1887

Abstract

We define two novel species of the genus that are phenotypically similar to and have near identical 16S rRNA gene sequences to . However, compared to and each other, the two species, sp. nov. (type strain MSHR1132 = DSM 28299 = SSI 89.005) and sp. nov. (type strain FSA084 = DSM 28300 = SSI 89.004), demonstrate: 1) at a whole-genome level considerable phylogenetic distance, lack of admixture, average nucleotide identity <95 %, and inferred DNA–DNA hybridization <70 %; 2) different profiles as determined by MALDI-TOF MS; 3) a non-pigmented phenotype for sp. nov.; 4) sp. nov. is not detected by standard PCR; 5) distinct peptidoglycan types compared to ; 6) a separate ecological niche for sp. nov.; and 7) a distinct clinical disease profile for sp. nov. compared to .

Funding
This study was supported by the:
  • , Wellcome Trust
  • , Deutsche Forschungsgemeinschaft , (Award EI 247/8-1)
  • , Australian National Health and Medical Research Council Career Development Fellow , (Award 1065736)
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2015-01-01
2021-03-08
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References

  1. Akobi B., Aboderin O., Sasaki T., Shittu A. ( 2012 ). Characterization of Staphylococcus aureus isolates from faecal samples of the Straw-Coloured Fruit Bat (Eidolon helvum) in Obafemi Awolowo University (OAU), Nigeria. . BMC Microbiol 12, 279. [CrossRef] [PubMed]
    [Google Scholar]
  2. Brakstad O. G., Aasbakk K., Maeland J. A. ( 1992 ). Detection of Staphylococcus aureus by polymerase chain reaction amplification of the nuc gene. . J Clin Microbiol 30, 16541660.[PubMed]
    [Google Scholar]
  3. Brennan L., Lilliebridge R. A., Cheng A. C., Giffard P. M., Currie B. J., Tong S. Y. ( 2013 ). Community-associated meticillin-resistant Staphylococcus aureus carriage in hospitalized patients in tropical northern Australia. . J Hosp Infect 83, 205211. [CrossRef] [PubMed]
    [Google Scholar]
  4. Cheng L., Connor T. R., Sirén J., Aanensen D. M., Corander J. ( 2013 ). Hierarchical and spatially explicit clustering of DNA sequences with BAPS software. . Mol Biol Evol 30, 12241228. [CrossRef] [PubMed]
    [Google Scholar]
  5. Goris J., Konstantinidis K. T., Klappenbach J. A., Coenye T., Vandamme P., Tiedje J. M. ( 2007 ). DNA-DNA hybridization values and their relationship to whole-genome sequence similarities. . Int J Syst Evol Microbiol 57, 8191. [CrossRef] [PubMed]
    [Google Scholar]
  6. Gotz F., Bannerman T., Schleifer K.-H. ( 2006 ). The genera Staphylococcus and Macrococcus . . In The Prokaryotes, 3rd edn, Vol. 4 Bacteria: Firmicutes, Cyanobacteria, pp. 5–75. Edited by Dworkin M., Falkow S., Rosenberg E., Schleifer K. H., Stackebrandt E. . New York, NY:: Springer;. [CrossRef]
    [Google Scholar]
  7. Holt D. C., Holden M. T., Tong S. Y., Castillo-Ramirez S., Clarke L., Quail M. A., Currie B. J., Parkhill J., Bentley S. D. et al. ( 2011 ). A very early-branching Staphylococcus aureus lineage lacking the carotenoid pigment staphyloxanthin. . Genome Biol Evol 3, 881895. [CrossRef] [PubMed]
    [Google Scholar]
  8. Jenney A., Holt D., Ritika R., Southwell P., Pravin S., Buadromo E., Carapetis J., Tong S., Steer A. ( 2014 ). The clinical and molecular epidemiology of Staphylococcus aureus infections in Fiji. . BMC Infect Dis 14, 160. [CrossRef] [PubMed]
    [Google Scholar]
  9. Kloos W. E., Schleifer K. H., Smith R. F. ( 1976 ). Characterization of Staphylococcus sciuri sp. nov. and its subspecies. . Int J Syst Bacteriol 26, 2237. [CrossRef]
    [Google Scholar]
