sp. nov., isolated from the coral Free

Abstract

A novel Gram-stain-positive actinobacterium, designated strain SCSIO 11529, was isolated from tissues of the stony coral , and characterized by using a polyphasic approach. The temperature range for growth was 22–50 °C (optimum 28–45 °C), the pH range for growth was 6.0–8.0 (optimum pH 7.0), and the NaCl concentration range for growth was 0–7 % (w/v) NaCl. The polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and an unknown polar lipid. The predominant menaquinone was MK-9(H). The major fatty acids (>10 %) were iso-C iso-Cω6, iso-C H and Cω7/iso-C 2-OH. The DNA G+C content of strain SCSIO 11529 was 70.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 11529 belongs to the genus , with the closest neighbours being MS498 (97.0 % 16S rRNA gene sequence similarity), DSM 43194 (96.4 %) and YIM 90630 (95.9 %). Based on the evidence of the present study, strain SCSIO 11529 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 11529 ( = DSM 45821 = NBRC 109418).

Funding
This study was supported by the:
  • National Basic Research Program of China (Award 2010CB833801)
  • National Natural Science Foundation of China (Award 41106139 and 41230962)
  • Pearl River Nova Program of Guangzhou (Award 2014J2200075)
  • Administration of Ocean and Fisheries of Guangdong Province (Award GD2012-D01-002)
  • Strategic Priority Research Program of the Chinese Academy of Sciences (Award XDA11030401)
  • Knowledge Innovation Program of the Chinese Academy of Sciences (Award SQ201301)
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2014-10-01
2024-03-29
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