%0 Journal Article %A Zhang, Dao-Feng %A Zhong, Jing-Mei %A Zhang, Xiao-Mei %A Jiang, Zhao %A Zhou, En-Min %A Tian, Xin-Peng %A Zhang, Si %A Li, Wen-Jun %T Nocardioidesnanhaiensis sp. nov., an actinobacterium isolated from a marine sediment sample %D 2014 %J International Journal of Systematic and Evolutionary Microbiology, %V 64 %N Pt_8 %P 2718-2722 %@ 1466-5034 %R https://doi.org/10.1099/ijs.0.062851-0 %I Microbiology Society, %X A rod- to coccus-shaped, non-spore-forming actinobacterium, strain YIM M13091T, was isolated from a marine sediment sample collected from the South China Sea and examined by a polyphasic approach to clarify its taxonomic position. This Gram-staining-positive, aerobic actinobacterium did not produce substrate mycelium and aerial hyphae, and no diffusible pigments were produced on the media tested. The optimum growth occurred at 30 °C, 1 % (w/v) NaCl and pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus Nocardioides , with low levels (≤96.2 %) of sequence similarity with respect to Nocardioides kribbensis KSL-2T and other members of the genus Nocardioides . Whole-organism hydrolysates of the strain contained ll-2,6-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H4), with MK-8 in a minor amount. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, hydroxyphosphatidylinositol and phosphatidylcholine, were the main polar lipids detected, while iso-C16 : 0 and C18 : 1ω9c were the major fatty acids. The G+C content of the genomic DNA was 68.5 mol%. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a member of the genus Nocardioides , and the name Nocardioides nanhaiensis sp. nov. (Type strain YIM M13091T = JCM 18127T = CCTCC AA 2011020T) is proposed for the novel species. %U https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijs.0.062851-0