1887

Abstract

A novel species of basidiomycetes was isolated from kitchen garden soil in Shahryar city, Tehran province, Iran. Molecular and conventional methods were employed to identify and classify this single isolate. Morphologically, the isolate was considered yeast-like with hyaline and oval cells reproducing by monopolar budding, forming ballistoconidia, hyphae, arthroconidia and didymospores. Basidia and basidiospores resembling those produced by species were observed. Sequencing and Bayesian phylogenetic analysis of rRNA genes and the internal transcribed spacer region revealed its sister relationship to described species of the genus . Assimilation and fermentation tests, cell-wall carbohydrate analysis and enzyme activity tests were performed to provide insight into the metabolism of the isolate. Based on morphology, physiology and phylogeny of rRNA gene sequences, the isolate was shown to represent a novel species of the genus , described as sp. nov. (holotype IBRC P1010180 = ex-type IBRC M30078 = isotype CBS 12808). The MycoBank number of the novel species is MB 804703. An emended description of the genus is also provided.

Funding
This study was supported by the:
  • Iranian Biological Resource Center (IBRC), ACECR
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062380-0
2014-09-01
2021-10-23
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/9/3046.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062380-0&mimeType=html&fmt=ahah

References

  1. Anderson I. C., Cairney J. W. ( 2004 ). Diversity and ecology of soil fungal communities: increased understanding through the application of molecular techniques. . Environ Microbiol 6, 769779. [View Article] [PubMed]
    [Google Scholar]
  2. Barnett J. A., Payne R. W., Yarrow D. ( 2000 ). Yeasts: Characteristics and Identification, , 3rd edn.. Cambridge:: Cambridge University Press;.
    [Google Scholar]
  3. Blackwell M. ( 2011 ). The fungi: 1, 2, 3 ... 5.1 million species?. Am J Bot 98, 426438. [View Article] [PubMed]
    [Google Scholar]
  4. Boekhout T., Theelen B., Bai F., Wang Q., Liu X. ( 2012 ). Towards a unifying classification of basidiomycetous yeasts. 13th International Congress on Yeasts. . Wisconsin;, USA;.
    [Google Scholar]
  5. Botha A. ( 2011 ). The importance and ecology of yeasts in soil. . Soil Biol Biochem 43, 18. [View Article]
    [Google Scholar]
  6. Bresolin J. D., Bustamante M. M. C., Krüger R. H., Silva M. R. S. S., Perez K. S. ( 2010 ). Structure and composition of bacterial and fungal community in soil under soybean monoculture in the Brazilian Cerrado. . Braz J Microbiol 41, 391403. [View Article] [PubMed]
    [Google Scholar]
  7. Brizzio S., Turchetti B., de García V., Libkind D., Buzzini P., van Broock M. ( 2007 ). Extracellular enzymatic activities of basidiomycetous yeasts isolated from glacial and subglacial waters of northwest Patagonia (Argentina). . Can J Microbiol 53, 519525. [View Article] [PubMed]
    [Google Scholar]
  8. Carvalho F. P., de Souza A. C., Magalhães-Guedes K. T., Dias D. R., Silva C. F., Schwan R. F. ( 2013 ). Yeasts diversity in Brazilian Cerrado soils: study of the enzymatic activities. . Afr J Microbiol Res 7, 41764190. [View Article]
    [Google Scholar]
  9. Dörfler C. ( 1990 ). Vergleichende untersuchungen zum biochemischen Aufbu der Zellwand an Hefestadien von niederen und höheren Basidiomyceten. (Bibliotheca Mycologica 129), pp1163. Berlin:: J. Cramer;.
    [Google Scholar]
  10. Jones E. B. G, Pang K.-L. ( 2012 ). Tropical aquatic fungi. . Biodivers Conserv 21, 24032423. [View Article]
