Two actinomycete strains, designated TRM 49117T and TRM 49136T, were isolated from a hypersaline habitat in Xinjiang Province, north-west China and were characterized taxonomically by using a polyphasic study. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain TRM 49117T had 93.93 % similarity with the type strain Glycomyces halotolerans TRM 40137T (GenBank accession no. HQ651156) and TRM 49136T had 94.32 % similarity with G. halotolerans TRM 40137T. The 16S rRNA gene sequence similarity between the two new isolates was 93 %. The isolates contained meso-diaminopimelic acid as the diagnostic diamino acid and anteiso-C15 : 0, iso-C16 : 0 and anteiso-C17 : 0 as major cellular fatty acids. The predominant menaquinones of the isolates were MK-9(H4) and MK-9(H6). The whole-cell sugar patterns of these strains contained xylose and ribose, and strain TRM 49136T also contained arabinose. The polar lipid pattern of strain TRM 49117T comprised phosphatidylglycerol, diphosphatidylglycerol, phosphatidylinositol mannosides, phosphatidylcholine, phosphatidylinositol and three additional unknown phospholipids. The polar lipid pattern of strain TRM 49136T comprised phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, phosphatidylinositol, glycolipids and two phosphoglycolipids of unknown composition. Genotypic and phenotypic data confirmed that strains TRM 49117T and TRM 49136T represent two novel species, clearly different from related species of the genus Glycomyces, for which the names Glycomycesfuscus sp. nov. (type strain TRM 49117T = CCTCC AA 2013003T = NRRL B-59998T = KACC 17682T) and Glycomyces albus sp. nov. (type strain TRM 49136T = CCTCC AA 2013004T = NRRL B-24927T = KACC 17681T) are proposed.
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Funding
This study was supported by the:
National High Technology Research and Development Program of China
(Award 2012AA021705)
National Basic Research Program of China
(Award 2010CB134505)
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