1887

Abstract

A novel Gram-stain-positive, motile, catalase- and oxidase-positive, aerobic, endospore-forming, peritrichous, rod-shaped bacterium, designated YIM M13235, was isolated from a marine sediment sample collected from the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M13235 belonged to the genus . The strain grew optimally at 30 °C, pH 7.0 and in the presence of 2–4 % (w/v) NaCl. -Diaminopimelic acid was present in the cell-wall peptidoglycan. Strain YIM M13235 exhibited a menaquinone system with MK-7, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unknown phospholipids and one unknown glycolipid. The major fatty acids (>5 %) were iso-C, anteiso-C, anteiso-C, iso-Cω10 and summed feature 4 (anteiso-C and/or iso-C). The genomic DNA G+C content was 42.1 mol%. The DNA–DNA relatedness values between strain YIM M13235 and its close relatives (16S rRNA gene sequence similarities >97 %) including DSM 8723, DSM 8719 and JSM 099021 were 41 %, 44 % and 44 %, respectively. On the basis of genotypic, phenotypic and DNA–DNA relatedness data, it is apparent that strain YIM M13235 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM M13235 ( = DSM 25879 = KCTC 33044).

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2014-06-01
2019-12-15
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References

  1. Ash C., Farrow J. A. E., Wallbanks S., Collins M. D.. ( 1991;). Phylogenetic heterogeneity of the genus Bacillus revealed by comparative analysis of small-subunit ribosomal RNA sequences. . Lett Appl Microbiol 13:, 202–206. [CrossRef]
    [Google Scholar]
  2. Chen Y. G., Hu S. P., Tang S. K., He J. W., Xiao J. Q., Zhu H. Y., Li W. J.. ( 2011;). Bacillus zhanjiangensis sp. nov., isolated from an oyster in South China Sea. . Antonie van Leeuwenhoek 99:, 473–480. [CrossRef][PubMed]
    [Google Scholar]
  3. Cohen-Bazire G., Sistrom W. R., Stanier R. Y.. ( 1957;). Kinetic studies of pigment synthesis by non-sulfur purple bacteria. . J Cell Comp Physiol 49:, 25–68. [CrossRef][PubMed]
    [Google Scholar]
  4. Collins M. D., Jones D.. ( 1980;). Lipids in the classification and identification of coryneform bacteria containing peptidoglycans based on 2, 4-diaminobutyric acid. . J Appl Bacteriol 48:, 459–470. [CrossRef]
    [Google Scholar]
  5. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E.. ( 1977;). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100:, 221–230. [CrossRef][PubMed]
    [Google Scholar]
  6. Cowan S. T., Steel K. J.. ( 1965;). Manual for the Identification of Medical Bacteria. London:: Cambridge University Press;.
    [Google Scholar]
  7. Ezaki T., Hashimoto Y., Yabuuchi E.. ( 1989;). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Bacteriol 39:, 224–229. [CrossRef]
    [Google Scholar]
  8. Felsenstein J.. ( 1981;). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17:, 368–376. [CrossRef][PubMed]
    [Google Scholar]
  9. Felsenstein J.. ( 1985;). Confidence limits on phylogenies: an approach using the bootstrap. . Evolution 39:, 783–791. [CrossRef]
    [Google Scholar]
  10. Fitch W. M.. ( 1971;). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20:, 406–416. [CrossRef]
    [Google Scholar]
  11. Gordon R. E., Barnett D. A., Handerhan J. E., Pang C. H.-N.. ( 1974;). Nocardia coeliaca, Nocardia autotrophica and the nocardin strain. . Int J Syst Bacteriol 24:, 54–63. [CrossRef]
    [Google Scholar]
  12. Jiang Y., Tang S. K., Wiese J., Xu L. H., Imhoff J. F., Jiang C. L.. ( 2007;). Streptomyces hainanensis sp. nov., a novel member of the genus Streptomyces. . Int J Syst Evol Microbiol 57:, 2694–2698. [CrossRef][PubMed]
