1887

Abstract

A thermotolerant, Gram-strain-negative, non-spore-forming and strictly aerobic bacterium, designated GU51, was isolated from Guhai hot spring in Jimsar county, Xinjiang province, north-west China. Each cell of strain GU51 consisted of an oval body and two symmetrical long (3–6 µm) prosthecae. The strain moved by polar flagellum. Oxidase and catalase were produced. Strain GU51 grew within the ranges of 37–65 °C (optimum 48–50 °C), 0.5–7.5 % (w/v) NaCl (optimum 2–3 %) and pH 6.0–9.0 (optimum pH 7.5). The major respiratory quinone detected was ubiquinone 10 (U-10) and the genomic DNA G+C content was 66.7±0.4 mol%. Major fatty acids (>5 %) were C, Cω7 and 11-methyl Cω7. The polar lipids consisted of diphosphatidylglycerol, five glycolipids, phosphatidylglycerol and an unknown phospholipid. Phylogenetic analysis showed the closest relatives of strain GU51 were members of the genus with 92.3 % 16S rRNA gene sequence similarity. On the basis of this polyphasic taxonomic characterization, it is suggested that strain GU51 represents a novel species of a new genus in the family ‘’, for which the name gen. nov., sp. nov. is proposed. The type strain of the type species is GU51 ( = CGMCC 1.12710 = JCM 19779).

Funding
This study was supported by the:
  • Opening Project of the Key Laboratory of Biogeography and Bioresources in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences (Award LBB-2011-005)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062471-0
2014-08-01
2022-06-25
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/8/2805.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062471-0&mimeType=html&fmt=ahah

