1887

Abstract

Five bacterial strains isolated from bats of the family Vespertilionidae were characterized by phenotypic tests and multilocus sequence analysis (MLSA) using the 16S rRNA gene and four housekeeping genes (, , , ). Phylogenetic analyses of individual and combined datasets indicated that the five strains represent a monophyletic cluster within the family . Comparison of 16S rRNA gene sequences demonstrated a high degree of similarity (98.3–99.9 %) among the group of bat-derived strains, while searches in nucleotide databases indicated less than 96 % sequence similarity to known members of the . The housekeeping genes , , and provided higher resolution compared with the 16S rRNA gene and subdivided the group according to the bat species from which the strains were isolated. Three strains derived from noctule bats shared 98.6–100 % sequence similarity in all four genes investigated, whereas, based on , and gene sequences, 91.8–96 % similarity was observed with and between the remaining two strains isolated from a serotine bat and a pipistrelle bat, respectively. Genome relatedness as deduced from gene sequences correlated well with the results of MLSA and indicated that the five strains represent a new genus. Based on these results, it is proposed to classify the five strains derived from bats within gen. nov., sp. nov. (the type species), genomospecies 1 and genomospecies 2. The genus can be distinguished phenotypically from recognized genera of the by at least three characteristics. All strains are nutritionally fastidious and require a chemically defined supplement with NAD for growth. The DNA G+C content of strain E127/08 is 38.2 mol%. The type strain of gen. nov., sp. nov. is E127/08 ( = CCUG 64585 = DSM 27238). The reference strains of genomospecies 1 and 2 are E145/08 and E157/08, respectively.

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2014-07-01
2019-10-16
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