1887

Abstract

A novel bacterial strain, designated CJ29, was isolated from ginseng soil of Anseong in South Korea. Cells of strain CJ29 were Gram-stain-negative, facultatively anaerobic, rod-shaped and non-motile. Strain CJ29 grew optimally at 28–30 °C and pH 7.0. Based on 16S rRNA gene sequence analysis, strain CJ29 was shown to belong to the genus within the class and was related most closely to DCY21 (98.5 % similarity) and GH41-7 (98.2 %). DNA–DNA relatedness between strain CJ29 and its closest relatives was below 55.6 %. The predominant cellular fatty acids of strain CJ29 were iso-C, iso-C and iso-Cω9. The major polar lipids were diphosphatidylglycerol, phosphatidylethanolamine and phosphatidylglycerol. The major isoprenoid quinone was ubiquinone 8 (Q-8). The G+C content of the genomic DNA was 65.6 mol%. Phenotypic, genotypic and phylogenetic characteristics strongly supported the differentiation of strain CJ29 from related species of the genus . On the basis of data from this polyphasic taxonomic study, strain CJ29 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed. The type strain is CJ29 ( = KACC 17502 = JCM 19212).

Funding
This study was supported by the:
  • National Research Foundation of Korea (NRF)
  • Ministry of Education, Science and Technology (Award 2010-0013383)
  • National Institute of Biological Resources (NIBR)
Loading

Article metrics loading...

/content/journal/ijsem/10.1099/ijs.0.062034-0
2014-07-01
2024-04-20
Loading full text...

Full text loading...

/deliver/fulltext/ijsem/64/7/2193.html?itemId=/content/journal/ijsem/10.1099/ijs.0.062034-0&mimeType=html&fmt=ahah

