A novel type of rock-weathering bacterium was isolated from weathered rock (tuff) surface collected from Dongxiang (Jiangxi, eastern China). Cells of strain G19T were Gram-reaction-positive, rod-shaped, endospore-forming and non-motile. The strain was aerobic, catalase- and oxidase-positive, and grew optimally at 30 °C and pH 7.0. On the basis of 16S rRNA gene sequence analysis, strain G19T was shown to belong to the genus Bacillus and the closest phylogenetic relatives were Bacillus aryabhattai B8W22T (97.4 %) and Bacillus megaterium IAM 13418T (97.1 %). The DNA G+C content was 36.7 mol% and the predominant respiratory quinone was MK-7. The major fatty acids were iso-C14 : 0, iso-C15 : 0 and anteiso-C15 : 0. The polar lipid profile of strain G19T contained phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol and an unidentified lipid. Based on the low level of DNA–DNA relatedness (ranging from 49.4 % to 55.0 %) to these type strains of species of the genus Bacillus and unique phenotypic characteristics, strain G19T represents a novel species of the genus Bacillus, for which the name Bacillusqingshengii sp. nov. is proposed. The type strain is G19T ( = CCTCC AB 2013273T = JCM 19454T).
Published Online:
Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 41071173)
CollinsM. D.,
JonesD.(1981). Distribution of isoprenoid quinone structural types in bacteria and their taxonomic implication. . Microbiol Rev45, 316–354.[PubMed]
CollinsM. D.,
PirouzT.,
GoodfellowM.,
MinnikinD. E.(1977). Distribution of menaquinones in actinomycetes and corynebacteria. . J Gen Microbiol100, 221–230. [View Article][PubMed]
De LeyJ.,
CattoirH.,
ReynaertsA.(1970). The quantitative measurement of DNA hybridization from renaturation rates. . Eur J Biochem12, 133–142. [View Article][PubMed]
DittmerJ. C.,
LesterR. L.(1964). A simple, specific spray for the detection of phospholipids on thin-layer chromatograms. . J Lipid Res5, 126–127.[PubMed]
HendricksC. W.,
DoyleJ. D.,
HugleyB.(1995). A new solid medium for enumerating cellulose-utilizing bacteria in soil. . Appl Environ Microbiol61, 2016–2019.[PubMed]
JacinH.,
MishkinA. R.(1965). Separation of carbohydrates on borate impregnated silica gel G plates. . J Chromatogr A18, 170–173. [View Article][PubMed]
KämpferP.(1994). Limits and possibilities of total fatty acid analysis for classification and identification of Bacillus species. . Syst Appl Microbiol17, 86–98. [View Article]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
KuykendallL. D.,
RoyM. A.,
O’NeillJ. J.,
DevineT. E.(1988). Fatty acids, antibiotic resistance, and deoxyribonucleic acid homology groups of Bradorhizobium japonicum
. . Int J Syst Bacteriol38, 358–361. [View Article]
LaneD. J.(1991). 16S/23S rRNA Sequencing. . In Nucleic Acid Techniques in Bacterial Systematics, pp. 115–175. Edited by
StackebrandtE.,
GoodfellowM.
. New York:: Wiley;.
MarmurJ.,
DotyP.(1962). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol5, 109–118. [View Article][PubMed]
NogiY.,
TakamiH.,
HorikoshiK.(2005). Characterization of alkaliphilic Bacillus strains used in industry: proposal of five novel species. . Int J Syst Evol Microbiol55, 2309–2315. [View Article][PubMed]
RossH. N. M.,
GrantW. D.,
HarrisJ. E.(1985). Lipids in archaebacterial taxonomy. . In Chemical Methods in Bacterial Systematics, pp. 289–299. Edited by
GoodfellowM.,
MinnikinD. E.
. London:: Academic Press;.
ShidaO.,
TakagiH.,
KadowakiK.,
NakamuraL. K.,
KomagataK.(1997). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus
. . Int J Syst Bacteriol47, 289–298. [View Article][PubMed]
ShivajiS.,
ChaturvediP.,
BegumZ.,
PindiP. K.,
ManoramaR.,
PadmanabanD. A.,
ShoucheY. S.,
PawarS.,
VaishampayanP.& other authors (2009).Janibacter hoylei sp. nov., Bacillus isronensis sp. nov. and Bacillus aryabhattai sp. nov., isolated from cryotubes used for collecting air from the upper atmosphere. . Int J Syst Evol Microbiol59, 2977–2986. [View Article][PubMed]
SureshK.,
PrabagaranS. R.,
SenguptaS.,
ShivajiS.(2004). Bacillus indicus sp. nov., an arsenic-resistant bacterium isolated from an aquifer in West Bengal, India. . Int J Syst Evol Microbiol54, 1369–1375. [View Article][PubMed]
TenL. N.,
ImW. T.,
KimM. K.,
KangM. S.,
LeeS. T.(2004). Development of a plate technique for screening of polysaccharide-degrading microorganisms by using a mixture of insoluble chromogenic substrates. . J Microbiol Methods56, 375–382. [View Article][PubMed]
TimkeM.,
Wang-LieuN. Q.,
AltendorfK.,
LipskiA.(2005). Community structure and diversity of biofilms from a beer bottling plant as revealed using 16S rRNA gene clone libraries. . Appl Environ Microbiol71, 6446–6452. [View Article][PubMed]
WayneL. G.,
BrennerD. J.,
ColwellR. R.,
GrimontP. A. D.,
KandlerO.,
KrichevskyM. I.,
MooreL. H.,
MooreW. E. C.,
MurrayR. G. E.& other authors (1987). International Committee on Systematic Bacteriology. Report of the ad hoc committee on reconciliation of approaches to bacterial systematics. . Int J Syst Bacteriol37, 463–464. [View Article]
WeonH. Y.,
KimB. Y.,
JoaJ. H.,
SonJ. A.,
SongM. H.,
KwonS. W.,
GoS. J.,
YoonS. H.(2008).Methylobacterium iners sp. nov. and Methylobacterium aerolatum sp. nov., isolated from air samples in Korea. . Int J Syst Evol Microbiol58, 93–96. [View Article][PubMed]