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Volume 64, Issue Pt_9

sp. nov. and sp. nov., two halotolerant actinomycetes isolated from a salt lake and saltern Free

Abstract

The taxonomic positions of two novel actinomycetes, designated strains BNT558 and SM3501, were established by using a polyphasic approach. The organisms had chemical and morphological features that were consistent with their classification in the genus . The whole-cell hydrolysates of the two strains contained -diaminopimelic acid as the diagnostic diamino acid. The predominant menaquinones were MK-9(H) and MK-9(H) for strain BNT558 and MK-9(H) and MK-9(H) for strain SM3501. Major fatty acids of the strains were anteiso-C, anteiso-C and iso-C. The polar lipid profile of strain BNT558 contained diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylinositol, one unidentified glycolipid and one unidentified aminophospholipid, while that of strain SM3501 consisted of diphosphatidylglycerol, phosphatidylglycerol, phosphatidylinositol, phosphatidylethanolamine, three unidentified atypical aminolipids, one unidentified aminolipid and two unidentified glycolipids. The G+C contents of the genomic DNA were 70.2 and 69.6 mol% for strains BNT558 and SM3501, respectively. 16S rRNA gene sequence data supported the classification of the isolates in the genus and showed that they formed two distinct branches within the genus. Based on almost-complete 16S rRNA gene sequences, strain BNT558 was related most closely to NRRL B-24327 and strain SM3501 was related most closely to NBRC 12748. DNA–DNA relatedness between each of the isolates and its closest phylogenetic neighbours showed that they belonged to distinct species. The two isolates were readily distinguished from one another and from the type strains of the other species classified in the genus based on a combination of phenotypic and genotypic properties. Based on the genotypic and phenotypic evidence, strains BNT558 and SM3501 belong to two novel species in the genus , for which the names sp. nov. (type strain BNT558 = KCTC 29198 = DSM 42109) and p. nov. (type strain SM3501 = KCTC 29214 = DSM 42105) are proposed, respectively.

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Funding
This study was supported by the:
  • Anadolu University (Award 1201F012)
© 2014 IUMS
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2014-09-01
2024-04-20
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References

