1887

Abstract

An aerobic, Gram-stain-negative, saffron-pigmented, non-motile bacterial strain KMM 9545, was isolated from a marine sand sediment sample obtained from the Sea of Japan seashore and characterized in a taxonomic study using a polyphasic approach. The 16S rRNA gene sequence of strain KMM 9545 showed a high level of similarity to species of the genus (95.1–96.2 %), the type strains of species of the genus (94.9–96.1 %) and members of the genus (94.8–96.1 %). Phylogenetic analysis based on the 16S rRNA gene sequences positioned strain KMM 9545 as a distinct lineage in the cluster comprising species of the genus . Strain KMM 9545 grew at temperatures between 5–36 °C and in the presence of 2–4 % (w/v) NaCl. It contained MK-6 as the predominant menaquinone and iso-C, iso-C, iso-C 3-OH, iso-C 3-OH and iso-C 2-OH as the major fatty acids. The genomic DNA G+C content was 31.3 mol%. On the basis of the phenotypic characteristics and phylogenetic distance, it can be concluded that strain KMM 9545 represents a novel species of the genus , for which the name sp. nov. is proposed. The type strain is KMM 9545 ( = NRIC 0921 = JCM 19808).

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2014-08-01
2020-01-26
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References

  1. Bernardet J.-F., Nakagawa Y., Holmes B..Subcommittee on the taxonomy of Flavobacterium and Cytophaga-like Flavobacterium and Cytophaga-like bacteria of the International Committee on Systematics of Prokaryotes ( 2002;). Proposed minimal standards for describing new taxa of the family Flavobacteriaceae and emended description of the family. . Int J Syst Evol Microbiol 52:, 1049–1070. [CrossRef][PubMed]
    [Google Scholar]
  2. Bowman J. P.. ( 2000;). Description of Cellulophaga algicola sp. nov., isolated from the surface of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov. Cellulophaga algicola sp. nov., isolated from the surfaces of Antarctic algae, and reclassification of Cytophaga uliginosa (ZoBell and Upham 1944) Reichenbach 1989 as Cellulophaga uliginosa comb. nov.. Int J Syst Evol Microbiol 50:, 1861–1868.[PubMed]
    [Google Scholar]
  3. Bruns A., Rohde M., Berthe-Corti L.. ( 2001;). Muricauda ruestringensis gen. nov., sp. nov., a facultatively anaerobic, appendaged bacterium from German North Sea intertidal sediment. . Int J Syst Evol Microbiol 51:, 1997–2006. [CrossRef][PubMed]
    [Google Scholar]
  4. Fautz E., Reichenbach H.. ( 1980;). A simple test for flexirubin-type pigments. . FEMS Microbiol Lett 8:, 87–91. [CrossRef]
    [Google Scholar]
  5. Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R.. (editors) ( 1994;). Methods for General and Molecular Bacteriology. Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  6. Jeong S. H., Park M. S., Jin H. M., Lee K., Park W., Jeon C. O.. ( 2013;). Aestuariibaculum suncheonense gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a tidal flat and emended descriptions of the genera Gaetbulibacter and Tamlana. . Int J Syst Evol Microbiol 63:, 332–338. [CrossRef][PubMed]
    [Google Scholar]
  7. Kim O. S., Cho Y. J., Lee K., Yoon S. H., Kim M., Na H., Park S. C., Jeon Y. S., Lee J. H.. & other authors ( 2012;). Introducing EzTaxon-e: a prokaryotic 16S rRNA gene sequence database with phylotypes that represent uncultured species. . Int J Syst Evol Microbiol 62:, 716–721. [CrossRef][PubMed]
    [Google Scholar]