References

  1. Arun A. B., Chen W.-M., Lai W.-A., Chou J.-H., Rekha P. D., Shen F.-T., Singh S., Young C.-C. ( 2009 ). Parvularcula lutaonensis sp. nov., a moderately thermotolerant marine bacterium isolated from a coastal hot spring. . Int J Syst Evol Microbiol 59, 9981001. [View Article] [PubMed]
    [Google Scholar]
  2. Caumette P., Guyoneaud R., Duran R., Cravo-Laureau C., Matheron R. ( 2007 ). Rhodobium pfennigii sp. nov., a phototrophic purple non-sulfur bacterium with unusual bacteriochlorophyll a antennae, isolated from a brackish microbial mat on Rangiroa atoll, French Polynesia. . Int J Syst Evol Microbiol 57, 12501255. [View Article] [PubMed]
    [Google Scholar]
  3. Cho J.-C., Giovannoni S. J. ( 2003 ). Parvularcula bermudensis gen. nov., sp. nov., a marine bacterium that forms a deep branch in the α-Proteobacteria . . Int J Syst Evol Microbiol 53, 10311036. [View Article] [PubMed]
    [Google Scholar]
  4. Cui H. L., Yang X., Gao X., Xu X. W. ( 2011 ). Halobellus clavatus gen. nov., sp. nov. and Halorientalis regularis gen. nov., sp. nov., two new members of the family Halobacteriaceae . . Int J Syst Evol Microbiol 61, 26822689. [View Article] [PubMed]
    [Google Scholar]
  5. Dong X. Z., Cai M. Y. ( 2001 ). Determinative Manual for Routine Bacteriology. Beijing:: Scientific Press (English translation);.
    [Google Scholar]
  6. Doronina N. V., Poroshina M. N., Kaparullina E. N., Ezhov V. A., Trotsenko Y. A. ( 2013 ). Methyloligella halotolerans gen. nov., sp. nov. and Methyloligella solikamskensis sp. nov., two non-pigmented halotolerant obligately methylotrophic bacteria isolated from the Ural saline environments. . Syst Appl Microbiol 36, 148154. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368376. [View Article] [PubMed]
    [Google Scholar]
  8. Hungate R. E. ( 1969 ). A roll tube method for the cultivation of strict anaerobes. . Methods Microbiol 3B, 117132. [View Article]
    [Google Scholar]
  9. Jarvis B. D. W., Pankhurst C. E., Patel J. J. ( 1982 ). Rhizobium loti, a new species of legume root nodule bacteria. . Int J Syst Bacteriol 32, 378380. [View Article]
    [Google Scholar]
  10. Jarvis B. D. W., Van Berkum P., Chen W. X., Nour S. M., Fernandez M. P., Cleyet-Marel J. C., Gillis M. ( 1997 ). Transfer of Rhizobium loti, Rhizobium huakuii, Rhizobium ciceri, Rhizobium mediterraneum, and Rhizobium tianshanense to Mesorhizobium gen. nov.. Int J Syst Bacteriol 47, 895898. [View Article]
    [Google Scholar]
  11. Kanso S., Patel B. K. C. ( 2004 ). Phenylobacterium lituiforme sp. nov., a moderately thermophilic bacterium from a subsurface aquifer, and emended description of the genus Phenylobacterium . . Int J Syst Evol Microbiol 54, 21412146. [View Article] [PubMed]
    [Google Scholar]
  12. Kates M. ( 1986 ). Techniques of Lipidology, , 2nd edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  13. Kawasaki K., Nogi Y., Hishinuma M., Nodasaka Y., Matsuyama H., Yumoto I. ( 2002 ). Psychromonas marina sp. nov., a novel halophilic, facultatively psychrophilic bacterium isolated from the coast of the Okhotsk Sea. . Int J Syst Evol Microbiol 52, 14551459. [View Article] [PubMed]
    [Google Scholar]
  14. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  15. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111120. [View Article] [PubMed]
    [Google Scholar]
  16. Liu J.-J., Zhang X.-Q., Chi F.-T., Pan J., Sun C., Wu M. ( 2014 ). Gemmobacter megaterium sp. nov., isolated from coastal planktonic seaweeds. . Int J Syst Evol Microbiol 64, 6671. [View Article] [PubMed]
    [Google Scholar]
  17. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from micro-organisms. . J Mol Biol 3, 208218. [View Article]
    [Google Scholar]
  18. Mata J. A., Martínez-Cánovas J., Quesada E., Béjar V. ( 2002 ). A detailed phenotypic characterisation of the type strains of Halomonas species. . Syst Appl Microbiol 25, 360375. [View Article] [PubMed]
    [Google Scholar]
  19. Mesbah M., Whitman W. B. ( 1989 ). Measurement of deoxyguanosine/thymidine ratios in complex mixtures by high-performance liquid chromatography for determination of the mole percentage guanine + cytosine of DNA. . J Chromatogr A 479, 297306. [View Article] [PubMed]
    [Google Scholar]
  20. Nokhal T.-H., Schlegel H. G. ( 1983 ). Taxonomic study of Paracoccus denitrificans . . Int J Syst Bacteriol 33, 2637. [View Article]
    [Google Scholar]
  21. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  22. Shen P., Chen X.-D. ( 2008 ) . Experiment of Microbiology. . Beijing:: Higher Education Press (English translation);.
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  24. Urdiain M., López-López A., Gonzalo C., Busse H. J., Langer S., Kämpfer P., Rosselló-Móra R. ( 2008 ). Reclassification of Rhodobium marinum and Rhodobium pfennigii as Afifella marina gen. nov. comb. nov. and Afifella pfennigii comb. nov., a new genus of photoheterotrophic Alphaproteobacteria and emended descriptions of Rhodobium, Rhodobium orientis and Rhodobium gokarnense . . Syst Appl Microbiol 31, 339351. [View Article] [PubMed]
    [Google Scholar]
  25. Xu X.-W., Wu Y.-H., Zhou Z., Wang C.-S., Zhou Y.-G., Zhang H.-B., Wang Y., Wu M. ( 2007 ). Halomonas saccharevitans sp. nov., Halomonas arcis sp. nov. and Halomonas subterranea sp. nov., halophilic bacteria isolated from hypersaline environments of China. . Int J Syst Evol Microbiol 57, 16191624. [View Article] [PubMed]
    [Google Scholar]
  26. Yabe S., Aiba Y., Sakai Y., Hazaka M., Yokota A. ( 2012 ). Thermovum composti gen. nov., sp. nov., an alphaproteobacterium from compost. . Int J Syst Evol Microbiol 62, 29912996. [View Article] [PubMed]
    [Google Scholar]
  27. Yu Z., Lai Q., Li G., Shao Z. ( 2013 ). Parvularcula dongshanensis sp. nov., isolated from soft coral. . Int J Syst Evol Microbiol 63, 21142117. [View Article] [PubMed]
    [Google Scholar]
  28. Zhang X.-Q., Ying Y., Ye Y., Xu X. W., Zhu X. F., Wu M. ( 2010 ). Thermus arciformis sp. nov., a thermophilic species from a geothermal area. . Int J Syst Evol Microbiol 60, 834839. [View Article] [PubMed]
    [Google Scholar]
  29. Zhang X.-Q., Zhang Z.-L., Wu N., Zhu X.-F., Wu M. ( 2013 ). Anoxybacillus vitaminiphilus sp. nov., a strictly aerobic and moderately thermophilic bacterium isolated from a hot spring. . Int J Syst Evol Microbiol 63, 40644071. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062471-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062471-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error