1887

Abstract

Strain JC216 was isolated from a contaminated Petri dish. Colonies were of pale yellow colour and cells were Gram-stain-negative, oxidase-positive and catalase-positive. Chitin, starch and gelatin were not hydrolysed. Strain JC216 contained Cω7/Cω6, Cω7/Cω6, C 2-OH and C as the major (≥8 %) fatty acids with minor amounts of C, C 2-OH, C 2-OH, C 2-OH, Cω6, Cω8 and Cω9. Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine and sphingoglycolipid were the major polar lipids. Minor amounts of unidentified amino lipids and unidentified lipids were also detected. The major hopanoids identified were bacterial hopane derivatives and diplopterol. Minor amounts of diploptene and an unidentified hopanoid were also present. Spermidine was the major polyamine with minor amounts of -homospermidine and putrescine. -Acetylglucosamine and fructose were identified as major cell-wall sugars along with minor amounts of mannose and galactose. The genomic DNA G+C content was 55 mol%. Comparisons of the16S rRNA gene sequence indicated that strain JC216 represents a member of the genus in the family within the class . Strain JC216 had a sequence similarity of 97.28 % with 03SU3-P and <96.71 % with other members of the family . Furthermore, strain JC216 had 33±1 % relatedness (based on DNA–DNA hybridization) with KCTC 23340 ( = 03SU3-P). Distinct morphological, physiological and genotypic differences from the previously described taxa support the classification of strain JC216 as a representative of a novel species in the genus , for which the name sp. nov. is proposed. The type strain is JC216 ( = KCTC 32445 = LMG 27671).

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2014-07-01
2019-10-15
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