1887

Abstract

A novel Gram-stain-positive actinobacterium, designated strain SCSIO 11529, was isolated from tissues of the stony coral , and characterized by using a polyphasic approach. The temperature range for growth was 22–50 °C (optimum 28–45 °C), the pH range for growth was 6.0–8.0 (optimum pH 7.0), and the NaCl concentration range for growth was 0–7 % (w/v) NaCl. The polar lipid profile contained diphosphatidylglycerol, phosphatidylcholine, phosphatidylglycerol, phosphatidylmethylethanolamine, phosphatidylethanolamine and an unknown polar lipid. The predominant menaquinone was MK-9(H). The major fatty acids (>10 %) were iso-C iso-Cω6, iso-C H and Cω7/iso-C 2-OH. The DNA G+C content of strain SCSIO 11529 was 70.2 mol%. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain SCSIO 11529 belongs to the genus , with the closest neighbours being MS498 (97.0 % 16S rRNA gene sequence similarity), DSM 43194 (96.4 %) and YIM 90630 (95.9 %). Based on the evidence of the present study, strain SCSIO 11529 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is SCSIO 11529 ( = DSM 45821 = NBRC 109418).

Funding
This study was supported by the:
  • , National Basic Research Program of China , (Award 2010CB833801)
  • , National Natural Science Foundation of China , (Award 41106139 and 41230962)
  • , Pearl River Nova Program of Guangzhou , (Award 2014J2200075)
  • , Administration of Ocean and Fisheries of Guangdong Province , (Award GD2012-D01-002)
  • , Strategic Priority Research Program of the Chinese Academy of Sciences , (Award XDA11030401)
  • , Knowledge Innovation Program of the Chinese Academy of Sciences , (Award SQ201301)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062679-0
2014-10-01
2020-09-21
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/10/3341.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062679-0&mimeType=html&fmt=ahah

References

  1. Collins M., Jones D. ( 1980 ). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2,4-diaminobutyric acid. . J Appl Bacteriol 48, 459470. [CrossRef]
    [Google Scholar]
  2. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221230. [CrossRef] [PubMed]
    [Google Scholar]
  3. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [CrossRef] [PubMed]
    [Google Scholar]
  4. Felsenstein J. ( 1985 ). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39, 783791. [CrossRef]
    [Google Scholar]
  5. Groth I., Schumann P., Rainey F. A., Martin K., Schuetze B., Augsten K. ( 1997 ). Demetria terragena gen. nov., sp. nov., a new genus of actinomycetes isolated from compost soil. . Int J Syst Bacteriol 47, 11291133. [CrossRef] [PubMed]
    [Google Scholar]
  6. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
    [Google Scholar]
  7. Kim S. B., Goodfellow M. ( 1999 ). Reclassification of Amycolatopsis rugosa Lechevalier et al. 1986 as Prauserella rugosa gen. nov., comb. nov.. Int J Syst Bacteriol 49, 507512. [CrossRef] [PubMed]
    [Google Scholar]
  8. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [CrossRef] [PubMed]
    [Google Scholar]
  9. Leifson E. ( 1960 ). Atlas of Bacterial Flagellation. London:: Academic Press;.
    [Google Scholar]
  10. Li W. J., Xu P., Tang S. K., Xu L. H., Kroppenstedt R. M., Stackebrandt E., Jiang C. L. ( 2003 ). Prauserella halophila sp. nov. and Prauserella alba sp. nov., moderately halophilic actinomycetes from saline soil. . Int J Syst Evol Microbiol 53, 15451549. [CrossRef] [PubMed]
    [Google Scholar]
  11. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L. ( 2007 ). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia . . Int J Syst Evol Microbiol 57, 14241428. [CrossRef] [PubMed]
    [Google Scholar]
  12. Li Y., Tang S. K., Chen Y. G., Wu J. Y., Zhi X. Y., Zhang Y. Q., Li W. J. ( 2009 ). Prauserella salsuginis sp. nov., Prauserella flava sp. nov., Prauserella aidingensis sp. nov. and Prauserella sediminis sp. nov., isolated from a salt lake. . Int J Syst Evol Microbiol 59, 29232928. [CrossRef] [PubMed]
    [Google Scholar]
  13. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [CrossRef]
    [Google Scholar]
  14. Minnikin D. E., Collins M. D., Goodfellow M. ( 1979 ). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47, 8795. [CrossRef]
    [Google Scholar]
  15. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  16. Schäfer J., Martin K., Kämpfer P. ( 2010 ). Prauserella muralis sp. nov., from the indoor environment. . Int J Syst Evol Microbiol 60, 287290. [CrossRef] [PubMed]
    [Google Scholar]
  17. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313340. [CrossRef]
    [Google Scholar]
  18. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [CrossRef] [PubMed]
    [Google Scholar]
  19. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [CrossRef] [PubMed]
    [Google Scholar]
  20. Wang J., Li Y., Bian J., Tang S. K., Ren B., Chen M., Li W. J., Zhang L. X. ( 2010 ). Prauserella marina sp. nov., isolated from ocean sediment of the South China Sea. . Int J Syst Evol Microbiol 60, 985989. [CrossRef] [PubMed]
    [Google Scholar]
  21. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L. ( 2005 ). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55, 11491153. [CrossRef] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062679-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062679-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error