A Gram-stain-negative, strictly aerobic, chemoheterotrophic marine bacterium, designated 20V17T, was isolated from a deep-sea hydrothermal vent chimney collected from the South-west Indian Ridge. Cells of strain 20V17T were motile, short rods, 1.2–1.8 µm in length and 0.5–0.7 µm in width. Growth was observed at between 20 and 37 °C (optimum 25 °C–28 °C), pH 5.0 and 8.0 (optimum pH 7.0) and 0.5 and 8 % (w/v) NaCl (optimum 1.5–2.0 % NaCl). The major fatty acids were C18 : 1ω7c (74.4 %), C19 : 0 cyclo ω8c (11 %), C18 : 0 (5.1 %) and C18 : 0 3-OH (2.8 %), and the polar lipid profile comprised diphosphatidylglycerol, phosphatidylethanolamine, an unidentified glycolipid and four unidentified phospholipids. Ubiquinone 10 was the major quinone. The G+C content of genomic DNA was 66.3 mol%. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain 20V17T belonged to the genus Defluviimonas and shared 96.5 and 96.1 % sequence similarity with Defluviimonas denitrificans D9-3T and Defluviimonas aestuarii BS14T, respectively. On the basis of the taxonomic data obtained in this study, strain 20V17T represents a novel species of the genus Defluviimonas, for which the name Defluviimonas indica sp. nov. is proposed. The type strain is 20V17T (CGMCC 1.10859T = JCM 17871T = MCCC 1A01802T).
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Funding
This study was supported by the:
National Natural Science Foundation of China
(Award 41106150)
National Program on Key Basic Research Project
(Award 2012CB417300 and 973 Program)
Natural Science Foundation of Fujian Province of China
(Award 2011J01209)
AchenbachL. A.,
CareyJ.,
MadiganM. T.(2001). Photosynthetic and phylogenetic primers for detection of anoxygenic phototrophs in natural environments. . Appl Environ Microbiol67, 2922–2926. [View Article][PubMed]
AltschulS. F.,
MaddenT. L.,
SchäfferA. A.,
ZhangJ.,
ZhangZ.,
MillerW.,
LipmanD. J.(1997). Gapped blast and psi-blast: a new generation of protein database search programs. . Nucleic Acids Res25, 3389–3402. [View Article][PubMed]
AusubelF. M.,
BrentR.,
KingstonR. E.,
MooreD. D.,
SeidmanJ. G.,
SmithJ. A.,
StruhlK.(editors) (1995).Short Protocols in Molecular Biology: a Compendium of Methods from Current Protocols in Molecular Biology, , 3rd edn.. New York:: Wiley;.
CytrynE.,
GelfandI.,
BarakY.,
van RijnJ.,
MinzD.(2003). Diversity of microbial communities correlated to physiochemical parameters in a digestion basin of a zero-discharge mariculture system. . Environ Microbiol5, 55–63. [View Article][PubMed]
FoeselB. U.,
DrakeH. L.,
SchrammA.(2011).Defluviimonas denitrificans gen. nov., sp. nov., and Pararhodobacter aggregans gen. nov., sp. nov., non-phototrophic Rhodobacteraceae from the biofilter of a marine aquaculture. . Syst Appl Microbiol34, 498–502. [View Article][PubMed]
KimuraM.(1980). A simple method for estimating evolutionary rates of base substitutions through comparative studies of nucleotide sequences. . J Mol Evol16, 111–120. [View Article][PubMed]
LiuC.,
ShaoZ.(2005).Alcanivorax dieselolei sp. nov., a novel alkane-degrading bacterium isolated from sea water and deep-sea sediment. . Int J Syst Evol Microbiol55, 1181–1186. [View Article][PubMed]
MathR. K.,
JinH. M.,
JeongS. H.,
JeonC. O.(2013).Defluviimonas aestuarii sp. nov., a marine bacterium isolated from a tidal flat, and emended description of the genus Defluviimonas Foesel et al. 2011. . Int J Syst Evol Microbiol63, 2895–2900. [View Article][PubMed]
WeryN.,
MoricetJ. M.,
CueffV.,
JeanJ.,
PignetP.,
LesongeurF.,
Cambon-BonavitaM. A.,
BarbierG.(2001).Caloranaerobacter azorensis gen. nov., sp. nov., an anaerobic thermophilic bacterium isolated from a deep-sea hydrothermal vent. . Int J Syst Evol Microbiol51, 1789–1796. [View Article][PubMed]