  1. Antón J., Oren A., Benlloch S., Rodríguez-Valera F., Amann R., Rosselló-Mora R. ( 2002 ). Salinibacter ruber gen. nov., sp. nov., a novel, extremely halophilic member of the Bacteria from saltern crystallizer ponds. . Int J Syst Evol Microbiol 52, 485–491.[PubMed]
    [Google Scholar]
  2. Cashion P., Holder-Franklin M. A., McCully J., Franklin M. ( 1977 ). A rapid method for the base ratio determination of bacterial DNA. . Anal Biochem 81, 461–466. [View Article] [PubMed]
    [Google Scholar]
  3. Chun J., Goodfellow M. ( 1995 ). A phylogenetic analysis of the genus Nocardia with 16S rRNA gene sequences. . Int J Syst Bacteriol 45, 240–245. [View Article] [PubMed]
    [Google Scholar]
  4. Collins M. D., Pirouz T., Goodfellow M., Minnikin D. E. ( 1977 ). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol 100, 221–230. [View Article] [PubMed]
    [Google Scholar]
  5. De Ley J., Cattoir H., Reynaerts A. ( 1970 ). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem 12, 133–142. [View Article] [PubMed]
    [Google Scholar]
  6. Felsenstein J. ( 1981 ). Evolutionary trees from DNA sequences: a maximum likelihood approach. . J Mol Evol 17, 368–376. [View Article] [PubMed]
    [Google Scholar]
  7. Felsenstein J. ( 1985 ). Confidence limits on phylogeny: an approach using the bootstrap. . Evolution 39, 783–791. [View Article]
    [Google Scholar]
  8. Fiedler H.-P., Bruntner C., Bull A. T., Ward A. C., Goodfellow M., Potterat O., Puder C., Mihm G. ( 2005 ). Marine actinomycetes as a source of novel secondary metabolites. . Antonie van Leeuwenhoek 87, 37–42. [View Article] [PubMed]
    [Google Scholar]
  9. Fitch W. M. ( 1971 ). Toward defining the course of evolution: minimum change for a specific tree topology. . Syst Zool 20, 406–416. [View Article]
    [Google Scholar]
  10. Gonzalez J. M., Saiz-Jimenez C. ( 2005 ). A simple fluorimetric method for the estimation of DNA-DNA relatedness between closely related microorganisms by thermal denaturation temperatures. . Extremophiles 9, 75–79. [View Article] [PubMed]
    [Google Scholar]
  11. Huss V. A. R., Festl H., Schleifer K. H. ( 1983 ). Studies on the spectrophotometric determination of DNA hybridization from renaturation rates. . Syst Appl Microbiol 4, 184–192. [View Article] [PubMed]
    [Google Scholar]
  12. Igarashi M., Takahashi Y., Shitara T., Nakamura H., Naganawa H., Miyake T., Akamatsu Y. ( 2005 ). Caprazamycins, novel lipo-nucleoside antibiotics, from Streptomyces sp. II. Structure elucidation of caprazamycins. . J Antibiot (Tokyo) 58, 327–337. [View Article] [PubMed]
    [Google Scholar]
  13. Jones K. L. ( 1949 ). Fresh isolates of actinomycetes in which the presence of sporogenous aerial mycelia is a fluctuating characteristic. . J Bacteriol 57, 141–145.[PubMed]
    [Google Scholar]
  14. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21–132. Edited by Munro H. N. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  15. Kelly K. L. ( 1964 ). Inter-Society Color Council – National Bureau of Standards Color Name Charts Illustrated with Centroid Colors. Washington, DC:: US Government Printing Office;.
     [Google Scholar]
  16. Kim B. Y., Zucchi T. D., Fiedler H. P., Goodfellow M. ( 2012a ). Streptomyces cocklensis sp. nov., a dioxamycin-producing actinomycete. . Int J Syst Evol Microbiol 62, 279–283. [View Article] [PubMed]
    [Google Scholar]
  17. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J. H. & other authors ( 2012b ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  18. Kroppenstedt R. M. ( 1982 ). Separation of bacterial menaquinones by HPLC using reverse phase (RP-18) and a silver-loaded ion exchanger. . J Liq Chromatogr 5, 2359–2367. [View Article]
    [Google Scholar]
  19. Kroppenstedt R. M., Goodfellow M. ( 2006 ). The family Thermomonosporaceae: Actinocorallia, Actinomadura, Spirillispora and Thermomonospora . . In The Prokaryotes, Archaea and Bacteria: Firmicutes, Actinomycetes, , 3rd edn., vol. 3, pp. 682–724. Edited by Dworkin M., Falkow S., Schleifer K. H., Stackebrandt E. . New York:: Springer;. [View Article]
    [Google Scholar]
  20. Labeda D. P. ( 1992 ). DNA-DNA hybridization in the systematics of Streptomyces . . Gene 115, 249–253. [View Article] [PubMed]
    [Google Scholar]
  21. Le Borgne S., Paniagua D., Vazquez-Duhalt R. ( 2008 ). Biodegradation of organic pollutants by halophilic bacteria and archaea. . J Mol Microbiol Biotechnol 15, 74–92. [View Article] [PubMed]
    [Google Scholar]
  22. Lechevalier M. P., Lechevalier H. ( 1970 ). Chemical composition as a criterion in the classification of aerobic actinomycetes. . Int J Syst Bacteriol 20, 435–443. [View Article]
    [Google Scholar]
  23. Li W.-J., Park D.-J., Tang S.-K., Wang D., Lee J.-C., Xu L.-H., Kim C.-J., Jiang C.-L. ( 2004 ). Nocardiopsis salina sp. nov., a novel halophilic actinomycete isolated from saline soil in China. . Int J Syst Evol Microbiol 54, 1805–1809. [View Article] [PubMed]
    [Google Scholar]
  24. Marhuenda-Egea F. C., Bonete M. J. ( 2002 ). Extreme halophilic enzymes in organic solvents. . Curr Opin Biotechnol 13, 385–389. [View Article] [PubMed]