    [Google Scholar]
  11. Katoh K., Kuma K., Toh H., Miyata T. ( 2005 ). mafft version 5: improvement in accuracy of multiple sequence alignment. . Nucleic Acids Res 33, 511518. [View Article] [PubMed]
    [Google Scholar]
  12. Komagata K., Suzuki K. ( 1987 ). Lipid and cell-wall analysis in bacterial systematics. . Methods Microbiol 19, 161207. [View Article]
    [Google Scholar]
  13. Kurtzman C. P. ( 2011 ). Recognition of yeast species from gene sequence comparisons. . Open Appl Informat J 5, 2029. [View Article]
    [Google Scholar]
  14. Kurtzman C. P., Robnett C. J. ( 1998 ). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. . Antonie van Leeuwenhoek 73, 331371. [View Article] [PubMed]
    [Google Scholar]
  15. Kurtzman C. P., Fell J. W., Boekhout T. ( 2011 ). The Yeasts, A Taxonomic Study, , 5th edn.. Amsterdam:: Elsevier;.
    [Google Scholar]
  16. Li A., Zhu Y., Xu L., Zhu W., Tian X. ( 2008 ). Comparative study on the determination of assay for laccase of Trametes sp.. Afr J Biochem Res 2, 181183.
    [Google Scholar]
  17. Limtong S., Yongmanitchai W., Tun M. M., Kawasaki H., Seki T. ( 2007 ). Kazachstania siamensis sp. nov., an ascomycetous yeast species from forest soil in Thailand. . Int J Syst Evol Microbiol 57, 419422. [View Article] [PubMed]
    [Google Scholar]
  18. Lin D., Wu L. C., Rinaldi M. G., Lehmann P. F. ( 1995 ). Three distinct genotypes within Candida parapsilosis from clinical sources. . J Clin Microbiol 33, 18151821.[PubMed]
    [Google Scholar]
  19. Matsushima T. ( 2001 ). Matsushima Mycological Memoirs, vol. 10. Kobe:: CD-ROM, published by the author.
    [Google Scholar]
  20. Mikita K., Yamada Y. ( 1999 ). The ubiquinone system in Hasegawaea japonica (Yukawa et Maki) Yamada et Banno: a new method for identifying ubiquinone homologs from yeast cells. . IFO Res Commun 19, 4146.
    [Google Scholar]
  21. Nguyen H. D. T., Nickerson N. L., Seifert K. A. ( 2013 ). Basidioascus and Geminibasidium: a new lineage of heat-resistant and xerotolerant basidiomycetes. . Mycologia 105, 12311250. [View Article] [PubMed]
    [Google Scholar]
  22. Olsen I. ( 1990 ). Chemotaxonomy of yeasts. . Acta Odontol Scand 48, 1925. [View Article] [PubMed]
    [Google Scholar]
  23. Ronquist F., Huelsenbeck J. P. ( 2003 ). MrBayes 3: Bayesian phylogenetic inference under mixed models. . Bioinformatics 19, 15721574. [View Article] [PubMed]
    [Google Scholar]
  24. Valente P., Boekhout T., Landell M. F., Crestani J., Pagnocca F. C., Sette L. D., Passarini M. R., Rosa C. A., Brandão L. R. & other authors ( 2012 ). Bandoniozyma gen. nov., a genus of fermentative and non-fermentative tremellaceous yeast species. . PLoS ONE 7, e46060. [View Article] [PubMed]
    [Google Scholar]
  25. White T. J., Bruns T., Lee S., Taylor J. W. ( 1990 ). Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. . In PCR Protocols: a Guide to Methods and Amplifications, pp. 315322. Edited by Innis M. A., Gelfand D. H., Sninsky J. J., White T. J. . New York:: Academic Press;.
    [Google Scholar]
  26. Yurkov A. M., Kemler M., Begerow D. ( 2012 ). Assessment of yeast diversity in soils under different management regimes. . Fungal Ecol 5, 2435. [View Article]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062380-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062380-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF

Most cited this month Most Cited RSS feed

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error