sp. nov., a strictly anaerobic, nitrate-reducing bacterium from deep-sea hydrothermal vents. . Int J Syst Evol Microbiol 56, 10471053. [View Article] [PubMed]
    [Google Scholar]
  15. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. & other authors ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 21142121. [View Article] [PubMed]
    [Google Scholar]
  16. Mayr R., Busse H. J., Worliczek H. L., Ehling-Schulz M., Scherer S. ( 2006 ). Ornithinibacillus gen. nov., with the species Ornithinibacillus bavariensis sp. nov. and Ornithinibacillus californiensis sp. nov.. Int J Syst Evol Microbiol 56, 13831389. [View Article] [PubMed]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  18. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  19. Namwong S., Tanasupawat S., Lee K. C., Lee J. S. ( 2009 ). Oceanobacillus kapialis sp. nov., from fermented shrimp paste in Thailand. . Int J Syst Evol Microbiol 59, 22542259. [View Article] [PubMed]
    [Google Scholar]
  20. Nicolaus B., Lama L., Esposito E., Manca M. C., Di Prisco G., Gambacorta A. ( 1996 ). Bacillus thermoantarcticus” sp. nov., from Mount Melbourne, Antarctica: a novel thermophilic species. . Polar Biol 16, 101104.
    [Google Scholar]
  21. Nunes I., Tiago I., Pires A. L., da Costa M. S., Veríssimo A. ( 2006 ). Paucisalibacillus globulus gen. nov., sp. nov., a Gram-positive bacterium isolated from potting soil. . Int J Syst Evol Microbiol 56, 18411845. [View Article] [PubMed]
    [Google Scholar]
  22. Schumann P. ( 2011 ). Peptidoglycan structure. . Methods Microbiol 38, 101129. [View Article]
    [Google Scholar]
  23. Shin N. R., Whon T. W., Kim M. S., Roh S. W., Jung M. J., Kim Y. O., Bae J. W. ( 2012 ). Ornithinibacillus scapharcae sp. nov., isolated from a dead ark clam. . Antonie van Leeuwenhoek 101, 147154. [View Article] [PubMed]
    [Google Scholar]
  24. Smibert R. M., Krieg N. R. ( 1994 ). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R. . Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  25. Sung M. H., Kim H., Bae J. W., Rhee S. K., Jeon C. O., Kim K., Kim J. J., Hong S. P., Lee S. G. & other authors ( 2002 ). Geobacillus toebii sp. nov., a novel thermophilic bacterium isolated from hay compost. . Int J Syst Evol Microbiol 52, 22512255. [View Article] [PubMed]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  27. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  28. Xue Y., Zhang X., Zhou C., Zhao Y., Cowan D. A., Heaphy S., Grant W. D., Jones B. E., Ventosa A., Ma Y. ( 2006 ). Caldalkalibacillus thermarum gen. nov., sp. nov., a novel alkalithermophilic bacterium from a hot spring in China. . Int J Syst Evol Microbiol 56, 12171221. [View Article] [PubMed]
    [Google Scholar]
  29. Yang G., Chen M., Yu Z., Lu Q., Zhou S. ( 2013a ). Bacillus composti sp. nov. and Bacillus thermophilus sp. nov., two thermophilic, Fe(III)-reducing bacteria isolated from compost. . Int J Syst Evol Microbiol 63, 30303036. [View Article] [PubMed]
    [Google Scholar]
  30. Yang G., Zhou X., Zhou S., Yang D., Wang Y., Wang D. ( 2013b ). Bacillus thermotolerans sp. nov., a thermophilic bacterium capable of reducing humus. . Int J Syst Evol Microbiol 63, 36723678. [View Article] [PubMed]
    [Google Scholar]
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