    [Google Scholar]
  25. Meier-Kolthoff J. P., Göker M., Spröer C., Klenk H.-P. ( 2013 ). When should a DDH experiment be mandatory in microbial taxonomy?. Arch Microbiol 195, 413–418. [View Article] [PubMed]
    [Google Scholar]
  26. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal K., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233–241. [View Article]
    [Google Scholar]
  27. Nash P., Krent M. M. ( 1991 ). Culture media. . In Manual of Clinical Microbiology, , 5th edn., pp. 1268–1270. Edited by Ballows A., Hauser W. J., Herrmann K. L., Isenberg H. D., Shadomy H. J. . Washington, DC:: American Society for Microbiology;.
     [Google Scholar]
  28. Olano C., Gómez C., Pérez M., Palomino M., Pineda-Lucena A., Carbajo R. J., Braña A. F., Méndez C., Salas J. A. ( 2009 ). Deciphering biosynthesis of the RNA polymerase inhibitor streptolydigin and generation of glycosylated derivatives. . Chem Biol 16, 1031–1044. [View Article] [PubMed]
    [Google Scholar]
  29. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406–425.[PubMed]
    [Google Scholar]
  30. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
     [Google Scholar]
  31. Shiomi K., Hatae K., Hatano H., Matsumoto A., Takahashi Y., Jiang C. L., Tomoda H., Kobayashi S., Tanaka H., Omura S. ( 2005 ). A new antibiotic, antimycin Ag, produced by Streptomyces sp. K01-0031. . J Antibiot (Tokyo) 58, 74–78. [View Article] [PubMed]
    [Google Scholar]
  32. Shirling E. B., Gottlieb D. ( 1966 ). Methods for characterization of Streptomyces species. . Int J Syst Bacteriol 16, 313–340. [View Article]
    [Google Scholar]
  33. Staneck J. L., Roberts G. D. ( 1974 ). Simplified approach to identification of aerobic actinomycetes by thin-layer chromatography. . Appl Microbiol 28, 226–231.[PubMed]
    [Google Scholar]
  34. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  35. Tan G. Y. A., Ward A. C., Goodfellow M. ( 2006 ). Exploration of Amycolatopsis diversity in soil using genus-specific primers and novel selective media. . Syst Appl Microbiol 29, 557–569. [View Article] [PubMed]
    [Google Scholar]
  36. Tang S. K., Wang Y., Guan T. W., Lee J. C., Kim C. J., Li W. J. ( 2010 ). Amycolatopsis halophila sp. nov., a halophilic actinomycete isolated from a salt lake. . Int J Syst Evol Microbiol 60, 1073–1078. [View Article] [PubMed]
    [Google Scholar]
  37. Tang S. K., Zhi X. Y., Wang Y., Shi R., Lou K., Xu L. H., Li W. J. ( 2011 ). Haloactinopolyspora alba gen. nov., sp. nov., a halophilic filamentous actinomycete isolated from a salt lake, with proposal of Jiangellaceae fam. nov. and Jiangellineae subord. nov. . Int J Syst Evol Microbiol 61, 194–200. [View Article] [PubMed]
    [Google Scholar]
  38. Tatar D., Sazak A., Guven K., Cetin D., Sahin N. ( 2013 ). Amycolatopsis cihanbeyliensis sp. nov., a halotolerant actinomycete isolated from a salt mine. . Int J Syst Evol Microbiol 63, 3739–3743. [View Article] [PubMed]
    [Google Scholar]
  39. Wang Y., Tang S. K., Li Z., Lou K., Mao P. H., Jin X., Klenk H. P., Zhang L. X., Li W. J. ( 2011 ). Myceligenerans halotolerans sp. nov., an actinomycete isolated from a salt lake, and emended description of the genus Myceligenerans . . Int J Syst Evol Microbiol 61, 974–978. [View Article] [PubMed]
    [Google Scholar]
  40. Watve M. G., Tickoo R., Jog M. M., Bhole B. D. ( 2001 ). How many antibiotics are produced by the genus Streptomyces?. Arch Microbiol 176, 386–390. [View Article] [PubMed]
    [Google Scholar]
  41. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463–464. [View Article]
    [Google Scholar]
  42. Williams S. T., Goodfellow M., Alderson G., Wellington E. M. H., Sneath P. H. A., Sackin M. J. ( 1983 ). Numerical classification of Streptomyces and related genera. . J Gen Microbiol 129, 1743–1813.[PubMed]
    [Google Scholar]
  43. Yang C. X., Liu Y. P., Bao Q. H., Feng F. Y., Liu H. R., Zhang X. J., Zhao Y. L. ( 2012 ). Mongoliitalea lutea gen. nov., sp. nov., an alkaliphilic, halotolerant bacterium isolated from a haloalkaline lake. . Int J Syst Evol Microbiol 62, 647–653. [View Article] [PubMed]
    [Google Scholar]
  44. Yoon J.-H., Lee K.-C., Kho Y. H., Kang K. H., Kim C.-J., Park Y.-H. ( 2002 ). Halomonas alimentaria sp. nov., isolated from jeotgal, a traditional Korean fermented seafood. . Int J Syst Evol Microbiol 52, 123–130.[PubMed] [CrossRef]
    [Google Scholar]
  45. Zamanian S., Shahidi Bonjar G. H., Saadoun I. ( 2005 ). First report of antibacterial properties of a new strain of Streptomyces plicatus (strain 101) against Erwinia carotovora subsp. carotovora from Iran. . Biotechnology 4, 114–120. [View Article]
    [Google Scholar]
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Streptomyces iconiensis sp. nov. and Streptomyces smyrnaeus sp. nov., two halotolerant actinomycetes isolated from a salt lake and saltern
Int J Syst Evol Microbiol 64, 3126 (2014); https://doi.org/10.1099/ijs.0.062216-0
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