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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_9

sp. nov., isolated from the surface of rice straw Free

Abstract

An aerobic, Gram-stain-negative, rod-shaped bacterium, designated strain CC-LY845, was isolated from the surface of rice straw in Taiwan. Cells were non-motile, and no flagellum was detected. Comparison of 16S rRNA gene sequences indicated that the strain was phylogenetically related to species of the genus , with closest similarity to KCTC 23294 (97.6 %), KCTC 23652 (97.0 %) and LMG 24253 (96.7 %); other species showed lower levels of similarity (<96.6 %). The DNA–DNA relatedness of strain CC-LY845 and KCTC 23294 was 34.8±3.1 % (reciprocal value 39.2±2.2 %). Phylogenetic analysis based on the housekeeping and genes showed that the novel strain could be distinguished from KCTC 23294 (92.7 and 91.5 %, respectively) and other species of the genus . The temperature range for growth was 25–42 °C, the pH range was 5.0–9.0 and NaCl concentrations up to 4.0 % (w/v) were tolerated. Strain CC-LY845 did not form nodules on four different legumes, and the and genes were not detected by PCR. The major fatty acids were C and summed feature 8 (Cω7/Cω6). The polyamine pattern of strain CC-LY845 showed spermidine and putrescine as major polyamines. The predominant quinone system was ubiquinone 10 (Q-10). The DNA G+C content was 68.3±2.4 mol%. Base on its phylogenetic, phenotypic and chemotaxonomic features, strain CC-LY845 is proposed to represent a novel species within the genus , for which the name sp. nov. is proposed. The type strain is strain CC-LY845 ( = BCRC 80698 = JCM 19536).

  • Published Online:
Funding
This study was supported by the:
  • Ministry of Science and Technology
  • Council of Agriculture, Executive Yuan
  • Ministry of Education, Taiwan under the ATU plan
© 2014 IUMS
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2014-09-01
2025-03-24
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Rhizobium straminoryzae sp. nov., isolated from the surface of rice straw
Int J Syst Evol Microbiol 64, 2962 (2014); https://doi.org/10.1099/ijs.0.062117-0
/content/journal/ijsem/10.1099/ijs.0.062117-0
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