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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_8

sp. nov., isolated from a lagoon Free

Abstract

A brown-coloured bacterium, designated strain JA633, was purified from a photoheterotrophic enrichment culture obtained from black sand of a lagoon. Cells of strain JA633 were coccoid–spherical, Gram-stain-negative and motile by means of polar flagella. Strain JA633 had an obligate requirement for NaCl and could tolerate up to 4 % (w/v) NaCl. Internal photosynthetic membranes were present as vesicles. Photo-organoheterotrophy was the only growth mode observed. Strain JA633 contained bacteriochlorophyll and a major (>85 %) unidentified carotenoid of the spirilloxanthin series. Thiamine and -aminobenzoic acid were required for growth. Major fatty acids were Cω7/Cω6, C and Cω7/Cω6. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphotidylcholine and an unknown aminophospholipid were the major polar lipids in strain JA633. The DNA G+C content of strain JA633 was 64.5 mol%. Strain JA633 shared highest 16S rRNA gene sequence similarity with the type strains of (96.9 %), (96.3 %) and (96.2 %), which belonged to the class . However, phylogenetic analysis based on 16S rRNA gene sequences showed that strain JA633 formed a separate clade along with JA304 whereas the members of the genus remained as two distinct phylogenetic lineages. Based on morphological, physiological, chemotaxonomic and molecular evidence, strain JA633 was significantly different from the type strain of of the family . It is thus proposed that the strain be classified as a representative of a novel species, for which the name sp. nov. is proposed. The type strain is JA633 ( = KCTC 15337 = NBRC 109958).

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Funding
This study was supported by the:
  • UGC
  • DST
© 2014 IUMS
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2014-08-01
2025-01-21
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References