References

  1. Ahmed K., Chohnan S., Ohashi H., Hirata T., Masaki T., Sakiyama F. ( 2003 ). Purification, bacteriolytic activity, and specificity of β-lytic protease from Lysobacter sp. IB-9374. . J Biosci Bioeng 95, 2734.[PubMed] [CrossRef]
    [Google Scholar]
  2. Baker G. C., Smith J. J., Cowan D. A. ( 2003 ). Review and re-analysis of domain-specific 16S primers. . J Microbiol Methods 55, 541555. [View Article] [PubMed]
    [Google Scholar]
  3. Christensen P., Cook F. D. ( 1978 ). Lysobacter, a new genus of nonfruiting, gliding bacteria with a high base ratio. . Int J Syst Bacteriol 28, 367393. [View Article]
    [Google Scholar]
  4. Collins M. D. ( 1985 ). Isoprenoid quinone analysis in classification and identification. . In Chemical Methods in Bacterial Systematics, pp. 267287. Edited by Minnikin M. G. D. E. . London:: Academic Press;.
    [Google Scholar]
  5. Ezaki T., Hashimoto Y., Yabuuchi E. ( 1989 ). Fluorometric deoxyribonucleic acid-deoxyribonucleic acid hybridization in microdilution wells as an alternative to membrane filter hybridization in which radioisotopes are used to determine genetic relatedness among bacterial strains. . Int J Syst Evol Microbiol 39, 224229.
    [Google Scholar]
  6. Folman L. B., De Klein M. J. E. M., Postma J., van Veen J. A. ( 2004 ). Production of antifungal compounds by Lysobacter enzymogenes isolate 3.1T8 under different conditions in relation to its efficacy as a biocontrol agent of Pythium aphanidermatum in cucumber. . Biol Control 31, 145154. [View Article]
    [Google Scholar]
  7. Fukuda W., Kimura T., Araki S., Miyoshi Y., Atomi H., Imanaka T. ( 2013 ). Lysobacter oligotrophicus sp. nov., isolated from an Antarctic freshwater lake in Antarctica. . Int J Syst Evol Microbiol 63, 33133318. [View Article] [PubMed]
    [Google Scholar]
  8. Jukes T. H., Cantor C. R. ( 1969 ). Evolution of protein molecules. . In Mammalian Protein Metabolism, vol. 3, pp. 21132. Edited by Munro H. N. . New York:: Academic Press;. [View Article]
    [Google Scholar]
  9. Kilic-Ekici O., Yuen G. Y. ( 2003 ). Induced resistance as a mechanism of biological control by Lysobacter enzymogenes strain C3. . Phytopathology 93, 11031110. [View Article] [PubMed]
    [Google Scholar]
  10. Kim O.-S., Cho Y.-J., Lee K., Yoon S.-H., Kim M., Na H., Park S.-C., Jeon Y. S., Lee J.-H. & other authors ( 2012 ). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62, 716721. [View Article] [PubMed]
    [Google Scholar]
  11. Kluge A. G., Farris F. S. ( 1969 ). Quantitative phyletics and the evolution of anurans. . Syst Zool 18, 132. [View Article]
    [Google Scholar]
  12. Liu M., Liu Y., Wang Y., Luo X., Dai J., Fang C. ( 2011 ). Lysobacter xinjiangensis sp. nov., a moderately thermotolerant and alkalitolerant bacterium isolated from a gamma-irradiated sand soil sample. . Int J Syst Evol Microbiol 61, 433437. [View Article] [PubMed]
    [Google Scholar]
  13. Luo G., Shi Z., Wang G. ( 2012 ). Lysobacter arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from iron-mined soil. . Int J Syst Evol Microbiol 62, 16591665. [View Article] [PubMed]
    [Google Scholar]
  14. Mesbah M., Premachandran U., Whitman W. B. ( 1989 ). Precise measurement of the G+C content of deoxyribonucleic acid by high-performance liquid chromatography. . Int J Syst Bacteriol 39, 159167. [View Article]
    [Google Scholar]
  15. Minnikin D. E., O’Donnell A. G., Goodfellow M., Alderson G., Athalye M., Schaal A., Parlett J. H. ( 1984 ). An integrated procedure for the extraction of bacterial isoprenoid quinones and polar lipids. . J Microbiol Methods 2, 233241. [View Article]
    [Google Scholar]
  16. Ryazanova L. P., Stepnaya O. A., Suzina N. E., Kulaev I. S. ( 2005 ). Antifungal action of the lytic enzyme complex from Lysobacter sp. XL1. . Process Biochem 40, 557564. [View Article]
    [Google Scholar]
  17. Saddler G. S., Bradbury J. F. ( 2005 ). Family I. Xanthomonadaceae fam. nov.. In Bergey’s Manual of Systematic Bacteriology, , 2nd edn., vol. 2, pp. 63122. Edited by Brenner D. J., Krieg N. R., Staley J. T., Garrity G. M. . New York:: Springer;. [View Article]
    [Google Scholar]
  18. Saitou N., Nei M. ( 1987 ). The neighbor-joining method: a new method for reconstructing phylogenetic trees. . Mol Biol Evol 4, 406425.[PubMed]
    [Google Scholar]
  19. Sasser M. ( 1990 ). Identification of bacteria by gas chromatography of cellular fatty acids, MIDI Technical Note 101. . Newark, DE:: MIDI Inc;.
    [Google Scholar]
  20. Spormann A. M. ( 1999 ). Gliding motility in bacteria: insights from studies of Myxococcus xanthus . . Microbiol Mol Biol Rev 63, 621641.[PubMed]
    [Google Scholar]
  21. Srinivasan S., Kim M. K., Sathiyaraj G., Kim H.-B., Kim Y.-J., Yang D.-C. ( 2010 ). Lysobacter soli sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 60, 15431547. [View Article] [PubMed]
    [Google Scholar]
  22. Tamura K., Peterson D., Peterson N., Stecher G., Nei M., Kumar S. ( 2011 ). mega5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods. . Mol Biol Evol 28, 27312739. [View Article] [PubMed]
    [Google Scholar]
  23. Ten L. N., Jung H.-M., Im W.-T., Yoo S.-A., Oh H.-M., Lee S.-T. ( 2009 ). Lysobacter panaciterrae sp. nov., isolated from soil of a ginseng field. . Int J Syst Evol Microbiol 59, 958963. [View Article] [PubMed]
    [Google Scholar]
  24. Thompson J. D., Gibson T. J., Plewniak F., Jeanmougin F., Higgins D. G. ( 1997 ). The clustal_x windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. . Nucleic Acids Res 25, 48764882. [View Article] [PubMed]
    [Google Scholar]
  25. Tindall B. J. ( 1990 ). Lipid composition of Halobacterium lacusprofundi . . FEMS Microbiol Lett 66, 199202. [View Article]
    [Google Scholar]
  26. Wayne L. G., Brenner D. J., Colwell R. R., Grimont P. A. D., Kandler O., Krichevsky M. I., Moore L. H., Moore W. E. C., Murray R. G. E. & other authors ( 1987 ). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol 37, 463464. [View Article]
    [Google Scholar]
  27. Wei D.-Q., Yu T.-T., Yao J.-C., Zhou E.-M., Song Z.-Q., Yin Y.-R., Ming H., Tang S.-K., Li W.-J. ( 2012 ). Lysobacter thermophilus sp. nov., isolated from a geothermal soil sample in Tengchong, south-west China. . Antonie van Leeuwenhoek 102, 643651. [View Article] [PubMed]
    [Google Scholar]
  28. Weon H.-Y., Kim B.-Y., Baek Y.-K., Yoo S.-H., Kwon S.-W., Stackebrandt E., Go S.-J. ( 2006 ). Two novel species, Lysobacter daejeonensis sp. nov. and Lysobacter yangpyeongensis sp. nov., isolated from Korean greenhouse soils. . Int J Syst Evol Microbiol 56, 947951. [View Article] [PubMed]
    [Google Scholar]
  29. Weon H.-Y., Kim B.-Y., Kim M.-K., Yoo S.-H., Kwon S.-W., Go S.-J., Stackebrandt E. ( 2007 ). Lysobacter niabensis sp. nov. and Lysobacter niastensis sp. nov., isolated from greenhouse soils in Korea. . Int J Syst Evol Microbiol 57, 548551. [View Article] [PubMed]
    [Google Scholar]
http://instance.metastore.ingenta.com/content/journal/ijsem/10.1099/ijs.0.062034-0
Loading
/content/journal/ijsem/10.1099/ijs.0.062034-0
Loading

Data & Media loading...

Supplements

Supplementary material 1

PDF
This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error