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INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY logo International Journal of Systematic and Evolutionary Microbiology

Volume 64, Issue Pt_6

sp. nov., an arsenite-resistant bacterium isolated from soil Free

Abstract

A Gram-reaction-negative, aerobic, motile, rod-shaped, arsenite [As(III)]-resistant bacterium, designated strain YW8, was isolated from agricultural soil. 16S rRNA gene sequence analysis showed over 97 % sequence similarity to strains of the environmental species , , , , and . However, the phylogenetic tree indicated that strain YW8 formed a separate clade from . DNA–DNA hybridization experiments showed that the DNA–DNA relatedness values between strain YW8 and its closest phylogenetic neighbours were below 24.2–35.5 %, which clearly separated the strain from these closely related species. The major cellular fatty acids of strain YW8 were C, C cyclo, Cω7, and summed feature 3(Cω6 and/or Cω7). The genomic DNA G+C content was 69.3 mol%, and the major respiratory quinone was ubiquinone-8. The predominant polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, three unknown phospholipids, an unknown polar lipid and phosphatidylserine. The major polyamines were 2-hydroxyputrescine and putrescine. On the basis of morphological, physiological and biochemical characteristics, phylogenetic position, DNA–DNA hybridization and chemotaxonomic data, strain YW8 is considered to represent a novel species of the genus , for which the name sp. nov. is proposed; the type strain is YW8 ( = CCTCC AB2012103 = KACC 16853).

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Funding
This study was supported by the:
  • National Natural Science Foundation of China (Award 31200087)
  • China Postdoctoral Science Foundation funded project (Award 2012M521094)
  • China Postdoctoral Science Special Foundation (Award 2013T60546)
  • Fundamental Research Funds for the Central Universities (Award Y0201200232)
  • Fundamental Research Funds for Environment and Plant Protection Institute, CATAS (Award 2010hzsZDZX001)
© 2014 IUMS
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2014-06-01
2025-01-25
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Xenophilus arseniciresistens sp. nov., an arsenite-resistant bacterium isolated from soil
Int J Syst Evol Microbiol 64, 1926 (2014); https://doi.org/10.1099/ijs.0.061515-0
/content/journal/ijsem/10.1099/ijs.0.061515-0
/content/journal/ijsem/10.1099/ijs.0.061515-0
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