  1. Anil Kumar P., Srinivas T. N. R., Sasikala Ch., Ramana Ch. V., Imhoff J. F. ( 2008 ). Thiophaeococcus mangrovi gen. nov., sp. nov., a photosynthetic, marine gammaproteobacterium isolated from the Bhitarkanika mangrove forest of India. . Int J Syst Evol Microbiol 58, 2660–2664. [View Article] [PubMed]
    [Google Scholar]
  2. Hanada S., Takaichi S., Matsuura K., Nakamura K. ( 2002 ). Roseiflexus castenholzii gen. nov., sp. nov., a thermophilic, filamentous, photosynthetic bacterium that lacks chlorosomes. . Int J Syst Evol Microbiol 52, 187–193.[PubMed] [CrossRef]
    [Google Scholar]
  3. Hiraishi A., Hoshino Y. ( 1984 ). Distribution of rhodoquinone in Rhodospirillaceae and its taxonomic implications. . J Gen Appl Microbiol 30, 435–448. [View Article]
    [Google Scholar]
  4. Hiraishi A., Hoshino Y., Kitamura H. ( 1984 ). Isoprenoid quinone composition in the classification of Rhodospirillaceae . . J Gen Appl Microbiol 30, 197–210. [View Article]
    [Google Scholar]
  5. Imhoff J. F. ( 1984 ). Quinones of phototrophic purple bacteria. . FEMS Microbiol Lett 25, 85–89. [View Article]
    [Google Scholar]
  6. Imhoff J. F., Bias-Imhoff U. ( 1995 ). Lipids, quinones and fatty acids of anoxygenic phototrophic bacteria. . In Anoxygenic Photosynthetic Bacteria, vol. 2, pp. 179–205. Edited by Blackenship R. E., Madigan M. T., Bauer C. E. . Dordrecht:: Kluwer;. [View Article]
    [Google Scholar]
  7. Imhoff J. F., Caumette P. ( 2004 ). Recommended standards for the description of new species of anoxygenic phototrophic bacteria. . Int J Syst Evol Microbiol 54, 1415–1421. [View Article]
    [Google Scholar]
  8. Imhoff J. F., Kushner D. J., Kushwaha S. C., Kates M. ( 1982 ). Polar lipids in phototrophic bacteria of the Rhodospirillaceae and Chromatiaceae families. . J Bacteriol 150, 1192–1201.[PubMed]
    [Google Scholar]
  9. Imhoff J. F., Süling J., Petri R. ( 1998 ). Phylogenetic relationships among the Chromatiaceae, their taxonomic reclassification and description of the new genera Allochromatium, Halochromatium, Isochromatium, Marichromatium, Thiococcus, Thiohalocapsa and Thermochromatium . . Int J Syst Bacteriol 48, 1129–1143. [View Article] [PubMed]
    [Google Scholar]
  10. Kates M. ( 1972 ). Techniques in lipidology. . In Laboratory Techniques in Biochemistry and Molecular Biology, vol. 3, part 2, pp. 355–356. Edited by Work T. S., Work E. . New York:: American Elsevier Publishing Company;.
     [Google Scholar]
  11. Kates M. ( 1986 ). Techniques of lipidology: isolation, analysis and identification of lipids. . In Laboratory Techniques in Biochemistry and Molecular Biology, vol. 3, pp. 100–112. Edited by Burdon R. H., van Knippenberg P. H. . Amsterdam:: Elsevier;.
     [Google Scholar]
  12. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716–721. [View Article] [PubMed]
    [Google Scholar]
  13. Kimura M. ( 1980 ). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol 16, 111–120. [View Article] [PubMed]
    [Google Scholar]
  14. Lakshmi K. V. N. S., Sasikala Ch., Takaichi S., Ramana ChV. ( 2011 ). Phaeospirillum oryzae sp. nov., a spheroplast-forming, phototrophic alphaproteobacterium from a paddy soil. . Int J Syst Evol Microbiol 61, 1656–1661. [View Article] [PubMed]
    [Google Scholar]
  15. Logan N. A., Berge O., Bishop A. H., Busse H. J., De Vos P., Fritze D., Heyndrickx M., Kämpfer P., Rabinovitch L. & other authors ( 2009 ). Proposed minimal standards for describing new taxa of aerobic, endospore-forming bacteria. . Int J Syst Evol Microbiol 59, 2114–2121. [View Article] [PubMed]
    [Google Scholar]
  16. Marmur J. ( 1961 ). A procedure for the isolation of deoxyribonucleic acid from microorganisms. . J Mol Biol 3, 208–218. [View Article]
    [Google Scholar]
  17. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high performance liquid chromatography. . Int J Syst Bacteriol 39, 159–167. [View Article]
    [Google Scholar]
  18. Oren A., Duker S., Ritter S. ( 1996 ). The polar lipid composition of Walsby’s square bacterium. . FEMS Microbiol Lett 138, 135–140. [View Article]
    [Google Scholar]
  19. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
     [Google Scholar]
  20. Shivali K., Ramana V. V., Ramaprasad E. V., Sasikala Ch., Ramana Ch. V. ( 2011 ). Marichromatium litoris sp. nov. and Marichromatium chrysaorae sp. nov. isolated from beach sand and from a jelly fish (Chrysaora colorata). . Syst Appl Microbiol 34, 600–605. [View Article] [PubMed]
    [Google Scholar]
  21. Subhash Y., Sasikala Ch., Ramana Ch. V. ( 2013a ). Flavobacterium aquaticum sp. nov., isolated from a water sample of a rice field. . Int J Syst Evol Microbiol 63, 3463–3469. [View Article] [PubMed]
    [Google Scholar]
  22. Subhash Y., Tushar L., Sasikala Ch., Ramana Ch. V. ( 2013b ). Mongoliicoccus alkaliphilus sp. nov. and Litoribacter alkaliphilus sp. nov., isolated from salt pans. . Int J Syst Evol Microbiol 63, 3457–3462. [View Article] [PubMed]
    [Google Scholar]
  23. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 2731–2739. [View Article] [PubMed]
    [Google Scholar]
  24. Tindall B. J. ( 1990a ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199–202. [View Article]
    [Google Scholar]
  25. Tindall B. J. ( 1990b ). A comparative study of the lipid composition of Halobacterium saccharovorum from various sources. . Syst Appl Microbiol 13, 128–130. [View Article]
    [Google Scholar]
  26. Trüper H. G., Pfennig N. ( 1981 ). Characterization and identification of the anoxygenic phototrophic bacteria. . In The Prokaryotes: A Handbook on Habitats, Isolation, and Identification of Bacteria, vol. 1, pp. 298–312. Edited by Starr M. P., Stolp H., Trüper H. G., Balows A., Schlegel H. G. . New York:: Springer;. [View Article]
    [Google Scholar]
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Thiophaeococcus fuscus sp. nov., isolated from a lagoon
Int J Syst Evol Microbiol 64, 2528 (2014); https://doi.org/10.1099/ijs.0.062182-0
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