    [Google Scholar]
  13. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  14. Kimura M.. ( 1980;). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16:, 111–120. [CrossRef][PubMed]
    [Google Scholar]
  15. Li W. J., Xu P., Schumann P., Zhang Y. Q., Pukall R., Xu L. H., Stackebrandt E., Jiang C. L.. ( 2007;). Georgenia ruanii sp. nov., a novel actinobacterium isolated from forest soil in Yunnan (China), and emended description of the genus Georgenia. . Int J Syst Evol Microbiol 57:, 1424–1428. [CrossRef][PubMed]
    [Google Scholar]
  16. Marmur J.. ( 1961;). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3:, 208–218. [CrossRef]
    [Google Scholar]
  17. Minnikin D. E., Collins M. D., Goodfellow M.. ( 1979;). Fatty acid and polar lipid composition in the classification of Cellulomonas, Oerskovia and related taxa. . J Appl Bacteriol 47:, 87–95. [CrossRef]
    [Google Scholar]
  18. Nielsen P., Fritze D., Priest F. G.. ( 1995;). Phenetic diversity of alkaliphilic Bacillus strains: proposal for nine new species. . Microbiology 141:, 1745–1761. [CrossRef]
    [Google Scholar]
  19. Saitou N., Nei M.. ( 1987;). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4:, 406–425.[PubMed]
    [Google Scholar]
  20. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–654. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  21. Spanka R., Fritze D.. ( 1993;). Bacillus cohnii sp. nov., a new, obligately alkaliphilic, oval-spore-forming Bacillus species with ornithine and aspartic acid instead of diaminopimelic acid in the cell wall. . Int J Syst Bacteriol 43:, 150–156. [CrossRef][PubMed]
    [Google Scholar]
  22. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA–DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  23. Stackebrandt E., Liesack W.. ( 1993;). Nucleic acids and classification. . In Handbook of New Bacterial Systematics, pp. 152–189. Edited by Goodfellow M., O’Donnell A. G... London:: Academic Press;.
    [Google Scholar]
  24. Staneck J. L., Roberts G. D.. ( 1974;). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28:, 226–231.[PubMed]
    [Google Scholar]
  25. Tamaoka J., Katayama-Fujimura Y., Kuraishi H.. ( 1983;). Analysis of bacterial menaquinone mixtures by high performance liquid chromatography. . J Appl Bacteriol 54:, 31–36. [CrossRef]
    [Google Scholar]
  26. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S.. ( 2011;). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28:, 2731–2739. [CrossRef][PubMed]
    [Google Scholar]
  27. Turnbull P. C. B.. ( 1996;). Bacillus. . In: Barron's Medical Microbiology ( Baron S. et al., eds.) (, 4th ed..). Univ of Texas Medical Branch;. ISBN 978-0-9631172-1-2.
    [Google Scholar]
  28. Van Ert M., Staley J. T.. ( 1971;). Gas-vacuolated strains of Microcyclus aquaticus. . J Bacteriol 108:, 236–240.[PubMed]
    [Google Scholar]
  29. Ventosa A., Nieto J. J., Oren A.. ( 1998;). Biology of moderately halophilic aerobic bacteria. . Microbiol Mol Biol Rev 62:, 504–544.[PubMed]
    [Google Scholar]
  30. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E.. & other authors ( 1987;). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37:, 463–464. [CrossRef]
    [Google Scholar]
  31. Xu P., Li W. J., Tang S. K., Zhang Y. Q., Chen G. Z., Chen H. H., Xu L. H., Jiang C. L.. ( 2005;). Naxibacter alkalitolerans gen. nov., sp. nov., a novel member of the family ‘Oxalobacteraceae’ isolated from China. . Int J Syst Evol Microbiol 55:, 1149–1153. [CrossRef][PubMed]
    [Google Scholar]
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