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Abstract

A novel Gram-stain-positive, motile, catalase- and oxidase-positive, aerobic, endospore-forming, peritrichous, rod-shaped bacterium, designated YIM M13235, was isolated from a marine sediment sample collected from the South China Sea. Phylogenetic analysis based on 16S rRNA gene sequences showed that strain YIM M13235 belonged to the genus . The strain grew optimally at 30 °C, pH 7.0 and in the presence of 2–4 % (w/v) NaCl. -Diaminopimelic acid was present in the cell-wall peptidoglycan. Strain YIM M13235 exhibited a menaquinone system with MK-7, and the major polar lipids consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, four unknown phospholipids and one unknown glycolipid. The major fatty acids (>5 %) were iso-C, anteiso-C, anteiso-C, iso-Cω10 and summed feature 4 (anteiso-C and/or iso-C). The genomic DNA G+C content was 42.1 mol%. The DNA–DNA relatedness values between strain YIM M13235 and its close relatives (16S rRNA gene sequence similarities >97 %) including DSM 8723, DSM 8719 and JSM 099021 were 41 %, 44 % and 44 %, respectively. On the basis of genotypic, phenotypic and DNA–DNA relatedness data, it is apparent that strain YIM M13235 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is YIM M13235 ( = DSM 25879 = KCTC 33044).

Funding
This study was supported by the:
  • , National Basic Research Program of China , (Award 2010CB833801)
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2014-06-01
2020-10-24
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