  8. Lee S. D.. ( 2007;). Tamlana crocina gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae, isolated from beach sediment in Korea. . Int J Syst Evol Microbiol 57:, 764–769. [CrossRef][PubMed]
    [Google Scholar]
  9. Marmur J., Doty P.. ( 1962;). Determination of the base composition of deoxyribonucleic acid from its thermal denaturation temperature. . J Mol Biol 5:, 109–118. [CrossRef][PubMed]
    [Google Scholar]
  10. Owen R. J., Hill L. R., Lapage S. P.. ( 1969;). Determination of DNA base compositions from melting profiles in dilute buffers. . Biopolymers 7:, 503–516. [CrossRef][PubMed]
    [Google Scholar]
  11. Pearson W. R., Lipman D. J.. ( 1988;). Improved tools for biological sequence comparison. . Proc Natl Acad Sci U S A 85:, 2444–-2448. [CrossRef][PubMed]
    [Google Scholar]
  12. Pruesse E., Peplies J., Glöckner F. O.. ( 2012;). sina: accurate high-throughput multiple sequence alignment of ribosomal RNA genes. . Bioinformatics 28:, 1823–1829. [CrossRef][PubMed]
    [Google Scholar]
  13. Romanenko L. A., Uchino M., Frolova G. M., Mikhailov V. V.. ( 2007;). Marixanthomonas ophiurae gen. nov., sp. nov., a marine bacterium of the family Flavobacteriaceae isolated from a deep-sea brittle star. . Int J Syst Evol Microbiol 57:, 457–462. [CrossRef][PubMed]
    [Google Scholar]
  14. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V.. ( 2009;). Winogradskyella arenosi sp. nov., a member of the family Flavobacteriaceae isolated from marine sediments from the Sea of Japan. . Int J Syst Evol Microbiol 59:, 1443–1446. [CrossRef][PubMed]
    [Google Scholar]
  15. Romanenko L. A., Tanaka N., Frolova G. M., Mikhailov V. V.. ( 2010;). Arenicella xantha gen. nov., sp. nov., a gammaproteobacterium isolated from a marine sandy sediment. . Int J Syst Evol Microbiol 60:, 1832–1836. [CrossRef][PubMed]
    [Google Scholar]
  16. Sasser M.. ( 1990;). Identification of bacteria by gas chromatography of cellular fatty acids. , MIDI Technical Note 101. Newark, DE:: MIDI Inc;.
  17. Shida O., Takagi H., Kadowaki K., Nakamura L. K., Komagata K.. ( 1997;). Transfer of Bacillus alginolyticus, Bacillus chondroitinus, Bacillus curdlanolyticus, Bacillus glucanolyticus, Bacillus kobensis, and Bacillus thiaminolyticus to the genus Paenibacillus and emended description of the genus Paenibacillus. . Int J Syst Bacteriol 47:, 289–298. [CrossRef][PubMed]
    [Google Scholar]
  18. Smibert R. M., Krieg N. R.. ( 1994;). Phenotypic characterization. . In Methods for General and Molecular Bacteriology, pp. 607–655. Edited by Gerhardt P., Murray R. G. E., Wood W. A., Krieg N. R... Washington, DC:: American Society for Microbiology;.
    [Google Scholar]
  19. Stackebrandt E., Ebers J.. ( 2006;). Taxonomic parameters revisited: tarnished gold standards. . Microbiol Today 33:, 152–155.
    [Google Scholar]
  20. Stackebrandt E., Goebel B. M.. ( 1994;). Taxonomic note: a place for DNA-DNA reassociation and 16S rRNA sequence analysis in the present species definition in bacteriology. . Int J Syst Bacteriol 44:, 846–849. [CrossRef]
    [Google Scholar]
  21. Tamura K., Stecher G., Peterson D., Filipski A., Kumar S.. ( 2013;). mega6: molecular evolutionary genetics analysis version 6.0. . Mol Biol Evol 30:, 2725–2729. [CrossRef][PubMed]
    [Google Scholar]
  22. Yoon J. H., Kang S. J., Lee M. H., Oh T. K.. ( 2008;). Tamlana agarivorans sp. nov., isolated from seawater off Jeju Island in Korea. . Int J Syst Evol Microbiol 58:, 1892–1895. [CrossRef][PubMed]
    [Google Scholar]
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