  10. McDonald M., Dougall A., Holt D., Huygens F., Oppedisano F., Giffard P. M., Inman-Bamber J., Stephens A. J., Towers R. et al. ( 2006 ). Use of a single-nucleotide polymorphism genotyping system to demonstrate the unique epidemiology of methicillin-resistant Staphylococcus aureus in remote aboriginal communities. . J Clin Microbiol 44, 37203727. [CrossRef] [PubMed]
    [Google Scholar]
  11. Meier-Kolthoff J. P., Auch A. F., Klenk H. P., Göker M. ( 2013 ). Genome sequence-based species delimitation with confidence intervals and improved distance functions. . BMC Bioinformatics 14, 60. [CrossRef] [PubMed]
    [Google Scholar]
  12. Ng J. W., Holt D. C., Lilliebridge R. A., Stephens A. J., Huygens F., Tong S. Y., Currie B. J., Giffard P. M. ( 2009 ). Phylogenetically distinct Staphylococcus aureus lineage prevalent among indigenous communities in northern Australia. . J Clin Microbiol 47, 22952300. [CrossRef] [PubMed]
    [Google Scholar]
  13. Richter M., Rosselló-Móra R. ( 2009 ). Shifting the genomic gold standard for the prokaryotic species definition. . Proc Natl Acad Sci U S A 106, 1912619131. [CrossRef] [PubMed]
    [Google Scholar]
  14. Ruimy R., Angebault C., Djossou F., Dupont C., Epelboin L., Jarraud S., Lefevre L. A., Bes M., Lixandru B. E. et al. ( 2010 ). Are host genetics the predominant determinant of persistent nasal Staphylococcus aureus carriage in humans?. J Infect Dis 202, 924934. [CrossRef] [PubMed]
    [Google Scholar]
  15. Schaumburg F., Alabi A. S., Köck R., Mellmann A., Kremsner P. G., Boesch C., Becker K., Leendertz F. H., Peters G. ( 2012 ). Highly divergent Staphylococcus aureus isolates from African non-human primates. . Environ Microbiol Rep 4, 141146. [CrossRef] [PubMed]
    [Google Scholar]
  16. Schleifer K. H., Kandler O. ( 1972 ). Peptidoglycan types of bacterial cell walls and their taxonomic implications. . Bacteriol Rev 36, 407477.[PubMed]
    [Google Scholar]
  17. Schleifer K. H., Geyer U., Kilpper-Bälz R., Devriese L. A. ( 1983 ). Elevation of Staphylococcus sciuri subsp. lentus (Kloos et al.) to species status: Staphylococcus lentus (Kloos et al.) comb. nov.. Syst Appl Microbiol 4, 382387. [CrossRef] [PubMed]
    [Google Scholar]
  18. Schumann P. ( 2011 ). Peptidoglycan Structure. . Methods Microbiol 38, 101129.. [CrossRef]
    [Google Scholar]
  19. Švec P., Vancanneyt M., Sedláček I., Engelbeen K., Štětina V., Swings J., Petráš P. ( 2004 ). Reclassification of Staphylococcus pulvereri Zakrzewska-Czerwińska et al. 1995 as a later synonym of Staphylococcus vitulinus Webster et al. 1994. . Int J Syst Evol Microbiol 54, 22132215. [CrossRef] [PubMed]
    [Google Scholar]
  20. Tong S. Y., Lilliebridge R. A., Bishop E. J., Cheng A. C., Holt D. C., McDonald M. I., Giffard P. M., Currie B. J., Boutlis C. S. ( 2010 ). Clinical correlates of Panton-Valentine leukocidin (PVL), PVL isoforms, and clonal complex in the Staphylococcus aureus population of Northern Australia. . J Infect Dis 202, 760769. [CrossRef] [PubMed]
    [Google Scholar]
  21. Tong S. Y., Sharma-Kuinkel B. K., Thaden J. T., Whitney A. R., Yang S. J., Mishra N. N., Rude T., Lilliebridge R. A., Selim M. A. et al. ( 2013 ). Virulence of endemic nonpigmented northern Australian Staphylococcus aureus clone (clonal complex 75, S. argenteus) is not augmented by staphyloxanthin. . J Infect Dis 208, 520527. [CrossRef] [PubMed]
    [Google Scholar]
  22. Zakrzewska-Czerwińska J., Gaszewska-Mastalarz A., Lis B., Gamian A., Mordarski M. ( 1995 ). Staphylococcus pulvereri sp. nov., isolated from human and animal specimens. . Int J Syst Bacteriol 45, 169172. [CrossRef] [PubMed]
    [Google Scholar]
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