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Abstract

A rod- to coccus-shaped, non-spore-forming actinobacterium, strain YIM M13091, was isolated from a marine sediment sample collected from the South China Sea and examined by a polyphasic approach to clarify its taxonomic position. This Gram-staining-positive, aerobic actinobacterium did not produce substrate mycelium and aerial hyphae, and no diffusible pigments were produced on the media tested. The optimum growth occurred at 30 °C, 1 % (w/v) NaCl and pH 8.0. Phylogenetic analysis based on 16S rRNA gene sequences showed that the isolate belongs to the genus , with low levels (≤96.2 %) of sequence similarity with respect to KSL-2 and other members of the genus . Whole-organism hydrolysates of the strain contained -2,6-diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinone was MK-8(H), with MK-8 in a minor amount. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, hydroxyphosphatidylinositol and phosphatidylcholine, were the main polar lipids detected, while iso-C and Cω9 were the major fatty acids. The G+C content of the genomic DNA was 68.5 mol%. Based on phylogenetic analysis, phenotypic and genotypic data, it is concluded that the isolate represents a member of the genus , and the name sp. nov. (Type strain YIM M13091 = JCM 18127 = CCTCC AA 2011020) is proposed for the novel species.

Funding
This study was supported by the:
  • , National Basic Research Program of China , (Award 2010CB833801)
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2014-08-01
2020-12-02
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