1887

Abstract

Strain MUSC 115 was isolated from mangrove soil of the Tanjung Lumpur river in the state of Pahang, Peninsular Malaysia. Cells of this strain stained Gram-positive and were non-spore-forming, short rods that formed yellowish-white colonies on different agar media. The taxonomy of strain MUSC 115 was studied by a polyphasic approach, and the organism showed a range of phylogenetic and chemotaxonomic properties consistent with those of the genus . The cell-wall peptidoglycan was of type B2β, containing the amino acids ornithine, alanine, glycine, glutamic acid and homoserine. The muramic acid was of the -glycolyl form. The predominant menaquinones detected were MK-12, MK-13 and MK-11. The polar lipids consisted of phosphatidylglycerol, phosphoglycolipid, diphosphatidylglycerol, two unidentified lipids, three unidentified phospholipids and four unidentified glycolipids. The major fatty acids of the cell membrane were anteiso-C and anteiso-C. The whole-cell sugars detected were ribose, glucose, mannose and galactose. Based on the 16S rRNA gene sequence, strain MUSC 115 showed the highest sequence similarity to SK 18 (98.1 %), XIL02 (97.8 %) and DSM 20754 (97.5 %) and lower sequence similarity to strains of other species of the genus . DNA–DNA hybridization experiments revealed a low level of DNA–DNA relatedness (less than 24 %) between strain MUSC 115 and the type strains of closely related species. Furthermore, BOX-PCR fingerprint comparison also indicated that strain MUSC 115 represented a unique DNA profile. The DNA G+C content determined was 70.9±0.7 mol%, which is lower than that of SK 18. Based on the combination of genotypic and phenotypic data, it is proposed that strain MUSC 115 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is MUSC 115 ( = MCCC 1K00251 = DSM 28240 = NBRC 110089).

Funding
This study was supported by the:
  • University of Malaya for High Impact Research (Award External Industry Grant (vote no. GBA-808138) and UM-MOHE HIR Nature Microbiome grant no. H-50001-A000027))
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2014-10-01
2024-03-29
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